PMID- 31138404
OWN - NLM
STAT- MEDLINE
DCOM- 20200302
LR  - 20210920
IS  - 1879-3592 (Electronic)
IS  - 1383-5718 (Linking)
VI  - 841
DP  - 2019 May
TI  - Assessment of genotoxicity and cholinesterase activity among women workers 
      occupationally exposed to pesticides in tea garden.
PG  - 1-7
LID - S1383-5718(18)30172-4 [pii]
LID - 10.1016/j.mrgentox.2019.03.002 [doi]
AB  - To assess the effects of exposure to pesticides in tea garden women workers, the 
      present study evaluated the activities of erythrocyte acetylcholinesterase (AChE) 
      and plasma butyrylcholinesterase (BChE) as biomarkers of pesticide exposure and 
      DNA damage by comet assay as a biomarker of genotoxicity in peripheral blood 
      lymphocytes. We examined 143 women subjects including 77 women workers exposed to 
      pesticide in tea garden (exposed) and 66 women subject with no history of 
      occupational exposure to pesticides (non-exposed). The two groups had similar 
      mean ages, BMI and personal habits. The results revealed that exposed group had 
      lower activity of AChE (2.86 +/- 0.75 vs. 3.93 +/- 0.87 U/ml) BChE (2.02 +/- 0.74 vs. 
      2.60 +/- 0.74 U/ml, p < 0.001) than those of non-exposed group. The mean comet 
      parameters such as tail length (TL), % DNA in tail, tail moment (TM) and olive 
      tail moment (OTM) used to measure DNA damage were significantly elevated in 
      exposed workers compared to non-exposed (p < 0.001). There was no variation 
      observed in Cholinesterase (ChEs) activity and Comet assay parameter based on 
      age, personal habits, years of exposure and use of personal protective equipments 
      (PPEs) except BChE activity. The multiple regression analysis showed no 
      association of confounding factors on end points measured except slight negative 
      association of BMI with tail length. Therefore this study suggests that the 
      workers in tea garden might have exposed to pesticide through their occupation on 
      a regular interval. Although this study could able to associate it with their 
      occupational exposure, the role of other DNA damaging factors cannot be ruled 
      out. Our study finding indicate the necessity of periodic monitoring of these 
      biomarkers along with imparting education and training to minimise the risk.
CI  - Copyright (c) 2019. Published by Elsevier B.V.
FAU - V, Dhananjayan
AU  - V D
AD  - Regional Occupational Health Centre (Southern), ICMR Complex, Devanahalli TK, 
      Bangalore, 562 110, India. Electronic address: dhananjayan.v@gov.in.
FAU - B, Ravichandran
AU  - B R
AD  - Regional Occupational Health Centre (Southern), ICMR Complex, Devanahalli TK, 
      Bangalore, 562 110, India.
FAU - K, Panjakumar
AU  - K P
AD  - Regional Occupational Health Centre (Southern), ICMR Complex, Devanahalli TK, 
      Bangalore, 562 110, India.
FAU - K, Kalaiselvi
AU  - K K
AD  - Department of Environmental Science, PSGCAS, Coimbatore, Tamil Nadu, 641 014, 
      India.
FAU - Rajasekar, Kausic
AU  - Rajasekar K
AD  - Primary Health Centre, Valparai, Tamil Nadu, 642 127, India.
FAU - A, Mala
AU  - A M
AD  - Regional Occupational Health Centre (Southern), ICMR Complex, Devanahalli TK, 
      Bangalore, 562 110, India.
FAU - G, Avinash
AU  - G A
AD  - Regional Occupational Health Centre (Southern), ICMR Complex, Devanahalli TK, 
      Bangalore, 562 110, India.
FAU - K, Shridhar
AU  - K S
AD  - Regional Occupational Health Centre (Southern), ICMR Complex, Devanahalli TK, 
      Bangalore, 562 110, India.
FAU - A, Manju
AU  - A M
AD  - Department of Environmental Science, PSGCAS, Coimbatore, Tamil Nadu, 641 014, 
      India.
FAU - Wilson, Rajesh
AU  - Wilson R
AD  - Primary Health Centre, Valparai, Tamil Nadu, 642 127, India.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190305
PL  - Netherlands
TA  - Mutat Res Genet Toxicol Environ Mutagen
JT  - Mutation research. Genetic toxicology and environmental mutagenesis
JID - 101632149
RN  - 0 (Biomarkers)
RN  - 0 (Pesticides)
RN  - 0 (Tea)
RN  - EC 3.1.1.8 (Cholinesterases)
SB  - IM
MH  - Adult
MH  - Biomarkers/metabolism
MH  - Case-Control Studies
MH  - Cholinesterases/*metabolism
MH  - Comet Assay
MH  - DNA Damage/drug effects
MH  - Female
MH  - Gardens
MH  - Humans
MH  - Lymphocytes/drug effects
MH  - Middle Aged
MH  - Mutagenicity Tests
MH  - Occupational Exposure/*adverse effects
MH  - Pesticides/*toxicity
MH  - Tea
OTO - NOTNLM
OT  - Cholinesterase activity
OT  - Comet assay
OT  - DNA damage
OT  - Occupational pesticide exposure
OT  - Women workers
EDAT- 2019/05/30 06:00
MHDA- 2020/03/03 06:00
CRDT- 2019/05/30 06:00
PHST- 2018/05/23 00:00 [received]
PHST- 2019/02/26 00:00 [revised]
PHST- 2019/03/04 00:00 [accepted]
PHST- 2019/05/30 06:00 [entrez]
PHST- 2019/05/30 06:00 [pubmed]
PHST- 2020/03/03 06:00 [medline]
AID - S1383-5718(18)30172-4 [pii]
AID - 10.1016/j.mrgentox.2019.03.002 [doi]
PST - ppublish
SO  - Mutat Res Genet Toxicol Environ Mutagen. 2019 May;841:1-7. doi: 
      10.1016/j.mrgentox.2019.03.002. Epub 2019 Mar 5.