PMID- 31130951
OWN - NLM
STAT- MEDLINE
DCOM- 20201019
LR  - 20201019
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 10
DP  - 2019
TI  - Macrophage-B Cell Interactions in the Inverted Porcine Lymph Node and Their 
      Response to Porcine Reproductive and Respiratory Syndrome Virus.
PG  - 953
LID - 10.3389/fimmu.2019.00953 [doi]
LID - 953
AB  - Swine lymph nodes (LN) present an inverted structure compared to mouse and human, 
      with the afferent lymph diffusing from the center to the periphery. This 
      structure, also observed in close and distant species such as dolphins, 
      hippopotamus, rhinoceros, and elephants, is poorly described, nor are the LN 
      macrophage populations and their relationship with B cell follicles. B cell 
      maturation occurs mainly in LN B cell follicles with the help of LN macrophage 
      populations endowed with different antigen delivery capacities. We identified 
      three macrophage populations that we localized in the inverted LN spatial 
      organization. This allowed us to ascribe porcine LN MPhi to their murine 
      counterparts: subcapsular sinus MPhi, medullary cord MPhi and medullary sinus MPhi. We 
      identified the different intra and extrafollicular stages of LN B cells 
      maturation and explored the interaction of MPhi, drained antigen and follicular B 
      cells. The porcine reproductive and respiratory syndrome virus (PRRSV) is a major 
      porcine pathogen that infects tissue macrophages (MPhi). PRRSV is persistent in the 
      secondary lymphoid tissues and induces a delay in neutralizing antibodies 
      appearance. We observed PRRSV interaction with two LN MPhi populations, of which 
      one interacts closely with centroblasts. We observed BCL6 up-regulation in 
      centroblast upon PRRSV infection, leading to new hypothesis on PRRSV inhibition 
      of B cell maturation. This seminal study of porcine LN will permit fruitful 
      comparison with murine and human LN for a better understanding of normal and 
      inverted LN development and functioning.
FAU - Bordet, Elise
AU  - Bordet E
AD  - VIM-INRA-Universite Paris-Saclay, Domaine de Vilvert, Jouy-en-Josas, France.
FAU - Fretaud, Maxence
AU  - Fretaud M
AD  - VIM-INRA-Universite Paris-Saclay, Domaine de Vilvert, Jouy-en-Josas, France.
AD  - INRA, EMERG'IN- Plateforme d'Infectiologie Experimentale IERP- Domaine de 
      Vilvert, Jouy-en-Josas, France.
FAU - Crisci, Elisa
AU  - Crisci E
AD  - VIM-INRA-Universite Paris-Saclay, Domaine de Vilvert, Jouy-en-Josas, France.
AD  - UMR Genetique Animale et Biologie Integrative, INRA, AgroParisTech, Universite 
      Paris-Saclay, Jouy-en-Josas, France.
AD  - Department of Population Health and Pathobiology, College of Veterinary Medicine, 
      North Carolina State University, Raleigh, NC, United States.
FAU - Bouguyon, Edwige
AU  - Bouguyon E
AD  - VIM-INRA-Universite Paris-Saclay, Domaine de Vilvert, Jouy-en-Josas, France.
FAU - Rault, Stephane
AU  - Rault S
AD  - VIM-INRA-Universite Paris-Saclay, Domaine de Vilvert, Jouy-en-Josas, France.
FAU - Pezant, Jeremy
AU  - Pezant J
AD  - INRA, UE1277, Plate-Forme d'Infectiologie Experimentale, PFIE, Nouzilly, France.
FAU - Pleau, Alexis
AU  - Pleau A
AD  - INRA, UE1277, Plate-Forme d'Infectiologie Experimentale, PFIE, Nouzilly, France.
FAU - Renson, Patricia
AU  - Renson P
AD  - Anses, Laboratoire de Ploufragan-Plouzane-Niort, Unite Virologie et Immunologie 
      Porcines, Zoopole, BP53, Ploufragan, France.
AD  - Universite Bretagne Loire, Cite Internationale, Rennes, France.
FAU - Giuffra, Elisabetta
AU  - Giuffra E
AD  - UMR Genetique Animale et Biologie Integrative, INRA, AgroParisTech, Universite 
      Paris-Saclay, Jouy-en-Josas, France.
FAU - Larcher, Thibaut
AU  - Larcher T
AD  - INRA, UMR 703 APEX, Oniris, Nantes, France.
FAU - Bourge, Mickael
AU  - Bourge M
AD  - I2BC, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
FAU - Bourry, Olivier
AU  - Bourry O
AD  - Anses, Laboratoire de Ploufragan-Plouzane-Niort, Unite Virologie et Immunologie 
      Porcines, Zoopole, BP53, Ploufragan, France.
AD  - Universite Bretagne Loire, Cite Internationale, Rennes, France.
FAU - Boulesteix, Olivier
AU  - Boulesteix O
AD  - INRA, UE1277, Plate-Forme d'Infectiologie Experimentale, PFIE, Nouzilly, France.
FAU - Langevin, Christelle
AU  - Langevin C
AD  - VIM-INRA-Universite Paris-Saclay, Domaine de Vilvert, Jouy-en-Josas, France.
AD  - INRA, EMERG'IN- Plateforme d'Infectiologie Experimentale IERP- Domaine de 
      Vilvert, Jouy-en-Josas, France.
FAU - Schwartz-Cornil, Isabelle
AU  - Schwartz-Cornil I
AD  - VIM-INRA-Universite Paris-Saclay, Domaine de Vilvert, Jouy-en-Josas, France.
FAU - Bertho, Nicolas
AU  - Bertho N
AD  - VIM-INRA-Universite Paris-Saclay, Domaine de Vilvert, Jouy-en-Josas, France.
AD  - BIOEPAR, INRA, Oniris, Nantes, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190503
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/*immunology
MH  - Lymph Nodes/*immunology
MH  - Macrophages/*immunology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology
MH  - Porcine respiratory and reproductive syndrome virus
MH  - Swine
PMC - PMC6510060
OTO - NOTNLM
OT  - B cell
OT  - BCL6
OT  - PRRSV
OT  - centrocyte
OT  - lymph node
OT  - macrophage
OT  - swine
EDAT- 2019/05/28 06:00
MHDA- 2020/10/21 06:00
PMCR- 2019/01/01
CRDT- 2019/05/28 06:00
PHST- 2019/01/15 00:00 [received]
PHST- 2019/04/12 00:00 [accepted]
PHST- 2019/05/28 06:00 [entrez]
PHST- 2019/05/28 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2019.00953 [doi]
PST - epublish
SO  - Front Immunol. 2019 May 3;10:953. doi: 10.3389/fimmu.2019.00953. eCollection 
      2019.