PMID- 31143783
OWN - NLM
STAT- MEDLINE
DCOM- 20191212
LR  - 20220408
IS  - 2314-7156 (Electronic)
IS  - 2314-8861 (Print)
IS  - 2314-7156 (Linking)
VI  - 2019
DP  - 2019
TI  - The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL/6 
      and CBA Mice.
PG  - 7029726
LID - 10.1155/2019/7029726 [doi]
LID - 7029726
AB  - INTRODUCTION: Dendritic cells (DCs) control immune responses by modulating T and 
      B cells towards effector or tolerogenic responses. In this study, we evaluated 
      the effects of different immunosuppressive molecules on the phenotypic and 
      functional characteristics of primary dendritic cells from C57BL/6 and CBA mice. 
      METHODS: DCs were derived from bone marrow cells in the presence of rmGM-CSF and 
      rmIL-4. DCs were then treated with different types of immunosuppressive molecules 
      (rmIL-10, rmTGF-beta, and BAY 11-7082) and cocultured with syngeneic splenocytes. 
      The amount of CD4+CD25hiFoxP3+ Tregs, IL-10 expression, and proliferation were 
      evaluated. RESULTS: Tolerogenic factors were found to have different effects on 
      DCs C57Bl/6 mice. In C57Bl/6 mice, BAY 11-7082 alone had no effect on the 
      expression of DC maturation molecules (CD80, CD86). Transforming growth factor 
      beta (TGF-beta), alone and in combination with BAY 11-7082, reduced the expression 
      of these molecules. Cocultivation of DCs with splenocytes in the presence of 
      TGF-beta and BAY 11-7082 favored regulatory T cell (CD4+CD25hiFoxP3+) 
      differentiation and disfavored differentiation of CD4+ T cells producing IL-10. 
      In CBA mice, we found that rmIL-10 and rmTGF-beta have a weak effect on maturation 
      of DCs and their functional properties to induce Treg cells and IL-10 production. 
      CONCLUSION: These results indicate that TGF-beta and IL-10 have different effects on 
      the phenotypic and functional characteristics of DCs and that the NF-kappaB 
      inhibitor, BAY 11-7082, has no synergistic effect on these treatments. In mice 
      with an opposite nature of the immune response, the effects of immunoregulatory 
      cytokines (IL-10 and TGF-b) differ on maturation of dendritic cells.
FAU - Kurilin, Vasiliy V
AU  - Kurilin VV
AUID- ORCID: 0000-0002-5617-0450
AD  - Laboratory of Molecular Immunology, Federal State Budgetary Institution "Research 
      Institute of Fundamental and Clinical Immunology", Yadrintsevskaya St. 14, 
      Novosibirsk, 630099, Russia.
FAU - Khantakova, Julia N
AU  - Khantakova JN
AUID- ORCID: 0000-0001-9281-9002
AD  - Laboratory of Molecular Immunology, Federal State Budgetary Institution "Research 
      Institute of Fundamental and Clinical Immunology", Yadrintsevskaya St. 14, 
      Novosibirsk, 630099, Russia.
AD  - Novosibirsk State University, Pirogova St. 2, Novosibirsk, 630090, Russia.
FAU - Tereschenko, Valeriy P
AU  - Tereschenko VP
AUID- ORCID: 0000-0002-1620-6447
AD  - Laboratory of Molecular Immunology, Federal State Budgetary Institution "Research 
      Institute of Fundamental and Clinical Immunology", Yadrintsevskaya St. 14, 
      Novosibirsk, 630099, Russia.
FAU - Lopatnikova, Julia A
AU  - Lopatnikova JA
AUID- ORCID: 0000-0003-0089-5054
AD  - Laboratory of Molecular Immunology, Federal State Budgetary Institution "Research 
      Institute of Fundamental and Clinical Immunology", Yadrintsevskaya St. 14, 
      Novosibirsk, 630099, Russia.
FAU - Obleukhova, Irina A
AU  - Obleukhova IA
AUID- ORCID: 0000-0003-2180-3243
AD  - Laboratory of Molecular Immunology, Federal State Budgetary Institution "Research 
      Institute of Fundamental and Clinical Immunology", Yadrintsevskaya St. 14, 
      Novosibirsk, 630099, Russia.
FAU - Sennikov, Sergey V
AU  - Sennikov SV
AUID- ORCID: 0000-0002-7366-7768
AD  - Laboratory of Molecular Immunology, Federal State Budgetary Institution "Research 
      Institute of Fundamental and Clinical Immunology", Yadrintsevskaya St. 14, 
      Novosibirsk, 630099, Russia.
AD  - Novosibirsk State University, Pirogova St. 2, Novosibirsk, 630090, Russia.
LA  - eng
PT  - Journal Article
DEP - 20190418
PL  - Egypt
TA  - J Immunol Res
JT  - Journal of immunology research
JID - 101627166
RN  - 0 (3-(4-methylphenylsulfonyl)-2-propenenitrile)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (NF-kappa B)
RN  - 0 (Nitriles)
RN  - 0 (Sulfones)
RN  - 0 (Transforming Growth Factor beta)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dendritic Cells/*immunology
MH  - Drug Synergism
MH  - Drug Therapy, Combination
MH  - Immunosuppressive Agents/*pharmacology
MH  - Interleukin-10/pharmacology
MH  - Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred CBA
MH  - NF-kappa B/antagonists & inhibitors
MH  - Nitriles/pharmacology
MH  - Primary Cell Culture
MH  - Sulfones/pharmacology
MH  - T-Lymphocytes, Regulatory/*immunology
MH  - Transforming Growth Factor beta/pharmacology
PMC - PMC6501430
EDAT- 2019/05/31 06:00
MHDA- 2019/12/18 06:00
PMCR- 2019/04/18
CRDT- 2019/05/31 06:00
PHST- 2018/10/03 00:00 [received]
PHST- 2019/01/28 00:00 [revised]
PHST- 2019/02/26 00:00 [accepted]
PHST- 2019/05/31 06:00 [entrez]
PHST- 2019/05/31 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/04/18 00:00 [pmc-release]
AID - 10.1155/2019/7029726 [doi]
PST - epublish
SO  - J Immunol Res. 2019 Apr 18;2019:7029726. doi: 10.1155/2019/7029726. eCollection 
      2019.