PMID- 31139241
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231011
IS  - 1746-4811 (Print)
IS  - 1746-4811 (Electronic)
IS  - 1746-4811 (Linking)
VI  - 15
DP  - 2019
TI  - A high-throughput delayed fluorescence method reveals underlying differences in 
      the control of circadian rhythms in Triticum aestivum and Brassica napus.
PG  - 51
LID - 10.1186/s13007-019-0436-6 [doi]
LID - 51
AB  - BACKGROUND: A robust circadian clock has been implicated in plant resilience, 
      resource-use efficiency, competitive growth and yield. A huge number of 
      physiological processes are under circadian control in plants including: 
      responses to biotic and abiotic stresses; flowering time; plant metabolism; and 
      mineral uptake. Understanding how the clock functions in crops such as Triticum 
      aestivum (bread wheat) and Brassica napus (oilseed rape) therefore has great 
      agricultural potential. Delayed fluorescence (DF) imaging has been shown to be 
      applicable to a wide range of plant species and requires no genetic 
      transformation. Although DF has been used to measure period length of both 
      mutants and wild ecotypes of Arabidopsis, this assay has never been 
      systematically optimised for crop plants. The physical size of both B. napus and 
      T. aestivum led us to develop a representative sampling strategy which enables 
      high-throughput imaging of these crops. RESULTS: In this study, we describe the 
      plant-specific optimisation of DF imaging to obtain reliable circadian phenotypes 
      with the robustness and reproducibility to detect diverging periods between 
      cultivars of the same species. We find that the age of plant material, light 
      regime and temperature conditions all significantly effect DF rhythms and 
      describe the optimal conditions for measuring robust rhythms in each species. We 
      also show that sections of leaf can be used to obtain period estimates with 
      improved throughput for larger sample size experiments. CONCLUSIONS: We present 
      an optimized protocol for high-throughput phenotyping of circadian period 
      specific to two economically valuable crop plants. Application of this method 
      revealed significant differences between the periods of several widely grown 
      elite cultivars. This method also identified intriguing differential responses of 
      circadian rhythms in T. aestivum compared to B. napus; specifically the dramatic 
      change to rhythm robustness when plants were imaged under constant light versus 
      constant darkness. This points towards diverging networks underlying circadian 
      control in these two species.
FAU - Rees, Hannah
AU  - Rees H
AD  - 1Earlham Institute, Norwich Research Park, Norwich, NR4 7UG UK. ISNI: 0000 0004 
      0447 4123. GRID: grid.421605.4
AD  - 2Institute of Integrative Biology, University of Liverpool, Crown Street, 
      Liverpool, L69 7ZB UK. ISNI: 0000 0004 1936 8470. GRID: grid.10025.36
FAU - Duncan, Susan
AU  - Duncan S
AD  - 1Earlham Institute, Norwich Research Park, Norwich, NR4 7UG UK. ISNI: 0000 0004 
      0447 4123. GRID: grid.421605.4
FAU - Gould, Peter
AU  - Gould P
AD  - 2Institute of Integrative Biology, University of Liverpool, Crown Street, 
      Liverpool, L69 7ZB UK. ISNI: 0000 0004 1936 8470. GRID: grid.10025.36
FAU - Wells, Rachel
AU  - Wells R
AD  - 3John Innes Centre, Norwich Research Park, Norwich, NR4 7UH UK. ISNI: 0000 0001 
      2175 7246. GRID: grid.14830.3e
FAU - Greenwood, Mark
AU  - Greenwood M
AD  - 4Sainsbury Laboratory, University of Cambridge, Cambridge, CB2 1LR UK. ISNI: 
      0000000121885934. GRID: grid.5335.0
AD  - 5Department of Biochemistry, University of Cambridge, Cambridge, CB2 1QW UK. 
      ISNI: 0000000121885934. GRID: grid.5335.0
FAU - Brabbs, Thomas
AU  - Brabbs T
AD  - 1Earlham Institute, Norwich Research Park, Norwich, NR4 7UG UK. ISNI: 0000 0004 
      0447 4123. GRID: grid.421605.4
FAU - Hall, Anthony
AU  - Hall A
AUID- ORCID: 0000-0002-1806-020X
AD  - 1Earlham Institute, Norwich Research Park, Norwich, NR4 7UG UK. ISNI: 0000 0004 
      0447 4123. GRID: grid.421605.4
LA  - eng
GR  - BBS/E/J/000PR9788/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
PT  - Journal Article
DEP - 20190521
PL  - England
TA  - Plant Methods
JT  - Plant methods
JID - 101245798
PMC - PMC6530173
OTO - NOTNLM
OT  - Aging
OT  - Circadian period
OT  - D:D
OT  - Delayed fluorescence
OT  - Free-running conditions
OT  - Hexaploid wheat
OT  - L:L
OT  - Oilseed rape
OT  - Rhythm robustness
OT  - Temperature
COIS- Competing interestsThe authors declare that they have no competing interests.
EDAT- 2019/05/30 06:00
MHDA- 2019/05/30 06:01
PMCR- 2019/05/21
CRDT- 2019/05/30 06:00
PHST- 2018/12/04 00:00 [received]
PHST- 2019/05/10 00:00 [accepted]
PHST- 2019/05/30 06:00 [entrez]
PHST- 2019/05/30 06:00 [pubmed]
PHST- 2019/05/30 06:01 [medline]
PHST- 2019/05/21 00:00 [pmc-release]
AID - 436 [pii]
AID - 10.1186/s13007-019-0436-6 [doi]
PST - epublish
SO  - Plant Methods. 2019 May 21;15:51. doi: 10.1186/s13007-019-0436-6. eCollection 
      2019.