PMID- 31129926
OWN - NLM
STAT- MEDLINE
DCOM- 20200421
LR  - 20210109
IS  - 2045-8827 (Electronic)
IS  - 2045-8827 (Linking)
VI  - 8
IP  - 9
DP  - 2019 Sep
TI  - Biogas-producing microbial composition of an anaerobic digester and associated 
      bovine residues.
PG  - e00854
LID - 10.1002/mbo3.854 [doi]
LID - e00854
AB  - Influenced by feedstock type and microbial inoculum, different microbial groups 
      must precisely interact for high-quality biogas yields. As a first approach for 
      optimization, this study aimed to identify through time the biogas-producing 
      microbial community in a 10-ton dry anaerobic digester treating cattle manure by 
      denaturing gradient gel electrophoresis (DGGE) and metagenomics. Moreover, the 
      associated bovine residues or feedstocks (leachate, manure, oxidation lagoon 
      water, rumen) were also characterized to determine their contribution. A diverse 
      and dynamic community characterized by Bacteria (82%-88%) and a considerable 
      amount of Archaea (8%-15%) presented profiles particular to each stage of biogas 
      production. Eukaryotes (2.6%-3.6%), mainly fungi, were a minor but stable 
      component. Proteobacteria represented 47% of the community at the start of the 
      run but only 18% at the end, opposite to the Bacteroidetes/Chlorobi group (8% and 
      20%, respectively), while Firmicutes (12%-18%) and Actinobacteria (12%-32%) 
      remained relatively constant. Methanogens of the order Methanomicrobiales 
      represented by several species of Methanoculleus were abundant at the end of the 
      run (77%) contrary to Methanosarcinales (11%) and Methanobacteriales (0.7%). 
      Therefore, methanogenesis mainly occurred by the hydrogenotrophic pathway. Manure 
      and oxidation lagoon water seemed to contribute key microorganisms, while rumen 
      dominated by Methanobrevibacter (72%) did not proliferate in the digester. Manure 
      particularly possessed Methanoculleus (24%) and uncultured methanogens identified 
      by DGGE, whereas oxidation lagoon was exclusively abundant in Methanolinea (18%) 
      and Methanosaeta (19%). Leachate, as the microbial inoculum from a previous run, 
      adequately preserved the biogas-producing community. These results could lead to 
      higher biogas yields through bioaugmentation strategies by incorporating higher 
      proportions or an enriched inoculum from the relevant feedstocks.
CI  - (c) 2019 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
FAU - Senes-Guerrero, Carolina
AU  - Senes-Guerrero C
AD  - Tecnologico de Monterrey, Escuela de Ingenieria y Ciencias, Centro de 
      Biotecnologia-FEMSA, Monterrey, Mexico.
FAU - Colon-Contreras, Franco A
AU  - Colon-Contreras FA
AD  - Tecnologico de Monterrey, Escuela de Ingenieria y Ciencias, Centro de 
      Biotecnologia-FEMSA, Monterrey, Mexico.
FAU - Reynoso-Lobo, Javier F
AU  - Reynoso-Lobo JF
AD  - SuKarne, Culiacan, Mexico.
FAU - Tinoco-Perez, Benito
AU  - Tinoco-Perez B
AD  - SuKarne, Culiacan, Mexico.
FAU - Siller-Cepeda, Jorge H
AU  - Siller-Cepeda JH
AD  - SuKarne, Culiacan, Mexico.
AD  - Secretaria de Agricultura y Ganaderia, Culiacan Rosales, Mexico.
FAU - Pacheco, Adriana
AU  - Pacheco A
AUID- ORCID: 0000-0002-9512-7674
AD  - Tecnologico de Monterrey, Escuela de Ingenieria y Ciencias, Centro de 
      Biotecnologia-FEMSA, Monterrey, Mexico.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190525
PL  - England
TA  - Microbiologyopen
JT  - MicrobiologyOpen
JID - 101588314
RN  - 0 (Biofuels)
RN  - 0 (Manure)
SB  - IM
MH  - Anaerobiosis
MH  - Animals
MH  - Archaea/*classification/genetics
MH  - Bacteria/*classification/genetics
MH  - Biofuels/*microbiology
MH  - *Biota
MH  - Cattle
MH  - Denaturing Gradient Gel Electrophoresis
MH  - Fungi/classification/genetics
MH  - Manure/*microbiology
MH  - Metagenomics
PMC - PMC6741126
OTO - NOTNLM
OT  - DGGE
OT  - anaerobic digestion
OT  - biogas
OT  - bovine residues
OT  - metagenomics
OT  - methanogens
COIS- The authors declare no conflict of interest.
EDAT- 2019/05/28 06:00
MHDA- 2020/04/22 06:00
PMCR- 2019/05/25
CRDT- 2019/05/27 06:00
PHST- 2019/02/16 00:00 [received]
PHST- 2019/04/01 00:00 [revised]
PHST- 2019/04/15 00:00 [accepted]
PHST- 2019/05/28 06:00 [pubmed]
PHST- 2020/04/22 06:00 [medline]
PHST- 2019/05/27 06:00 [entrez]
PHST- 2019/05/25 00:00 [pmc-release]
AID - MBO3854 [pii]
AID - 10.1002/mbo3.854 [doi]
PST - ppublish
SO  - Microbiologyopen. 2019 Sep;8(9):e00854. doi: 10.1002/mbo3.854. Epub 2019 May 25.