PMID- 3112116
OWN - NLM
STAT- MEDLINE
DCOM- 19870828
LR  - 20131121
IS  - 0004-5756 (Print)
IS  - 0004-5756 (Linking)
VI  - 70
IP  - 3
DP  - 1987 May-Jun
TI  - Determination of ergotamine tartrate in tablets by liquid chromatography with 
      fluorescence detection.
PG  - 538-40
AB  - A method is presented for the liquid chromatographic (LC) determination of 
      ergotamine tartrate in tablets that is applicable even in the presence of other 
      ingredients such as phenobarbital, belladonna alkaloids, and caffeine. The sample 
      is transferred to a volumetric flask, a small volume of formic acid is added to 
      dissolve and stabilize the ergotamine, and the solution is diluted to volume with 
      methanol. The solution is mixed and filtered through paper. The LC system 
      consists of a Rheodyne injector fitted with a 20 microL loop and a C18 reverse 
      phase column; the mobile phase is acetonitrile-water-triethylamine (700 + 300 + 
      0.5). Ergotamine tartrate is determined fluorometrically at an excitation 
      wavelength of 250 nm and an emission wavelength of 430 nm. Recovery studies were 
      conducted at the 0.3 and 1.0 mg levels. Average recoveries were 99.6 and 100.8%, 
      respectively; relative standard deviations (RSDs) were 1.08 and 2.21%, 
      respectively. Some commercial preparations containing ergotamine tartrate in 
      combination with other ingredients were also analyzed. The RSDs for 5 
      determinations of each of 2 ground composites were 0.09 and 0.34%.
FAU - Cieri, U R
AU  - Cieri UR
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Assoc Off Anal Chem
JT  - Journal - Association of Official Analytical Chemists
JID - 7505559
RN  - 0 (Ergotamines)
RN  - 0 (Tablets)
RN  - PR834Q503T (Ergotamine)
SB  - IM
MH  - Chromatography, Liquid
MH  - Ergotamine
MH  - Ergotamines/*analysis
MH  - Spectrometry, Fluorescence
MH  - Tablets
EDAT- 1987/05/01 00:00
MHDA- 1987/05/01 00:01
CRDT- 1987/05/01 00:00
PHST- 1987/05/01 00:00 [pubmed]
PHST- 1987/05/01 00:01 [medline]
PHST- 1987/05/01 00:00 [entrez]
PST - ppublish
SO  - J Assoc Off Anal Chem. 1987 May-Jun;70(3):538-40.