PMID- 31128514
OWN - NLM
STAT- MEDLINE
DCOM- 20200116
LR  - 20200116
IS  - 1873-4367 (Electronic)
IS  - 0927-7765 (Linking)
VI  - 181
DP  - 2019 Sep 1
TI  - Dual-antibody Modified PLGA Nanofibers for Speci fi c Capture of Epithelial and 
      Mesenchymal CTCs.
PG  - 143-148
LID - S0927-7765(19)30339-X [pii]
LID - 10.1016/j.colsurfb.2019.05.031 [doi]
AB  - CTC detection has great potential to provide crucial clinical information for 
      early cancer diagnosis, patient prognosis, personalized therapy, and cancer 
      progress monitoring etc. It has been proved that the disease progress is 
      associated with an increase in mesenchymal CTCs, while most mature techniques 
      have been developed based on epithelial CTC detection. In present work, a 
      dual-antibody nanointerface against EpCAM and N-Cadherin was developed to capture 
      epithelial CTCs as well as mesenchymal CTCs from blood samples. A uniform 
      poly-(lactic-co-glycolic acid) (PLGA) nanofiber substrate was fabricated by 
      electrospinning to provide a platform for cell capture (i.e., MCF-7 and GIST882 
      cells), modified with BSA and dual antibodies. Our results showed the 
      dual-antibody substrates exhibited an improved capture efficiency of target cells 
      compared to the mono-antibody ones, revealing potential application of the 
      dual-antibody PLGA nanofibers for e ffi cient capture of epithelial and mesenchymal 
      CTCs in clinic.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Liu, Hui
AU  - Liu H
AD  - CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and 
      Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China; Nano Science 
      and Technology Institute, University of Science and Technology of China, Suzhou, 
      215123, China.
FAU - Wang, Zhili
AU  - Wang Z
AD  - CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and 
      Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
FAU - Chen, Changchong
AU  - Chen C
AD  - CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and 
      Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China; Nano Science 
      and Technology Institute, University of Science and Technology of China, Suzhou, 
      215123, China.
FAU - Ding, Pi
AU  - Ding P
AD  - CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and 
      Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China.
FAU - Sun, Na
AU  - Sun N
AD  - CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and 
      Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China. Electronic 
      address: nsun2013@sinano.ac.cn.
FAU - Pei, Renjun
AU  - Pei R
AD  - CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and 
      Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China. Electronic 
      address: rjpei2011@sinano.ac.cn.
LA  - eng
PT  - Journal Article
DEP - 20190515
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Antibodies)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
SB  - IM
MH  - Antibodies/*chemistry
MH  - Cell Line, Tumor
MH  - *Cell Separation
MH  - Humans
MH  - MCF-7 Cells
MH  - Nanofibers/*chemistry
MH  - Neoplastic Cells, Circulating/*pathology
MH  - Particle Size
MH  - Polylactic Acid-Polyglycolic Acid Copolymer/*chemistry
MH  - Surface Properties
OTO - NOTNLM
OT  - circulating tumor cells (CTCs)
OT  - dual-antibody
OT  - epithelial CTCs
OT  - mesenchymal CTCs
OT  - nanofiber interface
EDAT- 2019/05/28 06:00
MHDA- 2020/01/17 06:00
CRDT- 2019/05/26 06:00
PHST- 2018/11/20 00:00 [received]
PHST- 2019/03/13 00:00 [revised]
PHST- 2019/05/14 00:00 [accepted]
PHST- 2019/05/28 06:00 [pubmed]
PHST- 2020/01/17 06:00 [medline]
PHST- 2019/05/26 06:00 [entrez]
AID - S0927-7765(19)30339-X [pii]
AID - 10.1016/j.colsurfb.2019.05.031 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2019 Sep 1;181:143-148. doi: 
      10.1016/j.colsurfb.2019.05.031. Epub 2019 May 15.