PMID- 31141206
OWN - NLM
STAT- MEDLINE
DCOM- 20200109
LR  - 20200109
IS  - 1600-0714 (Electronic)
IS  - 0904-2512 (Linking)
VI  - 48
IP  - 8
DP  - 2019 Sep
TI  - Effects of chronic treatment with botulinum toxin type A in salivary glands of 
      rats: Histological and immunohistochemical analyses.
PG  - 728-734
LID - 10.1111/jop.12887 [doi]
AB  - BACKGROUND: The intraglandular application of botulinum neurotoxin type A 
      (BoNT-A) is used in patients with neuromotor disorders to control the escape of 
      saliva. The aim of this study was to analyze the impact of repeated treatment 
      with BoNT-A on the submandibular-sublingual complex of rats. METHODS: A total of 
      35 Wistar rats were divided into three groups: control group (C), in which 
      animals were not treated; group B, treated with 2.5 U intraglandular injections 
      of BoNT-A (Prosigne((R)) ) and group G, that received bovine gelatine (Prosigne 
      stabilizer). Three applications were performed in intervals of 35 days. Twelve 
      and 35 days after ending the treatment, submandibular-sublingual complex was 
      collected for histological analysis. Immunohistochemical reactions for calponin 
      and specific muscle actin were also performed, besides detection of apoptosis by 
      terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) 
      assay. RESULTS: A decrease in mucosal and serous acini diameter was observed, 
      with increased interstitial space after 12 days of treatment with BoNT-A, which 
      was observed in a lesser degree on the 35th day. At 12 days, immunohistochemical 
      analysis revealed a decrease in myoepithelial cells of serous acini in group B. 
      TUNEL methods evidenced apoptosis in animals from group B. CONCLUSIONS: BoNT-A 
      caused histological and cellular changes in submandibular-sublingual complex, 
      followed by a tendency toward reversal after 35 days. The reversal characteristic 
      of cellular changes in the submandibular-sublingual complex suggests that this 
      BoNT-A formulation may be safely used for sialorrhea treatment.
CI  - (c) 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Regueira, Luciana Silva
AU  - Regueira LS
AD  - Professor Fernando Figueira Integral Medicine Institute, Recife, Brazil.
FAU - Baratella-Evencio, Liriane
AU  - Baratella-Evencio L
AD  - Department of Histology and Embryology, Universidade Federal de Pernambuco, 
      Recife, Brazil.
FAU - de Oliveira, Jaciel Benedito
AU  - de Oliveira JB
AD  - Department of Anatomy, Universidade Federal de Pernambuco, Recife, Brazil.
FAU - da Silva, Priscilla Suelen Oliveira Carvalho
AU  - da Silva PSOC
AD  - Department of Histology and Embryology, Universidade Federal de Pernambuco, 
      Recife, Brazil.
FAU - Valenca, Gabriela Andrade Lima
AU  - Valenca GAL
AD  - Department of Histology and Embryology, Universidade Federal de Pernambuco, 
      Recife, Brazil.
FAU - Conceicao, Juliana Cristina Peixoto
AU  - Conceicao JCP
AD  - Department of Histology and Embryology, Universidade Federal de Pernambuco, 
      Recife, Brazil.
FAU - da Cruz Perez, Danyel Elias
AU  - da Cruz Perez DE
AUID- ORCID: 0000-0002-4591-4645
AD  - Department of Clinical and Preventive Dentistry, School of Dentistry, 
      Universidade Federal de Pernambuco, Recife, Brazil.
LA  - eng
GR  - 001/Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/
PT  - Journal Article
DEP - 20190612
PL  - Denmark
TA  - J Oral Pathol Med
JT  - Journal of oral pathology & medicine : official publication of the International 
      Association of Oral Pathologists and the American Academy of Oral Pathology
JID - 8911934
RN  - EC 3.4.24.69 (Botulinum Toxins, Type A)
RN  - EC 3.4.24.69 (prosigne)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Botulinum Toxins, Type A/*pharmacology
MH  - Cattle
MH  - Epithelial Cells/drug effects
MH  - Female
MH  - Immunohistochemistry
MH  - Rats
MH  - Rats, Wistar
MH  - Saliva
MH  - Salivary Glands/*drug effects/pathology
MH  - Sialorrhea/drug therapy
OTO - NOTNLM
OT  - botulinum toxin type A
OT  - rats
OT  - salivary glands
EDAT- 2019/05/30 06:00
MHDA- 2020/01/10 06:00
CRDT- 2019/05/30 06:00
PHST- 2018/09/27 00:00 [received]
PHST- 2019/04/27 00:00 [revised]
PHST- 2019/05/27 00:00 [accepted]
PHST- 2019/05/30 06:00 [pubmed]
PHST- 2020/01/10 06:00 [medline]
PHST- 2019/05/30 06:00 [entrez]
AID - 10.1111/jop.12887 [doi]
PST - ppublish
SO  - J Oral Pathol Med. 2019 Sep;48(8):728-734. doi: 10.1111/jop.12887. Epub 2019 Jun 
      12.