PMID- 31121496
OWN - NLM
STAT- MEDLINE
DCOM- 20200818
LR  - 20200818
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Linking)
VI  - 120
DP  - 2019 Aug
TI  - Cytokine expression profiles in cervical mucus from patients with cervical cancer 
      and its precursor lesions.
PG  - 210-219
LID - S1043-4666(19)30129-2 [pii]
LID - 10.1016/j.cyto.2019.05.011 [doi]
AB  - Human papillomavirus (HPV) infection can persist in the cervical epithelium 
      without provoking a strong host immune response, leading to the development of 
      cervical cancer. Cytokines, which mediate innate and adaptive immune activities, 
      are secreted in the cervical mucus; however, there is currently no appropriate 
      method for assessing cytokine levels in mucus specimens. Here, we employed 
      multiplexed bead-based immunoassays to examine cytokine levels in cervical mucus 
      using both weighted-volume and total protein concentration methods to adjust for 
      different specimen volumes in individual patients. Out of 18 cytokines initially 
      examined in the primary cohort patient group (n = 28), 14 were detected in more 
      than 10% of the samples. Of these 14 cytokines, expression levels of interferon 
      (IFN)-gamma, granulocyte-macrophage colony-stimulating factor (GM-CSF), RANTES, and 
      eotaxin were significantly increased with the disease severity in the secondary 
      cohort patient group (n = 235). We also examined associations between cytokine 
      levels and clinical parameters, such as cytology and HPV genotype. Of the 14 
      cytokines, granulocyte colony-stimulating factor (G-CSF) was downregulated in 
      HPV-positive specimens. Examination of co-expression patterns of cytokines in 
      relation to HPV infection status revealed that several pairs of cytokines were 
      simultaneously upregulated in HPV-positive cases, including INF-gamma and interleukin 
      (IL)-17A, GM-CSF and monocyte chemoattractant protein-1 (MCP-1), GM-CSF and 
      RANTES, IL-17A and RANTES, and MCP-1 and eotaxin. Interestingly, upregulation of 
      GM-CSF and RANTES might reflect a shift in immuno-regulatory cytokines in 
      HPV-positive specimens, potentially associated with more severe cervical 
      neoplasia.
CI  - Copyright (c) 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Otani, Sayaka
AU  - Otani S
AD  - Department of Obstetrics and Gynecology, Fujita Health University, School of 
      Medicine, 1-98, Dengakugakubo, Kutsukakecho, Toyoake, Aichi 470-1192, Japan.
FAU - Fujii, Takuma
AU  - Fujii T
AD  - Department of Obstetrics and Gynecology, Fujita Health University, School of 
      Medicine, 1-98, Dengakugakubo, Kutsukakecho, Toyoake, Aichi 470-1192, Japan. 
      Electronic address: fujiit44@fujita-hu.ac.jp.
FAU - Kukimoto, Iwao
AU  - Kukimoto I
AD  - Pathogen Genomics Center, National Institute of Infectious Diseases, 4-7-1, 
      Gakuen, Musashi-murayama, Tokyo 208-0011, Japan.
FAU - Yamamoto, Naoki
AU  - Yamamoto N
AD  - Regenerative Medicine Support Promotion Facility, Center for Research Promotion 
      and Support, Fujita Health University, School of Medicine, 1-98, Dengakugakubo, 
      Kutsukakecho, Toyoake, Aichi 470-1192, Japan.
FAU - Tsukamoto, Tetsuya
AU  - Tsukamoto T
AD  - Department of Pathology, Fujita Health University, School of Medicine, 1-98, 
      Dengakugakubo, Kutsukakecho, Toyoake, Aichi 470-1192, Japan.
FAU - Ichikawa, Ryoko
AU  - Ichikawa R
AD  - Department of Obstetrics and Gynecology, Fujita Health University, School of 
      Medicine, 1-98, Dengakugakubo, Kutsukakecho, Toyoake, Aichi 470-1192, Japan.
FAU - Nishio, Eiji
AU  - Nishio E
AD  - Department of Obstetrics and Gynecology, Fujita Health University, School of 
      Medicine, 1-98, Dengakugakubo, Kutsukakecho, Toyoake, Aichi 470-1192, Japan.
FAU - Iwata, Aya
AU  - Iwata A
AD  - Department of Obstetrics and Gynecology, Fujita Health University, School of 
      Medicine, 1-98, Dengakugakubo, Kutsukakecho, Toyoake, Aichi 470-1192, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190520
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Cytokines)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cell Line, Tumor
MH  - Cervix Mucus/*metabolism
MH  - Cohort Studies
MH  - Cytokines/*metabolism
MH  - Female
MH  - Humans
MH  - Middle Aged
MH  - Papillomavirus Infections/metabolism/virology
MH  - Precancerous Conditions/*metabolism/virology
MH  - Severity of Illness Index
MH  - Uterine Cervical Neoplasms/*metabolism/virology
MH  - Young Adult
OTO - NOTNLM
OT  - Cervix
OT  - Chemokine
OT  - Immune response
OT  - Papillomavirus
EDAT- 2019/05/24 06:00
MHDA- 2020/08/19 06:00
CRDT- 2019/05/24 06:00
PHST- 2018/10/30 00:00 [received]
PHST- 2019/05/13 00:00 [revised]
PHST- 2019/05/13 00:00 [accepted]
PHST- 2019/05/24 06:00 [pubmed]
PHST- 2020/08/19 06:00 [medline]
PHST- 2019/05/24 06:00 [entrez]
AID - S1043-4666(19)30129-2 [pii]
AID - 10.1016/j.cyto.2019.05.011 [doi]
PST - ppublish
SO  - Cytokine. 2019 Aug;120:210-219. doi: 10.1016/j.cyto.2019.05.011. Epub 2019 May 
      20.