PMID- 31119513
OWN - NLM
STAT- MEDLINE
DCOM- 20200723
LR  - 20200928
IS  - 2629-3277 (Electronic)
IS  - 2629-3277 (Linking)
VI  - 15
IP  - 4
DP  - 2019 Aug
TI  - Umbilical Cord Cell Therapy Improves Spatial Memory in Aging Rats.
PG  - 612-617
LID - 10.1007/s12015-019-09895-2 [doi]
AB  - There is a growing interest in the potential of adult stem cells for implementing 
      regenerative medicine in the brain. We assessed the effect of 
      intracerebroventricular (icv) administration of human umbilical cord perivascular 
      cells (HUCPVCs) on spatial memory of senile (27 mo) female rats, using intact 
      senile counterparts as controls. Approximately one third of the animals were 
      injected in the lateral ventricles with a suspension containing 4.8 X 10(5) 
      HUCPVC in 8 mul per side. The other third received 4.8 X 10(5) transgenic HUCPVC 
      overexpressing Insulin-like growth factor-1 (IGF-1) and the last third of the 
      rats received no treatment. Spatial memory performance was evaluated using a 
      modified version of the Barnes maze test. In order to evaluate learning ability 
      as well as spatial memory retention, we assessed the time spent (permanence) by 
      animals in goal sector 1 (GS(1)) and 3 (GS(3)) when the escape box was removed. 
      Fluorescence microscopy revealed the prescence of Dil-labeled HUCPVC in coronal 
      sections of treated brains. The HUCPVC were located in close contact with the 
      ependymal cells with only a few labeled cells migrating into the brain 
      parenchyma. After treatment with naive or IGF-1 transgenic HUCPVC, permanence in 
      GS(1) and GS(3) increased significantly whereas there were no changes in the 
      intact animals. We conclude that HUCPVC injected icv are effective to improve 
      some components of spatial memory in senile rats. The ready accessibility of 
      HUCPVC constitutes a significant incentive to continue the exploration of their 
      therapeutic potential on neurodegenerative diseases.
FAU - Lehmann, Marianne
AU  - Lehmann M
AD  - INIBIOLP-Pathology B, School of Medicine, National University of La Plata, Calles 
      60 y 120, 1900, La Plata, Argentina.
AD  - Department of Histology and of Embryology B, School of Medicine, National 
      University of La Plata, La Plata, Buenos Aires, Argentina.
FAU - Zappa-Villar, Maria F
AU  - Zappa-Villar MF
AD  - INIBIOLP-Pathology B, School of Medicine, National University of La Plata, Calles 
      60 y 120, 1900, La Plata, Argentina.
AD  - Department of Histology and of Embryology B, School of Medicine, National 
      University of La Plata, La Plata, Buenos Aires, Argentina.
FAU - Garcia, Mariana G
AU  - Garcia MG
AD  - Gene Therapy Laboratory, IIMT, Facultad de Ciencias Biomedicas, CONICET, 
      Universidad Austral, Buenos Aires, Argentina.
FAU - Mazzolini, Guillermo
AU  - Mazzolini G
AD  - Gene Therapy Laboratory, IIMT, Facultad de Ciencias Biomedicas, CONICET, 
      Universidad Austral, Buenos Aires, Argentina.
FAU - Canatelli-Mallat, Martina
AU  - Canatelli-Mallat M
AD  - INIBIOLP-Pathology B, School of Medicine, National University of La Plata, Calles 
      60 y 120, 1900, La Plata, Argentina.
AD  - Department of Histology and of Embryology B, School of Medicine, National 
      University of La Plata, La Plata, Buenos Aires, Argentina.
FAU - Morel, Gustavo R
AU  - Morel GR
AD  - INIBIOLP-Pathology B, School of Medicine, National University of La Plata, Calles 
      60 y 120, 1900, La Plata, Argentina.
AD  - Department of Histology and of Embryology B, School of Medicine, National 
      University of La Plata, La Plata, Buenos Aires, Argentina.
FAU - Reggiani, Paula C
AU  - Reggiani PC
AD  - INIBIOLP-Pathology B, School of Medicine, National University of La Plata, Calles 
      60 y 120, 1900, La Plata, Argentina.
AD  - Department of Histology and of Embryology B, School of Medicine, National 
      University of La Plata, La Plata, Buenos Aires, Argentina.
FAU - Goya, Rodolfo G
AU  - Goya RG
AUID- ORCID: 0000-0002-5469-5499
AD  - INIBIOLP-Pathology B, School of Medicine, National University of La Plata, Calles 
      60 y 120, 1900, La Plata, Argentina. goya@isis.unlp.edu.ar.
AD  - Department of Histology and of Embryology B, School of Medicine, National 
      University of La Plata, La Plata, Buenos Aires, Argentina. goya@isis.unlp.edu.ar.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Stem Cell Rev Rep
JT  - Stem cell reviews and reports
JID - 101752767
SB  - IM
MH  - *Aging
MH  - Animals
MH  - Brain/*physiopathology
MH  - *Cell Transplantation
MH  - Female
MH  - Humans
MH  - Memory Disorders/pathology/physiopathology/*therapy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Spatial Memory
MH  - *Umbilical Cord
OTO - NOTNLM
OT  - Brain aging
OT  - Hippocampus
OT  - Spatial memory
OT  - Stem cells
OT  - Umbilical cord
EDAT- 2019/05/24 06:00
MHDA- 2020/07/24 06:00
CRDT- 2019/05/24 06:00
PHST- 2019/05/24 06:00 [pubmed]
PHST- 2020/07/24 06:00 [medline]
PHST- 2019/05/24 06:00 [entrez]
AID - 10.1007/s12015-019-09895-2 [pii]
AID - 10.1007/s12015-019-09895-2 [doi]
PST - ppublish
SO  - Stem Cell Rev Rep. 2019 Aug;15(4):612-617. doi: 10.1007/s12015-019-09895-2.