PMID- 31147911
OWN - NLM
STAT- MEDLINE
DCOM- 20200312
LR  - 20200312
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2004
DP  - 2019
TI  - Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein 
      Interactions in SMC Complexes.
PG  - 79-90
LID - 10.1007/978-1-4939-9520-2_7 [doi]
AB  - Analysis of protein-protein interactions (PPI) is key for the understanding of 
      most protein assemblies including structural maintenance of chromosomes (SMC) 
      complexes. SMC complexes are composed of SMC proteins, kleisin, and 
      kleisin-interacting subunits. These subunits interact in specific ways to 
      constitute and regulate the closed structure of the complexes. Specifically, 
      kleisin molecules bridge the SMC dimers and the kleisin-interacting subunits 
      modulate stability of the bridge. Here we describe a multicomponent version of a 
      yeast two-hybrid (Y2H) method and its application for analysis of the bridging 
      role of the Nse4 kleisin in the SMC5/6 complex. Using this technique, we also 
      show a stabilizing effect of KITE (kleisin-interacting tandem winged-helix 
      element) proteins on SMC5/6.
FAU - Palecek, Jan Josef
AU  - Palecek JJ
AD  - National Centre for Biomolecular Research, Faculty of Science, Masaryk 
      University, Brno, Czech Republic. jpalecek@sci.muni.cz.
AD  - Mendel Centre for Plant Genomics and Proteomics, Central European Institute of 
      Technology, Masaryk University, Brno, Czech Republic. jpalecek@sci.muni.cz.
FAU - Vondrova, Lucie
AU  - Vondrova L
AD  - National Centre for Biomolecular Research, Faculty of Science, Masaryk 
      University, Brno, Czech Republic.
FAU - Zabrady, Katerina
AU  - Zabrady K
AD  - Genome Damage and Stability Centre, University of Sussex, Brighton, UK.
FAU - Otocka, Jakub
AU  - Otocka J
AD  - National Centre for Biomolecular Research, Faculty of Science, Masaryk 
      University, Brno, Czech Republic.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Multiprotein Complexes)
RN  - 0 (Protein Subunits)
RN  - 0 (Saccharomyces cerevisiae Proteins)
SB  - IM
MH  - Cell Cycle Proteins/metabolism
MH  - Chromosomes, Fungal/physiology
MH  - Multiprotein Complexes/*metabolism
MH  - Protein Interaction Maps/*physiology
MH  - Protein Subunits/metabolism
MH  - Saccharomyces cerevisiae/metabolism
MH  - Saccharomyces cerevisiae Proteins/metabolism
MH  - Two-Hybrid System Techniques
OTO - NOTNLM
OT  - KITE proteins
OT  - Kleisin bridge
OT  - Multicomponent yeast two-hybrid (Y2H) system
OT  - Non-SMC Element (Nse) subunits
OT  - Protein-protein interactions
OT  - SMC5/6
OT  - Structural maintenance of chromosomes (SMC) complexes
EDAT- 2019/05/31 06:00
MHDA- 2020/03/13 06:00
CRDT- 2019/06/01 06:00
PHST- 2019/06/01 06:00 [entrez]
PHST- 2019/05/31 06:00 [pubmed]
PHST- 2020/03/13 06:00 [medline]
AID - 10.1007/978-1-4939-9520-2_7 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2019;2004:79-90. doi: 10.1007/978-1-4939-9520-2_7.