PMID- 31146160
OWN - NLM
STAT- MEDLINE
DCOM- 20191030
LR  - 20191030
IS  - 1873-6750 (Electronic)
IS  - 0160-4120 (Linking)
VI  - 129
DP  - 2019 Aug
TI  - Impacts of in vivo and in vitro exposures to tamoxifen: Comparative effects on 
      human cells and marine organisms.
PG  - 256-272
LID - S0160-4120(18)33231-8 [pii]
LID - 10.1016/j.envint.2019.05.014 [doi]
AB  - Tamoxifen (TAM) is a first generation-SERM administered for hormone 
      receptor-positive (HER+) breast cancer in both pre- and post-menopausal patients 
      and may undergo metabolic activation in organisms that share similar receptors 
      and thus face comparable mechanisms of response. The present study aimed to 
      assess whether environmental trace concentrations of TAM are bioavailable to the 
      filter feeder M. galloprovincialis (100 ng L(-1)) and to the deposit feeder N. 
      diversicolor (0.5, 10, 25 and 100 ng L(-1)) after 14 days of exposure. 
      Behavioural impairment (burrowing kinetic), neurotoxicity (AChE activity), 
      endocrine disruption by alkali-labile phosphate (ALP) content, oxidative stress 
      (SOD, CAT, GPXs activities), biotransformation (GST activity), oxidative damage 
      (LPO) and genotoxicity (DNA damage) were assessed. Moreover, this study also 
      pertained to compare TAM cytotoxicity effects to mussels and targeted human (i.e. 
      immortalized retinal pigment epithelium - RPE; and human transformed endothelial 
      cells - HeLa) cell lines, in a range of concentrations from 0.5 ng L(-1) to 
      50 mug L(-1). In polychaetes N. diversicolor, TAM exerted remarkable oxidative 
      stress and damage at the lowest concentration (0.5 ng L(-1)), whereas significant 
      genotoxicity was reported at the highest exposure level (100 ng L(-1)). In 
      mussels M. galloprovincialis, 100 ng L(-1) TAM caused endocrine disruption in 
      males, neurotoxicity, and an induction in GST activity and LPO byproducts in 
      gills, corroborating in genotoxicity over the exposure days. Although 
      cytotoxicity assays conducted with mussel haemocytes following in vivo exposure 
      was not effective, in vitro exposure showed to be a feasible alternative, with 
      comparable sensitivity to human cell line (HeLa).
CI  - Copyright (c) 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Fonseca, T G
AU  - Fonseca TG
AD  - CIMA, Centro de Investigacao Marinha e Ambiental, Universidade do Algarve, Campus 
      Gambelas, 8005-135 Faro, Portugal; NEPEA, Nucleo de Estudos em Poluicao e 
      Ecotoxicologia, Aquatica, Universidade Estadual Paulista (UNESP), Campus do 
      Litoral Paulista, Sao Vicente, SP 11330-900, Brazil.
FAU - Carrico, T
AU  - Carrico T
AD  - CIMA, Centro de Investigacao Marinha e Ambiental, Universidade do Algarve, Campus 
      Gambelas, 8005-135 Faro, Portugal.
FAU - Fernandes, E
AU  - Fernandes E
AD  - CIMA, Centro de Investigacao Marinha e Ambiental, Universidade do Algarve, Campus 
      Gambelas, 8005-135 Faro, Portugal.
FAU - Abessa, D M S
AU  - Abessa DMS
AD  - NEPEA, Nucleo de Estudos em Poluicao e Ecotoxicologia, Aquatica, Universidade 
      Estadual Paulista (UNESP), Campus do Litoral Paulista, Sao Vicente, SP 11330-900, 
      Brazil.
FAU - Tavares, A
AU  - Tavares A
AD  - Departamento de Ciencias Biomedicas e Medicina, Universidade do Algarve, Campus 
      Gambelas, 8005-135 Faro, Portugal.
FAU - Bebianno, M J
AU  - Bebianno MJ
AD  - CIMA, Centro de Investigacao Marinha e Ambiental, Universidade do Algarve, Campus 
      Gambelas, 8005-135 Faro, Portugal. Electronic address: mbebian@ualg.pt.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190527
PL  - Netherlands
TA  - Environ Int
JT  - Environment international
JID - 7807270
RN  - 094ZI81Y45 (Tamoxifen)
SB  - IM
MH  - Animals
MH  - Biotransformation
MH  - Bivalvia
MH  - DNA Damage
MH  - Female
MH  - Gills
MH  - Humans
MH  - Male
MH  - Oxidative Stress/drug effects
MH  - Tamoxifen/*pharmacology
OTO - NOTNLM
OT  - Biomarkers
OT  - Cytotoxicity
OT  - Human cells
OT  - Marine organisms
OT  - Tamoxifen
EDAT- 2019/05/31 06:00
MHDA- 2019/10/31 06:00
CRDT- 2019/05/31 06:00
PHST- 2019/01/22 00:00 [received]
PHST- 2019/05/06 00:00 [accepted]
PHST- 2019/05/31 06:00 [pubmed]
PHST- 2019/10/31 06:00 [medline]
PHST- 2019/05/31 06:00 [entrez]
AID - S0160-4120(18)33231-8 [pii]
AID - 10.1016/j.envint.2019.05.014 [doi]
PST - ppublish
SO  - Environ Int. 2019 Aug;129:256-272. doi: 10.1016/j.envint.2019.05.014. Epub 2019 
      May 27.