PMID- 31125355
OWN - NLM
STAT- MEDLINE
DCOM- 20200129
LR  - 20200309
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 14
IP  - 5
DP  - 2019
TI  - The impact of acute thermal stress on the metabolome of the black rockfish 
      (Sebastes schlegelii).
PG  - e0217133
LID - 10.1371/journal.pone.0217133 [doi]
LID - e0217133
AB  - Acute change in water temperature causes heavy economic losses in the aquaculture 
      industry. The present study investigated the metabolic and molecular effects of 
      acute thermal stress on black rockfish (Sebastes schlegelii). Gas chromatography 
      time-of-flight mass spectrometry (GC-TOF-MS)-based metabolomics was used to 
      investigate the global metabolic response of black rockfish at a high water 
      temperature (27 degrees C), low water temperature (5 degrees C) and normal water temperature 
      (16 degrees C). Metabolites involved in energy metabolism and basic amino acids were 
      significantly increased upon acute exposure to 27 degrees C (P < 0.05), and no change in 
      metabolite levels occurred in the low water temperature group. However, certain 
      fatty acid levels were elevated after cold stress (P < 0.05), and this effect was 
      not observed in the 27 degrees C group, suggesting that acute high and low temperature 
      exposures caused different physiological responses. Using quantitative real-time 
      PCR, we analyzed the expression of ubiquitin (ub), hypoxia-inducible factor 
      (hif), lactate dehydrogenase (ldh), and acetyl-CoA carboxylase (acac). Higher 
      expression levels of ub, hif, and ldh (P < 0.05) were observed in the high water 
      temperature group, but no changes in these expression levels occurred in the low 
      water temperature group. Our findings provide a potential metabolic profile for 
      black rockfish when exposed to acute temperature stress and provide some insights 
      into host metabolic and molecular responses to thermal stress.
FAU - Song, Min
AU  - Song M
AUID- ORCID: 0000-0003-2734-9328
AD  - Key Laboratory of Mariculture (Ocean University of China), Ministry of Education, 
      Ocean University of China, Qingdao, P. R. China.
FAU - Zhao, Ji
AU  - Zhao J
AD  - Key Laboratory of Mariculture (Ocean University of China), Ministry of Education, 
      Ocean University of China, Qingdao, P. R. China.
FAU - Wen, Hai-Shen
AU  - Wen HS
AD  - Key Laboratory of Mariculture (Ocean University of China), Ministry of Education, 
      Ocean University of China, Qingdao, P. R. China.
FAU - Li, Yun
AU  - Li Y
AD  - Key Laboratory of Mariculture (Ocean University of China), Ministry of Education, 
      Ocean University of China, Qingdao, P. R. China.
FAU - Li, Ji-Fang
AU  - Li JF
AD  - Key Laboratory of Mariculture (Ocean University of China), Ministry of Education, 
      Ocean University of China, Qingdao, P. R. China.
FAU - Li, Lan-Min
AU  - Li LM
AD  - Key Laboratory of Mariculture (Ocean University of China), Ministry of Education, 
      Ocean University of China, Qingdao, P. R. China.
FAU - Tao, Ya-Xiong
AU  - Tao YX
AD  - Department of Anatomy, Physiology and Pharmacology, College of Veterinary 
      Medicine, Auburn University, Auburn, AL, United States of America.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190524
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Animals
MH  - *Heat-Shock Response
MH  - *Metabolome
MH  - Metabolomics
MH  - Perciformes/*metabolism
PMC - PMC6534312
COIS- The authors have declared that no competing interests exist.
EDAT- 2019/05/28 06:00
MHDA- 2020/01/30 06:00
PMCR- 2019/05/24
CRDT- 2019/05/25 06:00
PHST- 2018/08/26 00:00 [received]
PHST- 2019/05/06 00:00 [accepted]
PHST- 2019/05/25 06:00 [entrez]
PHST- 2019/05/28 06:00 [pubmed]
PHST- 2020/01/30 06:00 [medline]
PHST- 2019/05/24 00:00 [pmc-release]
AID - PONE-D-18-23377 [pii]
AID - 10.1371/journal.pone.0217133 [doi]
PST - epublish
SO  - PLoS One. 2019 May 24;14(5):e0217133. doi: 10.1371/journal.pone.0217133. 
      eCollection 2019.