PMID- 31137628
OWN - NLM
STAT- MEDLINE
DCOM- 20200727
LR  - 20200727
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 11
IP  - 5
DP  - 2019 May 24
TI  - Detection Patterns of Porcine Parvovirus (PPV) and Novel Porcine Parvoviruses 2 
      through 6 (PPV2-PPV6) in Polish Swine Farms.
LID - 10.3390/v11050474 [doi]
LID - 474
AB  - Porcine parvovirus (PPV) is a major causative agent in reproductive failure, but 
      in the last two decades many novel porcine parvoviruses were described and 
      designated as porcine parvovirus 2 through 6 (PPV2-PPV6). However, their role for 
      pig health is largely unknown. The aim of this study was to better understand the 
      on-farm prevalence of PPVs in different age groups of pigs, and to assess the 
      diagnostic applicability of testing different diagnostic materials. In total, 271 
      oral fluids, 1244 serum samples, and 1238 fecal samples were collected from 
      3-21-week-old pigs from 19 farms, and after pooling by 4-6, tested by real-time 
      PCR. The results showed that PPVs are widely spread in Poland and that the 
      highest detection rates were obtained for oral fluids (ranging from 10.7% (PPV1) 
      to 48.7% (PPV2)). Fattening pigs were the age group with the most frequent 
      detection of PPVs (ranging from 8.6% (PPV1) to 49.1% (PPV2)). Porcine 
      parvoviruses were detected mostly in growing-finishing pigs and the infection 
      persisted until the late fattening period, which may suggest the chronic 
      character of the infection (especially for PPV2, which was found to commonly 
      infect animals of all ages). Particularly low Ct values detected for PPV2, PPV3, 
      PPV5, and PPV6 in serum pools from some farms suggested that these viruses may 
      cause high levels of viremia in one or more individuals included in these pools. 
      Further studies are needed to quantify the levels of PPVs viremia and to assess 
      the impact in co-infections with other, often endemic pig viruses, such as 
      porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory 
      syndrome virus (PRRSV).
FAU - Milek, Dagmara
AU  - Milek D
AD  - Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary 
      Medicine, Warsaw University of Life Sciences, Nowoursynowska 159C, 02-776 Warsaw, 
      Poland. dagmara_milek@sggw.pl.
FAU - Wozniak, Aleksandra
AU  - Wozniak A
AD  - Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary 
      Medicine, Warsaw University of Life Sciences, Nowoursynowska 159C, 02-776 Warsaw, 
      Poland. aleksandra_wozniak@sggw.pl.
FAU - Guzowska, Magdalena
AU  - Guzowska M
AD  - Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw 
      University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland. 
      magdalena_guzowska@sggw.pl.
FAU - Stadejek, Tomasz
AU  - Stadejek T
AUID- ORCID: 0000-0002-8889-8120
AD  - Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary 
      Medicine, Warsaw University of Life Sciences, Nowoursynowska 159C, 02-776 Warsaw, 
      Poland. tomasz_stadejek@sggw.pl.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190524
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - DNA, Viral
MH  - *Farms
MH  - Genotype
MH  - Parvoviridae Infections/*veterinary
MH  - Parvovirus, Porcine/*classification/*genetics
MH  - Poland/epidemiology
MH  - Public Health Surveillance
MH  - Real-Time Polymerase Chain Reaction
MH  - Swine
MH  - Swine Diseases/diagnosis/epidemiology/*virology
PMC - PMC6563502
OTO - NOTNLM
OT  - fatteners
OT  - feces
OT  - oral fluid
OT  - pig
OT  - piglets
OT  - porcine parvoviruses
OT  - real-time PCR
OT  - serum
OT  - weaners
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2019/05/30 06:00
MHDA- 2020/07/28 06:00
PMCR- 2019/05/01
CRDT- 2019/05/30 06:00
PHST- 2019/04/14 00:00 [received]
PHST- 2019/05/17 00:00 [revised]
PHST- 2019/05/22 00:00 [accepted]
PHST- 2019/05/30 06:00 [entrez]
PHST- 2019/05/30 06:00 [pubmed]
PHST- 2020/07/28 06:00 [medline]
PHST- 2019/05/01 00:00 [pmc-release]
AID - v11050474 [pii]
AID - viruses-11-00474 [pii]
AID - 10.3390/v11050474 [doi]
PST - epublish
SO  - Viruses. 2019 May 24;11(5):474. doi: 10.3390/v11050474.