PMID- 31143910
OWN - NLM
STAT- MEDLINE
DCOM- 20191206
LR  - 20191217
IS  - 2042-650X (Electronic)
IS  - 2042-6496 (Linking)
VI  - 10
IP  - 6
DP  - 2019 Jun 19
TI  - Potential mechanisms mediating the protective effects of a peptide from walnut 
      (Juglans mandshurica Maxim.) against hydrogen peroxide induced neurotoxicity in 
      PC12 cells.
PG  - 3491-3501
LID - 10.1039/c8fo02557f [doi]
AB  - Amelioration of oxidative stress has been the main approach to improve 
      neurodegenerative disorders. In the present study, a walnut peptide with a strong 
      capacity of scavenging reactive oxygen species (ROS) was purified and identified 
      as EVSGPGLSPN by SEC, RP-HPLC, and HPLC-MS/MS. Treatment with EVSGPGLSPN could 
      significantly (P < 0.05) reduce ROS generation, and increase cell viability, and 
      superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-px) 
      activities in a dose-dependent manner in hydrogen peroxide induced PC12 cells. 
      Western blot and immunofluorescence analysis showed that EVSGPGLSPN suppressed 
      the expression of IKKbeta and p65 to inhibit NF-kappaB pathway activation, attenuating 
      the neurotoxic cascade by overexpression of IL-1beta and TNF-alpha. Moreover, EVSGPGLSPN 
      inhibited apoptosis by suppressing the expression of cytochrome C, caspase-9, 
      caspase-3, and PARP. Additionally, it also up-regulated the expression of p-CREB 
      and synaptophysin in oxidatively damaged PC12 cells. Thus, EVSGPGLSPN may protect 
      against hydrogen peroxide induced neurotoxicity by enhancing the activity of 
      antioxidant enzymes and blocking the NF-kappaB/caspase pathways.
FAU - Liu, Chunlei
AU  - Liu C
AD  - College of Food Science and Engineering, Jilin Agricultural University, Changchun 
      130118, P.R. China. minwh2000@jlau.edu.cn.
FAU - Guo, Yong
AU  - Guo Y
FAU - Zhao, Fanrui
AU  - Zhao F
FAU - Qin, Hanxiong
AU  - Qin H
FAU - Lu, Hongyan
AU  - Lu H
FAU - Fang, Li
AU  - Fang L
FAU - Wang, Ji
AU  - Wang J
FAU - Min, Weihong
AU  - Min W
LA  - eng
PT  - Journal Article
PL  - England
TA  - Food Funct
JT  - Food & function
JID - 101549033
RN  - 0 (Antioxidants)
RN  - 0 (NF-kappa B)
RN  - 0 (Peptides)
RN  - 0 (Plant Extracts)
RN  - 0 (Protective Agents)
RN  - 0 (Reactive Oxygen Species)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Animals
MH  - Antioxidants/metabolism
MH  - Apoptosis/drug effects
MH  - Caspases/genetics/metabolism
MH  - Catalase/genetics/metabolism
MH  - Hydrogen Peroxide/*toxicity
MH  - Juglans/*chemistry
MH  - NF-kappa B/genetics/metabolism
MH  - Neurons/cytology/*drug effects/metabolism
MH  - Oxidative Stress/drug effects
MH  - PC12 Cells
MH  - Peptides/*pharmacology
MH  - Plant Extracts/*pharmacology
MH  - Protective Agents/*pharmacology
MH  - Rats
MH  - Reactive Oxygen Species/metabolism
MH  - Superoxide Dismutase/genetics/metabolism
EDAT- 2019/05/31 06:00
MHDA- 2019/12/18 06:00
CRDT- 2019/05/31 06:00
PHST- 2019/05/31 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/05/31 06:00 [entrez]
AID - 10.1039/c8fo02557f [doi]
PST - ppublish
SO  - Food Funct. 2019 Jun 19;10(6):3491-3501. doi: 10.1039/c8fo02557f.