PMID- 31121922
OWN - NLM
STAT- MEDLINE
DCOM- 20191212
LR  - 20200225
IS  - 1648-9144 (Electronic)
IS  - 1010-660X (Print)
IS  - 1010-660X (Linking)
VI  - 55
IP  - 5
DP  - 2019 May 22
TI  - Lower Vitamin D Levels, but Not VDR Polymorphisms, Influence Type 2 Diabetes 
      Mellitus in Brazilian Population Independently of Obesity.
LID - 10.3390/medicina55050188 [doi]
LID - 188
AB  - Background and Objectives: Vitamin D levels have been associated with a diversity 
      of diseases, including obesity. Vitamin D presents a pleiotropic action, and can 
      regulate insulin secretion and inflammatory responses. Vitamin D receptor (VDR) 
      gene polymorphisms are involved in the gene expression regulation and have been 
      associated with type 2 diabetes mellitus (T2DM). This study aimed to evaluate the 
      association between the polymorphisms ApaI (rs7975232), BsmI (rs1544410), FokI 
      (rs10735810), and TaqI (rs731236) in the VDR gene in people diagnosed with T2DM, 
      and plasma 25-hydroxivitamin D levels [25(OH)D]. Materials and Methods: A total 
      of 101 T2DM patients and 62 gender, age, and body mass index (BMI) matched 
      non-diabetic controls were included in this study. Molecular analyzes were 
      performed by polymerase chain reaction-restriction fragment length polymorphism 
      (PCR-RFLP). The plasma 25(OH)D levels were measured by high performance liquid 
      chromatography. Results: The plasma 25(OH)D levels were lower in T2DM patients 
      (17.2 (16.6) ng/mL) when compared with the control subjects (30.8 (16.2) ng/mL, p 
      < 0.0001), independently of obesity status. We found no difference between 
      genotypic and allelic frequencies of the VDR polymorphisms when comparing the 
      T2DM group and control group (p > 0.05 for all), and did not show any association 
      with plasma 25(OH)D levels. Conclusions: These results suggest that T2DM is 
      associated with lower plasma 25(OH)D levels, which are not related to BMI and VDR 
      gene polymorphisms.
FAU - Rodrigues, Kathryna Fontana
AU  - Rodrigues KF
AD  - Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo 
      Horizonte, MG 31270-901, Brazil. katyfontanar@gmail.com.
FAU - Pietrani, Nathalia Teixeira
AU  - Pietrani NT
AD  - Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo 
      Horizonte, MG 31270-901, Brazil. ntpietrani@hotmail.com.
FAU - Bosco, Adriana Aparecida
AU  - Bosco AA
AD  - Instituto de Ensino e Pesquisa, Santa Casa de Belo Horizonte, Belo Horizonte, MG 
      30150-240, Brazil. a.bosco@terra.com.br.
FAU - de Sousa, Maira Candida Rodrigues
AU  - de Sousa MCR
AD  - Faculdade de Farmacia, Universidade Federal de Minas Gerais, Belo Horizonte, MG 
      31270-901, Brazil. maira.crsousa@gmail.com.
FAU - Silva, Ieda de Fatima Oliveira
AU  - Silva IFO
AD  - Faculdade de Farmacia, Universidade Federal de Minas Gerais, Belo Horizonte, MG 
      31270-901, Brazil. iedafos@farmacia.ufmg.br.
FAU - Silveira, Josianne Nicacio
AU  - Silveira JN
AD  - Faculdade de Farmacia, Universidade Federal de Minas Gerais, Belo Horizonte, MG 
      31270-901, Brazil. nicacio@farmacia.ufmg.br.
FAU - Gomes, Karina Braga
AU  - Gomes KB
AUID- ORCID: 0000-0002-6870-2063
AD  - Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo 
      Horizonte, MG 31270-901, Brazil. karinabgb@gmail.com.
AD  - Faculdade de Farmacia, Universidade Federal de Minas Gerais, Belo Horizonte, MG 
      31270-901, Brazil. karinabgb@gmail.com.
LA  - eng
GR  - -/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/
GR  - -/Fundacao de Amparo a Pesquisa do Estado de Minas Gerais/
PT  - Journal Article
DEP - 20190522
PL  - Switzerland
TA  - Medicina (Kaunas)
JT  - Medicina (Kaunas, Lithuania)
JID - 9425208
RN  - 0 (Blood Glucose)
RN  - 0 (Receptors, Calcitriol)
RN  - 0 (VDR protein, human)
RN  - 1406-16-2 (Vitamin D)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Blood Glucose/analysis
MH  - Brazil
MH  - Case-Control Studies
MH  - Diabetes Mellitus, Type 2/*blood/metabolism
MH  - Fasting/blood
MH  - Female
MH  - Humans
MH  - Insulin Resistance/physiology
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Genetic/*physiology
MH  - Receptors, Calcitriol/analysis/blood/*genetics
MH  - Statistics, Nonparametric
MH  - Vitamin D/analysis/blood
MH  - Vitamin D Deficiency/blood/*complications/*genetics
PMC - PMC6572088
OTO - NOTNLM
OT  - obesity
OT  - polymorphisms
OT  - type 2 diabetes mellitus
OT  - vitamin D
OT  - vitamin D receptor
COIS- The authors declare that they have no conflict of interest.
EDAT- 2019/05/28 06:00
MHDA- 2019/12/18 06:00
PMCR- 2019/05/22
CRDT- 2019/05/25 06:00
PHST- 2019/03/12 00:00 [received]
PHST- 2019/05/16 00:00 [revised]
PHST- 2019/05/17 00:00 [accepted]
PHST- 2019/05/25 06:00 [entrez]
PHST- 2019/05/28 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/05/22 00:00 [pmc-release]
AID - medicina55050188 [pii]
AID - medicina-55-00188 [pii]
AID - 10.3390/medicina55050188 [doi]
PST - epublish
SO  - Medicina (Kaunas). 2019 May 22;55(5):188. doi: 10.3390/medicina55050188.