PMID- 31116499
OWN - NLM
STAT- MEDLINE
DCOM- 20200903
LR  - 20231013
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Print)
IS  - 1549-3296 (Linking)
VI  - 107
IP  - 10
DP  - 2019 Oct
TI  - Time-of-flight secondary ion mass spectrometry three-dimensional imaging of 
      surface modifications in poly(caprolactone) scaffold pores.
PG  - 2195-2204
LID - 10.1002/jbm.a.36729 [doi]
AB  - Scaffolds composed of synthetic polymers such as poly(caprolactone) (PCL) are 
      widely used for the support and repair of tissues in biomedicine. Pores are 
      common features in scaffolds as they facilitate cell penetration. Various surface 
      modifications can be performed to promote key biological responses to these 
      scaffolds. However, verifying the chemistry of these materials post surface 
      modification is problematic due to the combination of three-dimensional (3D) 
      topography and surface sensitivity. Time-of-flight secondary ion mass 
      spectrometry (ToF-SIMS) is commonly used to correlate surface chemistry with cell 
      response. In this study, 3D imaging mass spectrometry analysis of surface 
      modified synthetic polymer scaffolds is demonstrated using PCL porous scaffold, a 
      pore filling polymer sample preparation, and 3D imaging ToF-SIMS. We apply a 
      simple sample preparation procedure, filling the scaffold pores with a poly(vinyl 
      alcohol)/glycerol mixture to remove topographic influence on image quality. This 
      filling method allows the scaffold (PCL) and filler secondary ions to be 
      reconstructed into a 3D chemical image of the pore. Furthermore, we show that 
      surface modifications in the pores of synthetic polymer scaffolds can be mapped 
      in 3D. Imaging of "dry" and "wet" surface modifications is demonstrated as well 
      as a comparison of surface modifications with relatively strong ToF-SIMS peaks 
      (fluorocarbon films [FC]) and to more biologically relevant surface modification 
      of a protein (bovine serum albumin [BSA]). We demonstrate that surface 
      modifications can be imaged in 3D showing that characteristic secondary ions 
      associated with FC and BSA are associated with C(3) F(8) plasma treatment and 
      BSA, respectively within the pore.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Taylor, Michael J
AU  - Taylor MJ
AUID- ORCID: 0000-0001-6926-3720
AD  - NESAC/BIO, Department of Bioengineering, University of Washington, Seattle, 
      Washington.
FAU - Graham, Daniel J
AU  - Graham DJ
AD  - NESAC/BIO, Department of Bioengineering, University of Washington, Seattle, 
      Washington.
FAU - Gamble, Lara J
AU  - Gamble LJ
AD  - NESAC/BIO, Department of Bioengineering, University of Washington, Seattle, 
      Washington.
LA  - eng
GR  - P41 EB002027/EB/NIBIB NIH HHS/United States
GR  - NESACBIO NIH P41 EB002027/EB/NIBIB NIH HHS/United States
GR  - NNCI-1542101/National Science Foundation/International
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20190602
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Fluorocarbons)
RN  - 0 (Plasma Gases)
RN  - 0 (Polyesters)
RN  - 24980-41-4 (polycaprolactone)
RN  - 27432CM55Q (Serum Albumin, Bovine)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Fluorocarbons/chemistry
MH  - *Imaging, Three-Dimensional
MH  - Photoelectron Spectroscopy
MH  - Plasma Gases/chemistry
MH  - Polyesters/*chemistry
MH  - Porosity
MH  - Serum Albumin, Bovine/chemistry
MH  - *Spectrometry, Mass, Secondary Ion
MH  - Surface Properties
MH  - Tissue Scaffolds/*chemistry
PMC - PMC6690353
MID - NIHMS1032441
OTO - NOTNLM
OT  - 3D
OT  - ToF-SIMS
OT  - XPS
OT  - imaging
OT  - scaffold
OT  - surface modifications
EDAT- 2019/05/23 06:00
MHDA- 2020/09/04 06:00
PMCR- 2020/10/01
CRDT- 2019/05/23 06:00
PHST- 2019/02/08 00:00 [received]
PHST- 2019/05/14 00:00 [revised]
PHST- 2019/05/20 00:00 [accepted]
PHST- 2019/05/23 06:00 [pubmed]
PHST- 2020/09/04 06:00 [medline]
PHST- 2019/05/23 06:00 [entrez]
PHST- 2020/10/01 00:00 [pmc-release]
AID - 10.1002/jbm.a.36729 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2019 Oct;107(10):2195-2204. doi: 10.1002/jbm.a.36729. Epub 
      2019 Jun 2.