PMID- 31147466
OWN - NLM
STAT- MEDLINE
DCOM- 20200406
LR  - 20200408
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 116
IP  - 25
DP  - 2019 Jun 18
TI  - ANO1/TMEM16A regulates process maturation in radial glial cells in the developing 
      brain.
PG  - 12494-12499
LID - 10.1073/pnas.1901067116 [doi]
AB  - Neural stem cells (NSCs) are primary progenitor cells in the early developmental 
      stage in the brain that initiate a diverse lineage of differentiated neurons and 
      glia. Radial glial cells (RGCs), a type of neural stem cell in the ventricular 
      zone, are essential for nurturing and delivering new immature neurons to the 
      appropriate cortical target layers. Here we report that Anoctamin 1 
      (ANO1)/TMEM16A, a Ca(2+)-activated chloride channel, mediates the 
      Ca(2+)-dependent process extension of RGCs. ANO1 is highly expressed and 
      functionally active in RGCs of the mouse embryonic ventricular zone. Knockdown of 
      ANO1 suppresses RGC process extension and protrusions, whereas ANO1 
      overexpression stimulates process extension. Among various trophic factors, 
      brain-derived neurotrophic factor (BDNF) activates ANO1, which is required for 
      BDNF-induced process extension in RGCs. More importantly, Ano1-deficient mice 
      exhibited disrupted cortical layers and reduced cortical thickness. We thus 
      conclude that the regulation of RGC process extension by ANO1 contributes to the 
      normal formation of mouse embryonic brain.
CI  - Copyright (c) 2019 the Author(s). Published by PNAS.
FAU - Hong, Gyu-Sang
AU  - Hong GS
AD  - Brain Science Institute, Korea Institute of Science and Technology, 02792 Seoul, 
      Korea.
FAU - Lee, Sung Hoon
AU  - Lee SH
AD  - College of Pharmacy, Chung-ang University, 06974 Seoul, Korea.
FAU - Lee, Byeongjun
AU  - Lee B
AD  - Brain Science Institute, Korea Institute of Science and Technology, 02792 Seoul, 
      Korea.
FAU - Choi, Jae Hyouk
AU  - Choi JH
AD  - Division of Bio-Medical Science and Technology, Korea Institute of Science and 
      Technology, 02792 Seoul, Korea.
FAU - Oh, Soo-Jin
AU  - Oh SJ
AD  - Brain Science Institute, Korea Institute of Science and Technology, 02792 Seoul, 
      Korea.
FAU - Jang, Yongwoo
AU  - Jang Y
AD  - Department of Biomedical Engineering, Hanyang University, 04763 Seoul, Korea.
FAU - Hwang, Eun Mi
AU  - Hwang EM
AD  - Brain Science Institute, Korea Institute of Science and Technology, 02792 Seoul, 
      Korea.
AD  - Division of Bio-Medical Science and Technology, Korea Institute of Science and 
      Technology, 02792 Seoul, Korea.
FAU - Kim, Hyungsup
AU  - Kim H
AD  - Brain Science Institute, Korea Institute of Science and Technology, 02792 Seoul, 
      Korea.
FAU - Jung, Jooyoung
AU  - Jung J
AD  - Brain Science Institute, Korea Institute of Science and Technology, 02792 Seoul, 
      Korea.
FAU - Kim, In-Beom
AU  - Kim IB
AD  - Department of Anatomy, College of Medicine, Catholic University, 06591 Seoul, 
      Korea.
FAU - Oh, Uhtaek
AU  - Oh U
AUID- ORCID: 0000-0002-8935-8863
AD  - Brain Science Institute, Korea Institute of Science and Technology, 02792 Seoul, 
      Korea; utoh@kist.re.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190530
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (ANO1 protein, mouse)
RN  - 0 (Anoctamin-1)
RN  - 0 (Bdnf protein, mouse)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Chlorides)
SB  - IM
MH  - Animals
MH  - Anoctamin-1/genetics/*physiology
MH  - Brain/*cytology/*embryology/metabolism
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Chlorides/metabolism
MH  - Down-Regulation
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Neuroglia/*cytology/metabolism
MH  - Up-Regulation
PMC - PMC6589654
OTO - NOTNLM
OT  - Anoctamin 1
OT  - TMEM16A
OT  - cortical development
OT  - neural stem cell
OT  - radial glial cell
COIS- The authors declare no conflict of interest.
EDAT- 2019/05/31 06:00
MHDA- 2020/04/09 06:00
PMCR- 2019/05/30
CRDT- 2019/06/01 06:00
PHST- 2019/05/31 06:00 [pubmed]
PHST- 2020/04/09 06:00 [medline]
PHST- 2019/06/01 06:00 [entrez]
PHST- 2019/05/30 00:00 [pmc-release]
AID - 1901067116 [pii]
AID - 201901067 [pii]
AID - 10.1073/pnas.1901067116 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2019 Jun 18;116(25):12494-12499. doi: 
      10.1073/pnas.1901067116. Epub 2019 May 30.