PMID- 31115927
OWN - NLM
STAT- MEDLINE
DCOM- 20200218
LR  - 20200218
IS  - 1554-527X (Electronic)
IS  - 0736-0266 (Linking)
VI  - 37
IP  - 10
DP  - 2019 Oct
TI  - Restoration of Cellular Proliferation and Characteristics of Human Tenocytes by 
      Vitamin D.
PG  - 2241-2248
LID - 10.1002/jor.24352 [doi]
AB  - Vitamin D (Vit D) increases calcium absorption in the intestine after binding to 
      the Vit D receptor (VDR). The VDR has also been identified in muscle cells. Vit D 
      supplementation resulted in improved muscle strength. However, there is a paucity 
      of studies of the role of Vit D on tenocytes. We investigated the effects of Vit 
      D on damaged tenocytes. Human tenocytes were treated with dexamethasone (Dex) to 
      induce cell injury. Expression of the tenocyte-related markers tenomodulin 
      (Tnmd), tenascin C (Tnc), scleraxis (Scx), mohawk (Mkx), and collagen (Col) 1 and 
      3 were measured. Then, tenocytes were cotreated with Vit D. 1-alpha-Hydroxylase and 
      VDR were explored in tenocytes. With 10 muM Dex, the growth of tenocytes was 
      significantly inhibited, and the gene expression of Tnmd, Tnc, Scx, Mkx, Col 1 
      and 3 also decreased. When tenocytes were cotreated with Vit D, cell 
      proliferation recovered in a dose-dependent manner, and the expression of TNMD 
      and Col 1 improved. When studying the mechanisms of the effects of Vit D on 
      tenocytes, reactive oxygen species produced by Dex decreased with Vit D, and the 
      phosphorylation of extracellular signal-regulated kinase and p38 was stimulated 
      by Vit D cotreatment. 1-alpha-Hydroxylase and VDR were found in tenocytes, indicating 
      that the cells have the ability to use an inactive form of Vit D and interact 
      with it. Vit D is known to perform diverse actions and its protective effects on 
      tenocytes suggest its beneficial role in tendon in addition to muscle and bone. (c) 
      2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop 
      Res 37:2241-2248, 2019.
CI  - (c) 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
FAU - Min, Kyunghoon
AU  - Min K
AUID- ORCID: 0000-0003-3357-9795
AD  - Department of Rehabilitation Medicine, CHA Bundang Medical Center, CHA University 
      School of Medicine, Seongnam, Republic of Korea.
FAU - Lee, Ji Min
AU  - Lee JM
AD  - Department of Biotechnology, CHA University, Seongnam, 13488, Republic of Korea.
FAU - Kim, Mi Jin
AU  - Kim MJ
AD  - Department of Biotechnology, CHA University, Seongnam, 13488, Republic of Korea.
FAU - Jung, Sang Youn
AU  - Jung SY
AD  - Department of Internal Medicine, Division of Rheumatology, CHA Bundang Medical 
      Center, CHA University School of Medicine, Seongnam, Republic of Korea.
FAU - Kim, Kyung-Soo
AU  - Kim KS
AD  - Department of Internal Medicine, Division of Endocrinology and Metabolism, CHA 
      Bundang Medical Center, CHA University School of Medicine, Seongnam, Republic of 
      Korea.
FAU - Lee, Soonchul
AU  - Lee S
AD  - Department of Orthopaedic Surgery, CHA Bundang Medical Center, CHA University 
      School of Medicine, Seongnam, Republic of Korea.
FAU - Choi, Yong-Soo
AU  - Choi YS
AUID- ORCID: 0000-0001-8445-8067
AD  - Department of Biotechnology, CHA University, Seongnam, 13488, Republic of Korea.
LA  - eng
GR  - HR16C1559/Korea Health Industry Development Institute/International
GR  - 2017R1C1B1006702/National Research Foundation of Korea/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190625
PL  - United States
TA  - J Orthop Res
JT  - Journal of orthopaedic research : official publication of the Orthopaedic 
      Research Society
JID - 8404726
RN  - 0 (Bone Density Conservation Agents)
RN  - 0 (Glucocorticoids)
RN  - 0 (Receptors, Calcitriol)
RN  - 1406-16-2 (Vitamin D)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 1.14.- (Steroid Hydroxylases)
RN  - EC 1.14.- (vitamin D 1-alpha hydroxylase)
SB  - IM
MH  - Bone Density Conservation Agents/*therapeutic use
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Dexamethasone/*adverse effects
MH  - Drug Evaluation, Preclinical
MH  - Glucocorticoids/*adverse effects
MH  - Humans
MH  - MAP Kinase Signaling System/drug effects
MH  - Receptors, Calcitriol/metabolism
MH  - Steroid Hydroxylases/metabolism
MH  - Tenocytes/*drug effects/metabolism
MH  - Vitamin D/*therapeutic use
OTO - NOTNLM
OT  - 1-alpha-hydroxylase
OT  - dexamethasone
OT  - tenocyte
OT  - vitamin D
OT  - vitamin D receptor
EDAT- 2019/05/23 06:00
MHDA- 2020/02/19 06:00
CRDT- 2019/05/23 06:00
PHST- 2018/11/20 00:00 [received]
PHST- 2019/05/14 00:00 [accepted]
PHST- 2019/05/23 06:00 [pubmed]
PHST- 2020/02/19 06:00 [medline]
PHST- 2019/05/23 06:00 [entrez]
AID - 10.1002/jor.24352 [doi]
PST - ppublish
SO  - J Orthop Res. 2019 Oct;37(10):2241-2248. doi: 10.1002/jor.24352. Epub 2019 Jun 
      25.