PMID- 31128004
OWN - NLM
STAT- MEDLINE
DCOM- 20191227
LR  - 20191227
IS  - 2241-6293 (Electronic)
IS  - 1107-0625 (Linking)
VI  - 24
IP  - 2
DP  - 2019 Mar-Apr
TI  - Root extract of Prunella vulgaris inhibits in vitro and in vivo carcinogenesis in 
      MCF-5 human breast carcinoma via suppression of angiogenesis, induction of 
      apoptosis, cell cycle arrest and modulation of PI3K/AKT signalling pathway.
PG  - 549-554
AB  - PURPOSE: In this study we examined the anticancer effects of methanolic root 
      extract of Prunella Vulgaris (PVE) against the MCF-5 breast cancer (BC) cell line 
      along with its mode of action. METHODS: The proliferation rate of the MCF-5 cells 
      was assessed by MTT assay. Apoptosis was confirmed by acridine orange 
      (AO)/ethidium bromide (EB) and annexin V/propidium iodide (PI) staining. DNA 
      damage was checked by comet assay. Cell cycle analysis was performed by flow 
      cytometry. Protein expression was determined by western blotting. In vivo 
      evaluation of the extract was carried out in xenografted tumor mice models. 
      RESULTS: PVE inhibited the growth of the MCF-5 cells and exhibited an IC50 value 
      of 25 microg/ml. The investigation of underlying mechanism revealed that PVE 
      triggered apoptotic cell death of the MCF-5 cells which was also associated with 
      enhancement of the expression of Bax and decrease in the expression of Bcl-2. PVE 
      also caused arrest of the cells in the G2/M phase of the cell cycle and also 
      exerted the anti-angiogenic effects. In vivo evaluation of PVE showed that it 
      could inhibit the tumor weight and volume, suggestive of the anticancer potential 
      of PVE. CONCLUSION: The root extract of Prunella vulgaris in this study was shown 
      to exert potent anticancer effects in MCF-7 human BC cells both in vitro and in 
      vivo, accompanied with apoptosis induction, inhibition of angiogenesis, cell 
      cycle arrest, and modulation of PI3K/AKT signaling pathway.
FAU - Gao, Wei
AU  - Gao W
AD  - The Department of Modern and Traditional Medicine, The Tumor Hospital of Qingdao, 
      Qingdao, 266000, China.
FAU - Xu, Hua Liang Yanlei Li Yuanyuan Liu Yanxia
AU  - Xu HLYLYLY
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 0 (Plant Extracts)
RN  - EC 2.7.11.1 (Oncogene Protein v-akt)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Breast Neoplasms/*drug therapy/genetics/pathology
MH  - Cell Cycle Checkpoints/drug effects
MH  - Cell Proliferation/*drug effects
MH  - Female
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Humans
MH  - Mice
MH  - Neovascularization, Pathologic/*drug therapy/genetics/pathology
MH  - Oncogene Protein v-akt/genetics
MH  - Phosphatidylinositol 3-Kinases/genetics
MH  - Plant Extracts/chemistry/pharmacology
MH  - Plant Roots/chemistry
MH  - Prunella/*chemistry
MH  - Xenograft Model Antitumor Assays
EDAT- 2019/05/28 06:00
MHDA- 2019/12/28 06:00
CRDT- 2019/05/26 06:00
PHST- 2019/05/26 06:00 [entrez]
PHST- 2019/05/28 06:00 [pubmed]
PHST- 2019/12/28 06:00 [medline]
PST - ppublish
SO  - J BUON. 2019 Mar-Apr;24(2):549-554.