PMID- 31127661
OWN - NLM
STAT- MEDLINE
DCOM- 20200914
LR  - 20200914
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 120
IP  - 10
DP  - 2019 Oct
TI  - Comprehensive analysis of differentially expressed profiles of lncRNAs, mRNAs, 
      and miRNAs in laryngeal squamous cell carcinoma in order to construct a ceRNA 
      network and identify potential biomarkers.
PG  - 17963-17974
LID - 10.1002/jcb.29063 [doi]
AB  - OBJECTIVE: This study aimed to uncover a regulatory network comprised of long 
      noncoding RNAs (lncRNAs), microRNAs (miRNAs), and messenger RNAs (mRNAs) in 
      laryngeal squamous cell carcinoma (LSCC), to explore its underlying mechanisms 
      and development, and to identify key genetic biomarkers for the prognosis of 
      LSCC. METHODS: Here, we compared mRNA, lncRNA, and miRNA expression profiles 
      between 111 LSCC and 12 adjacent normal tissues using RNA sequencing (RNA-Seq) 
      data from the Cancer Genome Atlas (TCGA) database. Based on the interaction 
      information obtained from miRcode, TargetScan, miRTarBase, and miRDB, a 
      lncRNA-miRNA-mRNA competing endogenous RNA (ceRNA) network was constructed using 
      differentially expressed lncRNAs (DElncRNA), miRNAs (DEmiRNA), and mRNAs 
      (DEmRNA). By assessing the functional enrichment of DEmRNAs in this network, the 
      potential underlying mechanisms were explored. In addition, Kaplan-Meier survival 
      analysis was used to assess genetic biomarkers related to the prognosis of LSCC 
      patients. RESULTS: Upon comparing LSCC and control tissues, 1640 DElncRNAs, 75 
      DEmiRNAs, and 3217 DEmRNAs were identified. Based on the prediction between 
      lncRNA-miRNA and miRNA-mRNA relationships, we constructed a ceRNA network 
      comprised of 93 lncRNAs, nine miRNAs, and nine mRNAs. This network predicted that 
      two lncRNAs (AC016773.1 and C00299), two mRNAs (DIO1 and STC2), and two miRNAs 
      (hsa-mir-137 and hsa-mir-210) were significant biomarkers of LSCC prognosis 
      according to thorough topological and survival analyses (P < .05). CONCLUSION: 
      Through a ceRNA network analysis, our study identifies new lncRNAs, miRNAs, and 
      mRNAs, which can be used as potential biomarkers of LSCC and as therapeutic 
      targets for treating LSCC, thus laying a foundation for future clinical studies.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Kong, Xinru
AU  - Kong X
AD  - Clinical Medical College, Weifang Medical University, Weifang, P.R. China.
FAU - Qi, Jixia
AU  - Qi J
AD  - Clinical Medical College, Weifang Medical University, Weifang, P.R. China.
FAU - Yan, Yao
AU  - Yan Y
AD  - Clinical Medical College, Weifang Medical University, Weifang, P.R. China.
FAU - Chen, Liwei
AU  - Chen L
AD  - Hainan Hospital of Chinese PLA General Hospital, Sanya, P.R. China.
FAU - Zhao, Yali
AU  - Zhao Y
AD  - Central Lab, Hainan Hospital of Chinese PLA General Hospital, Sanya, P.R. China.
FAU - Fang, Zhongju
AU  - Fang Z
AD  - Clinical Medical College, Weifang Medical University, Weifang, P.R. China.
FAU - Fan, Junda
AU  - Fan J
AD  - Hainan Hospital of Chinese PLA General Hospital, Sanya, P.R. China.
FAU - Liu, Mingbo
AU  - Liu M
AUID- ORCID: 0000-0002-0540-2573
AD  - Hainan Hospital of Chinese PLA General Hospital, Sanya, P.R. China.
AD  - Clinical Medical Research Center for Otolaryngology Head and Neck Diseases of 
      Hainan Province, Sanya, P.R. China.
FAU - Liu, Yehai
AU  - Liu Y
AD  - Department of Otorhinolaryngology, Head & Neck Surgery, The First Affiliated 
      Hospital of Anhui Medical University, Hefei, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190524
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Biomarkers, Tumor/*genetics/metabolism
MH  - Carcinoma, Squamous Cell/*genetics
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation, Neoplastic
MH  - Gene Ontology
MH  - *Gene Regulatory Networks
MH  - Humans
MH  - Kaplan-Meier Estimate
MH  - Laryngeal Neoplasms/*genetics
MH  - MicroRNAs/*genetics/metabolism
MH  - Molecular Sequence Annotation
MH  - RNA, Long Noncoding/*genetics/metabolism
MH  - RNA, Messenger/*genetics
OTO - NOTNLM
OT  - LSCC
OT  - biomarkers
OT  - ceRNA network
OT  - expression profiles
OT  - overall survival
EDAT- 2019/05/28 06:00
MHDA- 2020/09/15 06:00
CRDT- 2019/05/26 06:00
PHST- 2019/02/21 00:00 [received]
PHST- 2019/04/18 00:00 [revised]
PHST- 2019/04/29 00:00 [accepted]
PHST- 2019/05/28 06:00 [pubmed]
PHST- 2020/09/15 06:00 [medline]
PHST- 2019/05/26 06:00 [entrez]
AID - 10.1002/jcb.29063 [doi]
PST - ppublish
SO  - J Cell Biochem. 2019 Oct;120(10):17963-17974. doi: 10.1002/jcb.29063. Epub 2019 
      May 24.