PMID- 31132188
OWN - NLM
STAT- MEDLINE
DCOM- 20200109
LR  - 20211204
IS  - 1600-0714 (Electronic)
IS  - 0904-2512 (Linking)
VI  - 48
IP  - 8
DP  - 2019 Sep
TI  - Simultaneous activation of impaired autophagy and the mammalian target of 
      rapamycin pathway in oral squamous cell carcinoma.
PG  - 705-711
LID - 10.1111/jop.12884 [doi]
AB  - BACKGROUND: The aim of this study was to conduct a comparative evaluation of the 
      expression levels of autophagy markers and proteins of the mammalian target of 
      rapamycin (mTOR) pathway in normal, margin and tumour tissues of patients with 
      oral squamous cell carcinoma (OSCC). MATERIALS AND METHODS: Three regional 
      specimens, including normal, margin and tumour tissues, were collected from 26 
      patients with OSCC. Western blotting, immunohistochemistry and immunofluorescence 
      staining were performed to detect mTOR, eukaryotic translation initiation factor 
      4E-binding protein 1 (4E-BP1), p70 ribosomal S6 protein kinase (p70S6K) and the 
      corresponding phosphorylated proteins, as well as the light chain 3 (LC3) and 
      sequestosome-1 (SQSTM1, also known as p62) autophagy indicators. RESULTS: LC3-II, 
      p62, mTOR, phospho-mTOR, 4E-BP1 and phospho-4E-BP1 were highly expressed in the 
      margin and tumour groups. There were positive correlations between 
      mTOR/phospho-mTOR, mTOR/4E-BP1, mTOR/phospho-4E-BP1, mTOR/p70S6K, LC3-II/p62, 
      LC3-II/p70S6K, p62/4E-BP1 and p62/phospho-4E-BP1 in normal group, while 
      LC3-II/p62, LC3-II/mTOR, LC3-II/4E-BP1, LC3-II/phospho-4E-BP1, 
      phospho-4E-BP1/mTOR, phospho-4E-BP1/4E-BP1 and p62/4E-BP1 showed positive 
      relationships in margin group; however, in tumour group, only mTOR/phospho-mTOR, 
      4E-BP1/phospho-4E-BP1 and phospho-mTOR/p70S6K showed positive correlations. 
      CONCLUSION: The study suggests that autophagy is impaired in patients with OSCC 
      and impaired autophagy and the mTOR pathway are simultaneously activated in OSCC 
      cells.
CI  - (c) 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Yin, Xubin
AU  - Yin X
AD  - Salivary Gland Disease Center and Beijing Key Laboratory of Tooth Regeneration 
      and Function Reconstruction, Capital Medical University School of Stomatology, 
      Beijing, China.
AD  - Beijing Tian Tan Hospital, Capital Medical University, Beijing, China.
FAU - Hu, Liang
AU  - Hu L
AD  - Salivary Gland Disease Center and Beijing Key Laboratory of Tooth Regeneration 
      and Function Reconstruction, Capital Medical University School of Stomatology, 
      Beijing, China.
FAU - Feng, Xiaoyu
AU  - Feng X
AD  - Salivary Gland Disease Center and Beijing Key Laboratory of Tooth Regeneration 
      and Function Reconstruction, Capital Medical University School of Stomatology, 
      Beijing, China.
FAU - Wang, Hongyun
AU  - Wang H
AD  - Beijing Neurosurgical Institute, Capital Medical University, Beijing, China.
FAU - Zhang, Chunmei
AU  - Zhang C
AD  - Salivary Gland Disease Center and Beijing Key Laboratory of Tooth Regeneration 
      and Function Reconstruction, Capital Medical University School of Stomatology, 
      Beijing, China.
FAU - Wang, Hao
AU  - Wang H
AD  - Beijing Tian Tan Hospital, Capital Medical University, Beijing, China.
FAU - Wang, Songlin
AU  - Wang S
AUID- ORCID: 0000-0002-7066-2654
AD  - Salivary Gland Disease Center and Beijing Key Laboratory of Tooth Regeneration 
      and Function Reconstruction, Capital Medical University School of Stomatology, 
      Beijing, China.
AD  - Department of Biochemistry and Molecular Biology, Capital Medical University 
      School of Basic Medical Sciences, Beijing, China.
LA  - eng
GR  - Beijing Scholar Program-PXM2018_014226_000021/Beijing Municipality Government/
GR  - PXM2017_014226_000023/Beijing Municipality Government/
GR  - PXM2018_193312_000006_0028S643_FCG/Beijing Municipality Government/
GR  - 91649124/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20190617
PL  - Denmark
TA  - J Oral Pathol Med
JT  - Journal of oral pathology & medicine : official publication of the International 
      Association of Oral Pathologists and the American Academy of Oral Pathology
JID - 8911934
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (EIF4EBP1 protein, human)
RN  - 0 (MAP1LC3A protein, human)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (SQSTM1 protein, human)
RN  - 0 (Sequestosome-1 Protein)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/metabolism
MH  - Aged
MH  - *Autophagy
MH  - Carcinoma, Squamous Cell/*metabolism/pathology
MH  - Cell Cycle Proteins/metabolism
MH  - Female
MH  - Humans
MH  - Male
MH  - Microtubule-Associated Proteins/metabolism
MH  - Middle Aged
MH  - Mouth Neoplasms/*metabolism/pathology
MH  - Phosphoproteins
MH  - Phosphorylation
MH  - Ribosomal Protein S6 Kinases, 70-kDa/metabolism
MH  - Sequestosome-1 Protein/metabolism
MH  - *Signal Transduction
MH  - TOR Serine-Threonine Kinases/*metabolism
OTO - NOTNLM
OT  - autophagy
OT  - light chain 3
OT  - mammalian target of rapamycin
OT  - oral squamous cell carcinoma
OT  - p62
EDAT- 2019/05/28 06:00
MHDA- 2020/01/10 06:00
CRDT- 2019/05/28 06:00
PHST- 2019/05/10 00:00 [received]
PHST- 2019/05/11 00:00 [accepted]
PHST- 2019/05/28 06:00 [pubmed]
PHST- 2020/01/10 06:00 [medline]
PHST- 2019/05/28 06:00 [entrez]
AID - 10.1111/jop.12884 [doi]
PST - ppublish
SO  - J Oral Pathol Med. 2019 Sep;48(8):705-711. doi: 10.1111/jop.12884. Epub 2019 Jun 
      17.