PMID- 31133092
OWN - NLM
STAT- MEDLINE
DCOM- 20190829
LR  - 20190829
IS  - 1000-8020 (Print)
IS  - 1000-8020 (Linking)
VI  - 48
IP  - 2
DP  - 2019 Mar
TI  - [gamma-aminobutyric acid fortified rice alleviated oxidative stress and pancreatic 
      injury in type 2 diabetic mice].
PG  - 179-199
AB  - OBJECTIVE: To investigate the effects of gamma aminobutyric acid(GABA) fortified 
      rice diet intervention on oxidative stress and pancreatic injury in type 2 
      diabetes mellitus(T2 DM) mice. METHODS: Of the 70 male ICR mice, 10 were randomly 
      selected as blank control group and they were always fed with the normal white 
      rice feed. The remaining 60 mice were fed with high-fat white rice for 9 weeks. 
      They were fasted for 12 h and injected intraperitoneally with streptozocin(STZ) 
      at a dose of 50 mg/kg body weigh for two consecutive days. The control group was 
      injected with the corresponding volume of normal saline. Subsequently, 50 T2 DM 
      mice with successful modeling were randomly divided into 5 groups according to 
      blood glucose, 10 in each group: T2 DM model control group, germinated brown rice 
      positive control group(GABA content is 0. 2 g/kg feed), GABA-fortified rice low, 
      medium and high dose group(GABA content was 0. 02, 0. 1 and 0. 2 g/kg feed 
      respectively) and each target diet was fed for 6 weeks. Oral glucose tolerance 
      test was performed one week before the end of the experiment to observe the 
      hypoglycemic effect of different doses of GABA fortified rice. After the end of 
      the experiment, HE staining was used to observe the morphology of pancreas. At 
      the same time, the redox indicators from plasma and pancreas of reactive oxygen 
      species(ROS), malondialdehyde(MDA), total antioxidant capacity(T-AOC), 
      glutathione peroxidase(GSH-Px), superoxide dismutase(SOD) were examined in each 
      group; The mRNA expressions of oxidative stress-related genes including glycogen 
      synthase kinase-3beta(GSK-3beta), nuclear transcription factor 2(Nrf2), heme oxygenase 
      1(HO-1) and NAD(P)H: quinone oxidoreductase1(NQO1), insulin secretion related 
      genes including pancreatic and duodenal homeobox 1(PDX-1), mus musculus v-maf 
      musculoaponeurotic fibrosarcoma oncogene family, protein A(MafA), 
      glucokinase(GCK), glucose transporter 2(GLUT2) and the apoptosis associated genes 
      including b-cell lymphoma-2(Bcl-2), Bcl-2-associated X protein(Bax) and caspase-3 
      in pancreas were assayed by real-time fluorescent quantitative PCR. RESULTS: The 
      intervention of GABA fortified rice could alleviate the improvement of the blood 
      glucose level and the lack of insulin secretion in T2 DM mice and relieve plasma 
      and pancreatic oxidative stress. besides, The intervention of GABA fortified rice 
      could up-regulate the expression of insulin secretion-related genes PDX-1, GCK, 
      GLUT2, inhibit the expression of pro-apoptotic gene caspase-3 and promote the 
      expression of anti-apoptosis gene Bcl-2. There was a dose-response relationship 
      between the above result and the 0. 2 g/kg dose group was the most significant, 
      which achieved similar result to germinated brown rice. CONCLUSION: 
      GABA-fortified rice can significantly improve the plasma and pancreatic redox 
      status of STZ-induced T2 DM mice, regulate the expression levels of oxidative 
      stress-related genes and apoptosis-related genes, thereby protect pancreatic 
      tissue morphology, improve pancreatic insulin secretion and thereby alleviate 
      glucose metabolism.
FAU - Gao, Qiuli
AU  - Gao Q
AD  - State Key Laboratory of Food Science & Technology, Jiangnan University, Wuxi 
      214122, China.
FAU - Jiang, Yuge
AU  - Jiang Y
AD  - State Key Laboratory of Food Science & Technology, Jiangnan University, Wuxi 
      214122, China.
FAU - Luo, Tingyu
AU  - Luo T
AD  - State Key Laboratory of Food Science & Technology, Jiangnan University, Wuxi 
      214122, China.
FAU - Xu, Yuncong
AU  - Xu Y
AD  - State Key Laboratory of Food Science & Technology, Jiangnan University, Wuxi 
      214122, China.
FAU - Le, Guowei
AU  - Le G
AD  - State Key Laboratory of Food Science & Technology, Jiangnan University, Wuxi 
      214122, China State Key Laboratory of Food Science and Technology, Food Nutrition 
      and Functional Food Engineering Technology Research Center, Wuxi 214122, China.
FAU - Shi, Yonghui
AU  - Shi Y
AD  - State Key Laboratory of Food Science & Technology, Jiangnan University, Wuxi 
      214122, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Wei Sheng Yan Jiu
JT  - Wei sheng yan jiu = Journal of hygiene research
JID - 9426367
RN  - 0 (Blood Glucose)
RN  - 0 (GABA Agents)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - 5W494URQ81 (Streptozocin)
RN  - EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta)
SB  - IM
MH  - Animals
MH  - Blood Glucose/analysis
MH  - Diabetes Mellitus, Experimental/*drug therapy
MH  - Diabetes Mellitus, Type 2/*drug therapy
MH  - GABA Agents/*pharmacology
MH  - Glycogen Synthase Kinase 3 beta
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - *Oryza
MH  - Oxidative Stress/*drug effects
MH  - Streptozocin/*adverse effects
MH  - gamma-Aminobutyric Acid/*pharmacology
OTO - NOTNLM
OT  - germinated brown rice
OT  - oxidative stress
OT  - pancreas protection
OT  - type 2 diabetes
OT  - gamma-aminobutyric acid
EDAT- 2019/05/28 06:00
MHDA- 2019/08/30 06:00
CRDT- 2019/05/29 06:00
PHST- 2019/05/29 06:00 [entrez]
PHST- 2019/05/28 06:00 [pubmed]
PHST- 2019/08/30 06:00 [medline]
PST - ppublish
SO  - Wei Sheng Yan Jiu. 2019 Mar;48(2):179-199.