PMID- 31115509
OWN - NLM
STAT- MEDLINE
DCOM- 20191230
LR  - 20200225
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 20
IP  - 1
DP  - 2019 Jul
TI  - Neuregulin‑1 impacting bone marrow mesenchymal stem cell migration is conducive 
      to functional recovery following spinal cord injury.
PG  - 41-48
LID - 10.3892/mmr.2019.10217 [doi]
AB  - The present study was designed to investigate the effect of neuregulin‑1 (NRG1) 
      on the migration of rat bone marrow mesenchymal stem cells (BMSCs) and evaluate 
      the role of NRG1 in the functional recovery following spinal cord injury (SCI). 
      Firstly, the effect of NRG1 on the mRNA expression of Snail in the BMSCs was 
      detected by reverse transcription‑quantitative polymerase chain reaction 
      (RT‑qPCR) analysis; secondly, the BMSCs were transfected with a 
      Snail‑overexpression plasmid (pBabe‑puro‑Snail) and the expression levels of 
      Snail and matrix metalloptoreinase‑2 (MMP‑2) were detected by RT‑qPCR and western 
      blot analyses; thirdly, the cell proliferation and migration of BMSCs modified 
      with pBabe‑puro‑Snail were detected by methyl thiazolyl tetrazolium and migration 
      assays, respectively; finally, functional recovery of SCI was assessed using the 
      Basso, Beattie, and Bresnahan rating scales. The results showed that NRG1 
      concentration‑dependently promoted the expression of Snail with a peak at 
      40 ng/ml and 48 h; NRG1 enhanced the promoting effect of Snail on the expression 
      of MMP‑2; the overexpression of Snail did not enhance the cell growth of the 
      BMSCs. The NRG1‑modified BMSCs promoted the functional recovery of SCI. These 
      results suggested that NRG1 significantly promoted the expression of MMP‑2 by 
      upregulating the expression of Snail, and enhanced cell migration of the BMSCs 
      conducive to the functional recovery of SCI.
FAU - Yi, Xi-Meng
AU  - Yi XM
AD  - Department of Orthopedics, The First Affiliated Hospital of China Medical 
      University, Shenyang, Liaoning 110001, P.R. China.
FAU - Chen, Yi
AU  - Chen Y
AD  - Department of Orthopedics, The First Affiliated Hospital of China Medical 
      University, Shenyang, Liaoning 110001, P.R. China.
FAU - Tu, Guan-Jun
AU  - Tu GJ
AD  - Department of Orthopedics, The First Affiliated Hospital of China Medical 
      University, Shenyang, Liaoning 110001, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190509
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Neuregulin-1)
RN  - 0 (SNAI1 protein, human)
RN  - 0 (Snail Family Transcription Factors)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.24 (Mmp2 protein, rat)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/genetics
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Disease Models, Animal
MH  - Gene Expression Regulation/genetics
MH  - Humans
MH  - Matrix Metalloproteinase 2/genetics
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Neuregulin-1/*genetics
MH  - Plasmids/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recovery of Function/genetics
MH  - Snail Family Transcription Factors/genetics
MH  - Spinal Cord/*growth & development/metabolism/pathology
MH  - Spinal Cord Injuries/*genetics/pathology/therapy
MH  - Transfection
PMC - PMC6580016
EDAT- 2019/05/23 06:00
MHDA- 2019/12/31 06:00
PMCR- 2019/05/09
CRDT- 2019/05/23 06:00
PHST- 2018/02/23 00:00 [received]
PHST- 2018/10/15 00:00 [accepted]
PHST- 2019/05/23 06:00 [pubmed]
PHST- 2019/12/31 06:00 [medline]
PHST- 2019/05/23 06:00 [entrez]
PHST- 2019/05/09 00:00 [pmc-release]
AID - mmr-20-01-0041 [pii]
AID - 10.3892/mmr.2019.10217 [doi]
PST - ppublish
SO  - Mol Med Rep. 2019 Jul;20(1):41-48. doi: 10.3892/mmr.2019.10217. Epub 2019 May 9.