PMID- 31137385
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230308
IS  - 1944-9097 (Electronic)
IS  - 0362-028X (Linking)
VI  - 58
IP  - 8
DP  - 1995 Aug
TI  - Evaluation of Coagulase Activity and Protein A Production for the Identification 
      of Staphylococcus aureus.
PG  - 858-862
LID - 10.4315/0362-028X-58.8.858 [doi]
AB  - The activities of coagulase and thermostable nuclease (TNase) and the production 
      of protein A were studied in 338 bacterial strains. These included 213 isolates 
      of Staphylococcus aureus to determine which characteristic was most specific for 
      the identification of S. aureus . The evaluation of different protocols for 
      interpretation of coagulase results was also undertaken. Protein A was analyzed 
      by a sandwich enzyme-linked immunosorbent assay using microtiter plates coated 
      with antiprotein A antibodies. Coagulase activities were determined according to 
      the criteria recommended by Association of Official Analytical Chemists (AOAC; 
      any degree of clot formation is a positive reaction), American Public Health 
      Association (APHA; coagulase activities >/= 3+ are positive reactions), and the 
      Bacteriological Analytical Manual (BAM; only 4+ reaction is positive). It was 
      found that the AOAC protocol, which had a test sensitivity of 97.7% and a 
      specificity of 95.1% and could be completed within six hours, was more practical 
      than the methods used by APHA and BAM. Compared with coagu1ase and TNase, protein 
      A was a better marker of S. aureus ; a high sensitivity (100%) and specificity 
      (96.8%) were obtained by using protein A for the identification of S. aureus .
FAU - Chang, Tsung C
AU  - Chang TC
AD  - Food Industry Research and Development Institute, P.O. Box 246, Hsinchu 300, 
      Taiwan, Republic of China.
FAU - Huang, Su H
AU  - Huang SH
AD  - Food Industry Research and Development Institute, P.O. Box 246, Hsinchu 300, 
      Taiwan, Republic of China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Food Prot
JT  - Journal of food protection
JID - 7703944
OTO - NOTNLM
OT  - Coagulase
OT  - protein A
OT  - thermostable nuclease
EDAT- 1995/08/01 00:00
MHDA- 1995/08/01 00:01
CRDT- 2019/05/30 06:00
PHST- 2019/05/30 06:00 [entrez]
PHST- 1995/08/01 00:00 [pubmed]
PHST- 1995/08/01 00:01 [medline]
AID - S0362-028X(22)05179-1 [pii]
AID - 10.4315/0362-028X-58.8.858 [doi]
PST - ppublish
SO  - J Food Prot. 1995 Aug;58(8):858-862. doi: 10.4315/0362-028X-58.8.858.