PMID- 31133311
OWN - NLM
STAT- MEDLINE
DCOM- 20200701
LR  - 20220531
IS  - 0737-0806 (Print)
IS  - 0737-0806 (Linking)
VI  - 77
DP  - 2019 Jun
TI  - Effect of Mare Colostrum in Extenders for Freezing Stallion Semen.
PG  - 23-27
LID - S0737-0806(18)30774-3 [pii]
LID - 10.1016/j.jevs.2019.02.010 [doi]
AB  - This study aimed to evaluate the addition of mare colostrum in stallion freezing 
      extenders to improve sperm quality. First, colostrum samples were collected from 
      four mares after the foal's birth and their composition was determined. 
      Ejaculates were collected from nine fertile stallions. Sperm samples were pooled, 
      diluted, and cryopreserved into three experimental extender groups: Lactose-based 
      extender supplemented with mare colostrum (20%), lactose-based extender 
      supplemented with egg yolk (20%), and BotuCrio. The quality of the post-thaw 
      semen samples were evaluated assessing sperm motility by means of 
      computer-assisted analysis, viability by SYBR-14 and propidium iodine (PI) stain, 
      acrosome integrity by fluorescein isothiocyanate and peanut agglutinine 
      (FITC-PNA) and PI stain, plasma membrane functionality by hypo-osmotic swelling 
      (HOS) test, and DNA denaturation by acridine orange (AO) test. There were no 
      significant differences in the percentages of total motility, acrosome integrity, 
      and DNA fragmentation among the extenders after thawing. Kinematics parameters 
      showed significantly higher values in BotuCrio than in lactose extenders (P < 
      .05). BotuCrio and lactose colostrum extender yielded significantly better rates 
      for HOS-test, linearity, straightness, and wobble than egg-yolk extender (P < 
      .05). However, in relation to sperm viability, lactose egg yolk extender showed 
      significantly better results in comparison to the others seminal experimental 
      media (P < .05). In conclusion, the incorporation of mare colostrum into 
      cryopreservation media protected the sperm against cold-shock; therefore, it may 
      be a good cryoprotectant agent alternative in extenders for freezing stallion 
      semen.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Alvarez, Cristina
AU  - Alvarez C
AD  - Chief Veterinary Officer of the Veterinary Unit, Zaragoza, Spain. Electronic 
      address: calvsan@gmail.com.
FAU - Luno, Victoria
AU  - Luno V
AD  - Department of Animal Pathology, Faculty of Veterinary Medicine, Instituto 
      Agroalimentario de Aragon IA2 (Universidad de Zaragoza-CITA), Zaragoza, Spain.
FAU - Gonzalez, Noelia
AU  - Gonzalez N
AD  - Department of Animal Pathology, Faculty of Veterinary Medicine, Instituto 
      Agroalimentario de Aragon IA2 (Universidad de Zaragoza-CITA), Zaragoza, Spain.
FAU - Guerra, Pilar
AU  - Guerra P
AD  - Chief Veterinary Officer of the Veterinary Unit, Jerez de la Frontera, Cadiz, 
      Spain.
FAU - Gil, Lydia
AU  - Gil L
AD  - Department of Animal Pathology, Faculty of Veterinary Medicine, Instituto 
      Agroalimentario de Aragon IA2 (Universidad de Zaragoza-CITA), Zaragoza, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190221
PL  - United States
TA  - J Equine Vet Sci
JT  - Journal of equine veterinary science
JID - 8216840
SB  - IM
MH  - Animals
MH  - Colostrum/*physiology
MH  - Female
MH  - Freezing
MH  - Horses
MH  - Male
MH  - Pregnancy
MH  - *Semen
MH  - Semen Preservation/methods/*veterinary
MH  - Sperm Motility
OTO - NOTNLM
OT  - Cryopreservation
OT  - Mare colostrum
OT  - Semen
OT  - Stallion
EDAT- 2019/05/28 06:00
MHDA- 2020/07/02 06:00
CRDT- 2019/05/29 06:00
PHST- 2018/12/07 00:00 [received]
PHST- 2019/01/22 00:00 [revised]
PHST- 2019/02/13 00:00 [accepted]
PHST- 2019/05/29 06:00 [entrez]
PHST- 2019/05/28 06:00 [pubmed]
PHST- 2020/07/02 06:00 [medline]
AID - S0737-0806(18)30774-3 [pii]
AID - 10.1016/j.jevs.2019.02.010 [doi]
PST - ppublish
SO  - J Equine Vet Sci. 2019 Jun;77:23-27. doi: 10.1016/j.jevs.2019.02.010. Epub 2019 
      Feb 21.