PMID- 31147949
OWN - NLM
STAT- MEDLINE
DCOM- 20200122
LR  - 20200122
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1988
DP  - 2019
TI  - Assembly, Intracellular Transport, and Release of MHC Class II Peptide Receptors.
PG  - 297-314
LID - 10.1007/978-1-4939-9450-2_22 [doi]
AB  - MHC class II molecules play a pivotal role for the induction and maintenance of 
      immune responses against pathogens, but are also implicated in pathological 
      conditions like autoimmune diseases or rejection of transplanted organs. Human 
      antigen-presenting cells express three human leukocyte antigen (HLA) class II 
      isotypes (DR, DP, and DQ), which are, with the exception of DRalpha, composed of 
      highly polymorphic alpha and beta subunits. The combination of alpha- and beta-chains results 
      in a multitude of MHC-II alphabeta-heterodimers of the same isotype, but also 
      isotype-mixed MHC class II molecules have been identified. Invariant chain 
      chaperones the assembly of MHC-II molecules within the endoplasmatic reticulum 
      and also facilitates the intracellular transport to MHC class II loading 
      compartments (MIICs). MHC-II molecules are loaded with antigenic peptides and 
      shuttled to the cell surface for inspection by CD4 T-cells. Alternatively, 
      class-II molecules enriched on intraluminal vesicles can be released via exosomes 
      into the extracellular space. Since some of the alphabeta-combinations may yield 
      mismatched nonfunctional heterodimers, it is not entirely clear which type of HLA 
      class II peptide receptors are transported to MIICs and found on the cell surface 
      of antigen-presenting cells. We present techniques to inspect assembly, 
      intracellular transport, cell surface expression, and exosomal release of MHC 
      class II heterodimers.
FAU - Temme, Sebastian
AU  - Temme S
AD  - Department of Molecular Cardiology, Heinrich-Heine-University of Dusseldorf, 
      Dusseldorf, Germany. sebastian.temmme@uni-duesseldorf.de.
FAU - Temme, Nadine
AU  - Temme N
AD  - Division of Immunobiology, Institute of Genetics, University of Bonn, Bonn, 
      Germany.
FAU - Koch, Norbert
AU  - Koch N
AD  - Division of Immunobiology, Institute of Genetics, University of Bonn, Bonn, 
      Germany.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Antigens, Differentiation, B-Lymphocyte)
RN  - 0 (Carbohydrates)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Peptides)
RN  - 0 (Protein Subunits)
RN  - 0 (Receptors, Peptide)
RN  - 0 (invariant chain)
RN  - 368GB5141J (Sodium Dodecyl Sulfate)
RN  - EC 3.2.1.- (Glycoside Hydrolases)
SB  - IM
MH  - Animals
MH  - Antigens, Differentiation, B-Lymphocyte/metabolism
MH  - COS Cells
MH  - Carbohydrates/chemistry
MH  - Cell Membrane/metabolism
MH  - Chlorocebus aethiops
MH  - Endosomes/metabolism
MH  - Exosomes/metabolism
MH  - Glycoside Hydrolases/metabolism
MH  - Histocompatibility Antigens Class II/*metabolism
MH  - Intracellular Space/*metabolism
MH  - Peptides/metabolism
MH  - Protein Subunits/metabolism
MH  - Protein Transport
MH  - Receptors, Peptide/*metabolism
MH  - Sodium Dodecyl Sulfate/metabolism
OTO - NOTNLM
OT  - Antigen presentation
OT  - Endoglycosidase H
OT  - Exosomes
OT  - Flow cytometry
OT  - Intracellular transport
OT  - Invariant chain
OT  - MHC class II molecules
OT  - MIICs
OT  - SDS-stable MHC-II dimers
EDAT- 2019/05/31 06:00
MHDA- 2020/01/23 06:00
CRDT- 2019/06/01 06:00
PHST- 2019/06/01 06:00 [entrez]
PHST- 2019/05/31 06:00 [pubmed]
PHST- 2020/01/23 06:00 [medline]
AID - 10.1007/978-1-4939-9450-2_22 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2019;1988:297-314. doi: 10.1007/978-1-4939-9450-2_22.