PMID- 31115172
OWN - NLM
STAT- MEDLINE
DCOM- 20190903
LR  - 20190903
IS  - 1522-7278 (Electronic)
IS  - 1520-4081 (Linking)
VI  - 34
IP  - 8
DP  - 2019 Aug
TI  - LY303511 displays antiproliferation potential against oral cancer cells in vitro 
      and in vivo.
PG  - 958-967
LID - 10.1002/tox.22767 [doi]
AB  - LY303511 was developed as a negative control of LY294002 without 
      pan-phosphoinositide 3-kinase (PI3K) inhibition. We hypothesize LY303511 generate 
      reactive oxygen species (ROS) to induce apoptosis for killing oral cancer cells. 
      In MTS assay, LY303511 dose-responsively decreases survival in three kinds of 
      oral cancer cells but little damage to normal oral cells (HGF-1). Two oral cancer 
      cells (CAL 27 and SCC-9) with highly sensitivity to LY303511 were used. In 
      7-aminoactinomycin D (7AAD) assay, LY303511 slightly increases subG1 population 
      in oral cancer cells. In annexin V/7AAD and/or pancaspase assays, LY303511 
      induces apoptosis in oral cancer cells but HGF-1 cells remains in basal level. In 
      oxidative stress, LY303511 induces ROS and mitochondrial superoxide in oral 
      cancer cells. In 8-oxo-2'-deoxyguanosine assay, LY303511 induces oxidative DNA 
      damage in oral cancer cells. In zebrafish model, LY303511 inhibits CAL 
      27-xenografted tumor growth. Therefore, LY303511 displays antiproliferation 
      potential against oral cancer cells in vitro and in vivo.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Tang, Jen-Yang
AU  - Tang JY
AD  - Department of Radiation Oncology, Faculty of Medicine, College of Medicine, 
      Kaohsiung Medical University, Kaohsiung, Taiwan.
AD  - Department of Radiation Oncology, Kaohsiung Medical University Hospital, 
      Kaohsiung, Taiwan.
FAU - Xu, Yi-Hua
AU  - Xu YH
AD  - Department of Biomedical Science and Environmental Biology, Kaohsiung Medical 
      University, Kaohsiung, Taiwan.
FAU - Lin, Li-Ching
AU  - Lin LC
AD  - Department of Radiation Oncology, Chi-Mei Foundation Medical Center, Tainan, 
      Taiwan.
AD  - School of Medicine, Taipei Medical University, Taipei, Taiwan.
AD  - Chung Hwa University of Medical Technology, Tainan, Taiwan.
FAU - Ou-Yang, Fu
AU  - Ou-Yang F
AD  - Division of Breast Surgery and Department of Surgery, Kaohsiung Medical 
      University Hospital, Kaohsiung, Taiwan.
AD  - Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
FAU - Wu, Kuang-Han
AU  - Wu KH
AD  - Department of Biomedical Science and Environmental Biology, Kaohsiung Medical 
      University, Kaohsiung, Taiwan.
FAU - Tsao, Li-Yi
AU  - Tsao LY
AD  - Department of Biomedical Science and Environmental Biology, Kaohsiung Medical 
      University, Kaohsiung, Taiwan.
FAU - Yu, Tzu-Jung
AU  - Yu TJ
AD  - Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical 
      University, Kaohsiung, Taiwan.
FAU - Huang, Hurng-Wern
AU  - Huang HW
AD  - Institute of Biomedical Science, National Sun Yat-sen University, Kaohsiung, 
      Taiwan.
FAU - Wang, Hui-Ru
AU  - Wang HR
AD  - Institute of Biomedical Science, National Sun Yat-sen University, Kaohsiung, 
      Taiwan.
FAU - Liu, Wangta
AU  - Liu W
AD  - Department of Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan.
FAU - Chang, Hsueh-Wei
AU  - Chang HW
AUID- ORCID: 0000-0003-0068-2366
AD  - Department of Biomedical Science and Environmental Biology, Kaohsiung Medical 
      University, Kaohsiung, Taiwan.
AD  - Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
AD  - Institute of Medical Science and Technology, National Sun Yat-sen University, 
      Kaohsiung, Taiwan.
AD  - Regenerative Medicine and Cell Therapy Research Center, Kaohsiung Medical 
      University, Kaohsiung, Taiwan.
LA  - eng
GR  - MOHW108-TDU-B-212-124016/Health and Welfare Surcharge of Tobacco Products, the 
      Ministry of Health and Welfare, Taiwan, Republic of China/
GR  - KMUH105-5M24/Kaohsiung Medical University Hospital/
GR  - KMUH107-7R74/Kaohsiung Medical University Hospital/
GR  - MOST 107-2314-B-037-048/Ministry of Science and Technology, Taiwan/
GR  - MOST 107-2314-B-037-057/Ministry of Science and Technology, Taiwan/
GR  - MOST 107-2320-B-037-016/Ministry of Science and Technology, Taiwan/
GR  - MOST 107-2628-B-037-001/Ministry of Science and Technology, Taiwan/
GR  - MOST107-2311-B-037-001/Ministry of Science and Technology, Taiwan/
GR  - #NSYSUKMU 108-P001/National Sun Yat-sen University-KMU Joint Research Project/
GR  - NSYSUKMU 108-P001/National Sun Yat-sen University-KMU Joint Research Project/
GR  - Ministry of Health and Welfare, Taiwan, Republic of China/
GR  - Health and Welfare Surcharge of Tobacco Products/
GR  - 108-CCH-KMU-008/ChangHua Christian Hospital-KMU Fund/
GR  - Kaohsiung Medical University/
GR  - Ministry of Science and Technology/
PT  - Journal Article
DEP - 20190521
PL  - United States
TA  - Environ Toxicol
JT  - Environmental toxicology
JID - 100885357
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Chromones)
RN  - 0 (Piperazines)
RN  - 0 (Reactive Oxygen Species)
RN  - 154447-38-8 (LY 303511)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/*therapeutic use
MH  - Apoptosis
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Chromones/*therapeutic use
MH  - DNA Damage
MH  - Humans
MH  - Mouth Neoplasms/*drug therapy/metabolism/pathology
MH  - Oxidative Stress
MH  - Piperazines/*therapeutic use
MH  - Reactive Oxygen Species/metabolism
MH  - Zebrafish
OTO - NOTNLM
OT  - LY303511
OT  - oral cancer
OT  - oxidative stress
OT  - preferential killing
OT  - zebrafish xenograft model
EDAT- 2019/05/23 06:00
MHDA- 2019/09/04 06:00
CRDT- 2019/05/23 06:00
PHST- 2019/02/14 00:00 [received]
PHST- 2019/04/17 00:00 [revised]
PHST- 2019/04/24 00:00 [accepted]
PHST- 2019/05/23 06:00 [pubmed]
PHST- 2019/09/04 06:00 [medline]
PHST- 2019/05/23 06:00 [entrez]
AID - 10.1002/tox.22767 [doi]
PST - ppublish
SO  - Environ Toxicol. 2019 Aug;34(8):958-967. doi: 10.1002/tox.22767. Epub 2019 May 
      21.