PMID- 31147815 OWN - NLM STAT- MEDLINE DCOM- 20190805 LR - 20220409 IS - 1573-5028 (Electronic) IS - 0167-4412 (Linking) VI - 100 IP - 6 DP - 2019 Aug TI - WSL6 encoding an Era-type GTP-binding protein is essential for chloroplast development in rice. PG - 635-645 LID - 10.1007/s11103-019-00885-z [doi] AB - Rice WSL6 is involved in chloroplast ribosome biogenesis and is essential for early chloroplast development. Construction of the genetic translation system is a prerequisite for chloroplast development in plants. However, the molecular mechanism underlying this process is largely unknown. Here, we isolated a white stripe leaf6 (wsl6) mutant in rice. The mutant seedlings displayed white-striped leaves that were more severe under low-temperature conditions. Transmission electron microscopy analysis showed that the wsl6 mutant was defective in early chloroplast development. Map-based cloning revealed that WSL6 encodes an Era-type guanosine-5'-triphosphate (GTP)-binding protein located in chloroplasts. Immunoblotting and quantitative real-time polymerase chain reaction (qRT-PCR) analyses demonstrated an absence of 70S ribosomes in wsl6 chloroplasts. Further research showed that WSL6 binds to the 16S ribosomal RNA (rRNA) subunit of chloroplast ribosome 30S. In summary, these results show that WSL6 is essential for chloroplast ribosome biogenesis during early chloroplast development in rice. FAU - Sun, Yinglun AU - Sun Y AD - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China. FAU - Tian, Yunlu AU - Tian Y AD - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China. FAU - Cheng, Shuhan AU - Cheng S AD - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China. FAU - Wang, Yunlong AU - Wang Y AD - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China. FAU - Hao, Yuanyuan AU - Hao Y AD - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China. FAU - Zhu, Jianping AU - Zhu J AD - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China. FAU - Zhu, Xiaopin AU - Zhu X AD - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China. FAU - Zhang, Yuanyan AU - Zhang Y AD - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China. FAU - Yu, Mingzhou AU - Yu M AD - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China. FAU - Lei, Jie AU - Lei J AD - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China. FAU - Bao, Xiuhao AU - Bao X AD - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China. FAU - Wu, Hongming AU - Wu H AD - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China. FAU - Wang, Yihua AU - Wang Y AD - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China. yihuawang@njau.edu.cn. FAU - Wan, Jianmin AU - Wan J AD - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China. wanjm@njau.edu.cn. AD - National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agriculture Sciences, Beijing, 100081, China. wanjm@njau.edu.cn. LA - eng GR - 2017YFD0100404/National Key R&D Program of China/ GR - 2016ZX08001004/National Key Transformation Program/ PT - Journal Article DEP - 20190530 PL - Netherlands TA - Plant Mol Biol JT - Plant molecular biology JID - 9106343 RN - 0 (Plant Proteins) RN - 0 (RNA, Ribosomal, 16S) RN - EC 3.6.1.- (GTP-Binding Proteins) SB - IM MH - Chloroplasts/*physiology MH - Cloning, Molecular MH - GTP-Binding Proteins/*metabolism/physiology MH - Gene Expression Regulation, Plant MH - Genetic Complementation Test MH - Microscopy, Electron, Transmission MH - Mutation MH - Oryza/*genetics/physiology MH - Phenotype MH - Pigmentation MH - Plant Proteins/*genetics/physiology MH - Protein Biosynthesis MH - RNA, Ribosomal, 16S/genetics MH - Temperature OTO - NOTNLM OT - Era-type GTP-binding protein OT - Oryza sativa OT - PEP OT - Ribosome biogenesis EDAT- 2019/05/31 06:00 MHDA- 2019/08/06 06:00 CRDT- 2019/06/01 06:00 PHST- 2019/01/04 00:00 [received] PHST- 2019/05/18 00:00 [accepted] PHST- 2019/05/31 06:00 [pubmed] PHST- 2019/08/06 06:00 [medline] PHST- 2019/06/01 06:00 [entrez] AID - 10.1007/s11103-019-00885-z [pii] AID - 10.1007/s11103-019-00885-z [doi] PST - ppublish SO - Plant Mol Biol. 2019 Aug;100(6):635-645. doi: 10.1007/s11103-019-00885-z. Epub 2019 May 30.