PMID- 3113979
OWN - NLM
STAT- MEDLINE
DCOM- 19871009
LR  - 20211203
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 17
IP  - 8
DP  - 1987 Aug
TI  - Comparison of the clonal diversity of the B cell repertoires in adult mice that 
      differ in the expression of cell surface IgD.
PG  - 1137-43
AB  - To determine whether the expression of surface IgD (sIgD) influences the extent 
      of the expressed B cell repertoire, the clonal diversity of the B cell population 
      in mice treated chronically with anti-IgD (delta) antibodies has been compared 
      with the B cell repertoire observed in control animals, using the splenic focus 
      limiting dilution B cell assay. The results show that the phosphorylcholine 
      (PC)-specific B cell precursor frequency in anti-delta antibody-treated mice is 
      increased when compared with that of control mice. Isotype and idiotype (T15) 
      analyses of PC clonal products from anti-delta antibody-treated and control mice 
      revealed no distributional differences. Analyses of the 2,4-dinitrophenyl (DNP)- 
      and fluoresein isothiocyanate-specific B cell repertoires confirmed that the 
      equal or increased precursor frequencies observed in anti-delta antibody-treated 
      mice are not specific for the PC antigen. The increased precursor frequency of B 
      cells from anti-delta antibody-treated mice was not the result of increased 
      homing of B cells from anti-delta antibody-treated mice to recipient spleens, 
      since B cells from control mice homed twice as well to recipient spleens as did B 
      cells from anti-delta antibody-treated mice. Other studies demonstrated that (a) 
      on average, antibody-secreting clones were generated more slowly when B cells 
      from anti-delta antibody-treated mice were used as a source of precursors than B 
      cells of control mice and (b) both sIg- spleen cells and sIg+ spleen cells from 
      anti-delta antibody-treated mice generated a higher frequency of specific 
      antibody-secreting clones than did the corresponding populations from control 
      mice. These observations suggest that a population of sIgM+sIgD- B cells exists 
      that resembles sIgD+ B cells rather than neonatal or xid B cells in its ability 
      to generate responses to PC and suggests that the sIgM+sIgD- B cells from 
      anti-delta antibody-treated mice are more responsive than are sIgM+IgD+ B cells, 
      regardless of antigenic specificity, to the stimuli provided in the splenic focus 
      system. Finally, this study suggests that the expression of sIgD does not 
      influence the extent of the expressed B cell repertoire.
FAU - Fultz, M J
AU  - Fultz MJ
FAU - Finkelman, F D
AU  - Finkelman FD
FAU - Metcalf, E S
AU  - Metcalf ES
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Dinitrobenzenes)
RN  - 0 (Fluoresceins)
RN  - 0 (Immunoglobulin D)
RN  - 0 (Immunoglobulin Idiotypes)
RN  - 0 (Immunoglobulin Isotypes)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Receptors, Antigen, B-Cell)
RN  - 0 (Thiocyanates)
RN  - 107-73-3 (Phosphorylcholine)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Animals
MH  - Antibodies, Anti-Idiotypic/pharmacology
MH  - *Antibody Diversity
MH  - Antibody-Producing Cells/cytology/*immunology
MH  - B-Lymphocytes/cytology/*immunology
MH  - Dinitrobenzenes/immunology
MH  - Fluorescein-5-isothiocyanate
MH  - Fluoresceins/immunology
MH  - Immunoglobulin D/*immunology
MH  - Immunoglobulin Idiotypes/analysis
MH  - Immunoglobulin Isotypes/analysis
MH  - Immunoglobulin M/analysis
MH  - Immunosuppression Therapy
MH  - Mice
MH  - Phosphorylcholine/immunology
MH  - Receptors, Antigen, B-Cell/*physiology
MH  - Spleen/cytology
MH  - Thiocyanates/immunology
EDAT- 1987/08/01 00:00
MHDA- 1987/08/01 00:01
CRDT- 1987/08/01 00:00
PHST- 1987/08/01 00:00 [pubmed]
PHST- 1987/08/01 00:01 [medline]
PHST- 1987/08/01 00:00 [entrez]
AID - 10.1002/eji.1830170810 [doi]
PST - ppublish
SO  - Eur J Immunol. 1987 Aug;17(8):1137-43. doi: 10.1002/eji.1830170810.