PMID- 31138255
OWN - NLM
STAT- MEDLINE
DCOM- 20191227
LR  - 20200225
IS  - 1746-1596 (Electronic)
IS  - 1746-1596 (Linking)
VI  - 14
IP  - 1
DP  - 2019 May 28
TI  - Serum expression of selected miRNAs in patients with laryngeal squamous cell 
      carcinoma (LSCC).
PG  - 49
LID - 10.1186/s13000-019-0823-3 [doi]
LID - 49
AB  - BACKGROUND: The aim of the present study was to identify specific serum miRNAs 
      (preoperative serum samples compared to healthy controls) as potential diagnostic 
      markers for detection in laryngeal squamous cell carcinoma (LSCC). Serum samples 
      obtained from 66 patients with LSCC were compared with 100 healthy control 
      subjects. Additionally, miRNA levels were evaluated to identify possible 
      correlations with clinicopathological features. METHODS: The expression of 377 
      miRNAs (screening set) was evaluated by microarray screening. The most 
      differentially expressed miRNAs were validated by high-throughput real-time 
      quantitative polymerase chain reaction (RT-qPCR) in the group of LSCC patients 
      and healthy controls. Receiver-operator characteristic (ROC) curve analysis was 
      conducted to evaluate the diagnostic accuracy of the highly and significantly 
      identified deregulated miRNA(s) as potential candidate biomarker(s). RESULTS: 
      According to the array analysis, eleven miRNAs revealed an altered expression 
      profile. The levels of serum expression of miR-31, miR-141, miR-149a, miR-182, 
      LET-7a, miR-4853p, miR-122 and miR-33 were up-regulated, and those of miR-145, 
      miR-223 and miR-133a down-regulated, in the LSCC group compared to healthy 
      controls. ROC curve analyses revealed an AUC (area under the ROC curve) of 1.00 
      (95%Cl: 0.999-1.00; P <  0.001) for miR-31 and LET-7a, 1.00 (95%Cl: 1.00-1.00; 
      P <  0.001) for miR-33 respectively, indicating that these three miRNAs had an 
      additive effect regarding diagnostic value. No statistically significant 
      differences were found between the serum levels of these eleven miRNAs and the 
      tested clinicopathological features. CONCLUSION: Our findings outline a distinct 
      miRNA expression profile in laryngeal cancer (LC) cases which can be used to 
      diagnose LSCC patients with high sensitivity and specificity. Particular miRNA 
      signatures (miR-31, LET-7a and miR-33) may be considered as novel, non-invasive 
      biomarkers for LC diagnosis. TRIAL REGISTRATION: Registration number: 
      RNN/203/13/KE. Date of registration 18.06.2013r.
FAU - Lucas Grzelczyk, Weronika
AU  - Lucas Grzelczyk W
AUID- ORCID: 0000-0002-3897-6266
AD  - Department of Otolaryngology, Medical University of Lodz, Norbert Barlicki 
      Memorial Teaching Hospital, Lodz, Poland. weronika.lucas@gmail.com.
FAU - Szemraj, Janusz
AU  - Szemraj J
AD  - Department of Medical Biochemistry, Medical University of Lodz, Lodz, Poland.
FAU - Kwiatkowska, Sylwia
AU  - Kwiatkowska S
AD  - Department of Pneumonology, Norbert Barlicki Memorial Teaching Hospital, Lodz, 
      Poland.
FAU - Jozefowicz-Korczynska, Magdalena
AU  - Jozefowicz-Korczynska M
AD  - Department of Otolaryngology, Medical University of Lodz, Norbert Barlicki 
      Memorial Teaching Hospital, Lodz, Poland.
LA  - eng
GR  - 503/2-03602/503-21-002/Uniwersytet Medyczny w Lodzi/
PT  - Clinical Trial
PT  - Journal Article
DEP - 20190528
PL  - England
TA  - Diagn Pathol
JT  - Diagnostic pathology
JID - 101251558
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Squamous Cell/blood/*diagnosis/pathology
MH  - Down-Regulation
MH  - Female
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Neoplastic/*genetics
MH  - Humans
MH  - Laryngeal Neoplasms/blood/*diagnosis/pathology
MH  - Male
MH  - MicroRNAs/*blood
MH  - Middle Aged
MH  - ROC Curve
MH  - Up-Regulation
PMC - PMC6540364
OTO - NOTNLM
OT  - Circulating miRNAs
OT  - Laryngeal squamous cell carcinoma
OT  - Serum
COIS- The authors declare that they have no competing interests.
EDAT- 2019/05/30 06:00
MHDA- 2019/12/28 06:00
PMCR- 2019/05/28
CRDT- 2019/05/30 06:00
PHST- 2018/12/06 00:00 [received]
PHST- 2019/05/10 00:00 [accepted]
PHST- 2019/05/30 06:00 [entrez]
PHST- 2019/05/30 06:00 [pubmed]
PHST- 2019/12/28 06:00 [medline]
PHST- 2019/05/28 00:00 [pmc-release]
AID - 10.1186/s13000-019-0823-3 [pii]
AID - 823 [pii]
AID - 10.1186/s13000-019-0823-3 [doi]
PST - epublish
SO  - Diagn Pathol. 2019 May 28;14(1):49. doi: 10.1186/s13000-019-0823-3.