PMID- 31122423
OWN - NLM
STAT- MEDLINE
DCOM- 20190617
LR  - 20191210
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 201
DP  - 2019 Aug 15
TI  - A novel aptamer-based online magnetic solid phase extraction method for 
      simultaneous determination of urinary 8-hydroxy-2'-deoxyguanosine and 
      monohydroxylated polycyclic aromatic hydrocarbons.
PG  - 271-279
LID - S0039-9140(19)30393-5 [pii]
LID - 10.1016/j.talanta.2019.04.004 [doi]
AB  - In this work, an innovative aptamer-based magnetic adsorbent 
      (Fe(3)O(4)@PDA-aptamer MNPs) was prepared by hydrothermal synthesis method 
      followed by the surface functionalization of nanoparticles. After fixing in a 
      steel stainless tube as sorbent of magnetic solid phase extraction (MSPE), an 
      online magnetic solid phase extraction-high performance liquid 
      chromatography-mass spectrometry (online-MSPE-HPLC-MS) method was developed and 
      applied for the determination of 8-hydroxy-2'-deoxyguanosine (8-OHdG) and 
      monohydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) simultaneously in 
      urine. The synthesized sorbent presented outstanding features, including large 
      specific surface area, high enrichment capacity and excellent stability. High 
      throughput analysis can be achieved by affinity-specific adsorption of 8-OHdG and 
      non-specific adsorption of OH-PAHs at the same time. In addition, online MSPE can 
      greatly simplify the analysis process, reduce human errors and enhance the 
      sensitivity. When compared with offline MSPE, a sensitivity enhancement of 30-400 
      times was obtained for the online method. Some experimental parameters such as 
      the amount of the sorbent, sampling flow rate and sample volume, were optimized 
      systematically. Under the optimal conditions, the limits of detection (LOD) were 
      in the range of 0.028-0.114 ng mL(-1), and the correlation coefficients (R(2)) 
      were higher than 0.9962. The relative standard deviations (RSDs) were less than 
      16.1% (n = 5) and the recoveries ranged from 71% to 116%. The above results show 
      that the rapid, sensitive and automated online-MSPE-HPLC-MS method has potential 
      application in the simultaneous determination of 8-OHdG and PAHs in complex 
      sample matrix to assess the environmental exposure level.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Gan, Haijiao
AU  - Gan H
AD  - Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, College of 
      Chemistry, Central China Normal University, Wuhan, China.
FAU - Xu, Hui
AU  - Xu H
AD  - Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, College of 
      Chemistry, Central China Normal University, Wuhan, China. Electronic address: 
      huixu@mail.ccnu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190406
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Magnetite Nanoparticles)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - 88847-89-6 (8-Hydroxy-2'-Deoxyguanosine)
RN  - G9481N71RO (Deoxyguanosine)
SB  - IM
MH  - 8-Hydroxy-2'-Deoxyguanosine
MH  - Adolescent
MH  - Adsorption
MH  - Adult
MH  - Aptamers, Nucleotide/*chemistry
MH  - Base Sequence
MH  - Chromatography, High Pressure Liquid
MH  - Deoxyguanosine/*analogs & derivatives/urine
MH  - Humans
MH  - Limit of Detection
MH  - Magnetite Nanoparticles/chemistry
MH  - Mass Spectrometry
MH  - Polycyclic Aromatic Hydrocarbons/*urine
MH  - Solid Phase Extraction/*methods
MH  - Young Adult
OTO - NOTNLM
OT  - 8-Hydroxy-2'-deoxyguanosine
OT  - Aptamer
OT  - High performance liquid chromatography-mass spectrometry
OT  - Online magnetic solid phase extraction
OT  - Polycyclic aromatic hydrocarbon
OT  - Urine
EDAT- 2019/05/28 06:00
MHDA- 2019/06/18 06:00
CRDT- 2019/05/25 06:00
PHST- 2018/11/06 00:00 [received]
PHST- 2019/03/05 00:00 [revised]
PHST- 2019/04/02 00:00 [accepted]
PHST- 2019/05/25 06:00 [entrez]
PHST- 2019/05/28 06:00 [pubmed]
PHST- 2019/06/18 06:00 [medline]
AID - S0039-9140(19)30393-5 [pii]
AID - 10.1016/j.talanta.2019.04.004 [doi]
PST - ppublish
SO  - Talanta. 2019 Aug 15;201:271-279. doi: 10.1016/j.talanta.2019.04.004. Epub 2019 
      Apr 6.