PMID- 31134714
OWN - NLM
STAT- MEDLINE
DCOM- 20200618
LR  - 20200618
IS  - 1098-2795 (Electronic)
IS  - 1040-452X (Linking)
VI  - 86
IP  - 8
DP  - 2019 Aug
TI  - Functional importance of palmitoyl protein thioesterase 1 (PPT1) expression by 
      Sertoli cells in mediating cholesterol metabolism and maintenance of sperm 
      quality.
PG  - 984-998
LID - 10.1002/mrd.23173 [doi]
AB  - Sertoli cells are a type of nurse cell in the seminiferous epithelium that are 
      crucial for sustaining spermatogenesis by extending nutritional and energy 
      support to the developing germ cells. Dysfunction of Sertoli cells could cause 
      disordered spermatogenesis and reduced fertility in males. In this study, we 
      focused on the expression and function of palmitoyl protein thioesterase 1 
      (PPT1), a lysosomal depalmitoylating enzyme, in Sertoli cells. Here, we show that 
      PPT1 expression in Sertoli cells is responsive to cholesterol treatment and that 
      specific knockout of Ppt1 in Sertoli cells causes male subfertility associated 
      with poor sperm quality and a high ratio of sperm deformity. Specifically, Ppt1 
      deficiency leads to poor cell variably accompanied with abnormal lysosome 
      accumulation and increased cholesterol levels in Sertoli cells. Further, Ppt1 
      deficiency results in poor adhesion of developing germ cells to Sertoli cells in 
      the seminiferous epithelium, which is likely to be responsible for the reduced 
      male fertility as a consequence of declines in sperm count and motility as well 
      as a high incidence of sperm head deformity. In summary, PPT1 affects sperm 
      quality and male fertility through regulating lysosomal function and cholesterol 
      metabolism in Sertoli cells.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Zhao, Wenzhen
AU  - Zhao W
AD  - Department of Histology and Embryology, School of Basic Medical Science, Dali 
      University, Yunnan, China.
AD  - Institute of Reproductive Medicine, Dali University, Yunnan, China.
FAU - Su, Juan
AU  - Su J
AD  - Department of Physiology and Pathophysiology, School of Basic Medical Science, 
      Dali University, Yunnan, China.
FAU - Wang, Yuntao
AU  - Wang Y
AD  - Department of Histology and Embryology, School of Basic Medical Science, Dali 
      University, Yunnan, China.
FAU - Qian, Tijun
AU  - Qian T
AD  - Vector Laboratory, Institute of Pathogens and Vectors, Dali University, Yunnan, 
      China.
FAU - Liu, Yue
AU  - Liu Y
AUID- ORCID: 0000-0002-2700-922X
AD  - Department of Histology, Embryology, Genetics and Developmental Biology, Shanghai 
      Key Laboratory for Reproductive Medicine, Shanghai Jiao Tong University School of 
      Medicine, Shanghai, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190527
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 3.1.2.- (Thiolester Hydrolases)
RN  - EC 3.1.2.22 (palmitoyl-protein thioesterase)
SB  - IM
MH  - Animals
MH  - Cholesterol/*metabolism
MH  - *Fertility
MH  - *Gene Expression Regulation, Enzymologic
MH  - Male
MH  - Mice
MH  - Seminiferous Tubules/cytology/enzymology
MH  - Sertoli Cells/cytology/*enzymology
MH  - Sperm Count
MH  - Spermatozoa/cytology/*enzymology
MH  - Thiolester Hydrolases/*biosynthesis
OTO - NOTNLM
OT  - PPT1
OT  - Sertoli cell
OT  - cholesterol metabolism
OT  - lysosome
OT  - sperm quality
EDAT- 2019/05/28 06:00
MHDA- 2020/06/19 06:00
CRDT- 2019/05/29 06:00
PHST- 2018/12/10 00:00 [received]
PHST- 2019/04/23 00:00 [revised]
PHST- 2019/05/01 00:00 [accepted]
PHST- 2019/05/28 06:00 [pubmed]
PHST- 2020/06/19 06:00 [medline]
PHST- 2019/05/29 06:00 [entrez]
AID - 10.1002/mrd.23173 [doi]
PST - ppublish
SO  - Mol Reprod Dev. 2019 Aug;86(8):984-998. doi: 10.1002/mrd.23173. Epub 2019 May 27.