PMID- 31149047
OWN - NLM
STAT- MEDLINE
DCOM- 20200629
LR  - 20200629
IS  - 1838-7640 (Electronic)
IS  - 1838-7640 (Linking)
VI  - 9
IP  - 8
DP  - 2019
TI  - Biodistribution of gadolinium- and near infrared-labeled human umbilical cord 
      mesenchymal stromal cell-derived exosomes in tumor bearing mice.
PG  - 2325-2345
LID - 10.7150/thno.30030 [doi]
AB  - We speculate that exosomes derived from human umbilical cord mesenchymal stromal 
      cells (HUC-MSCs) will accumulate within tumors and have the potential for both 
      tumor location or drug delivery. Methods: To determine proof of concept, HUC-MSC 
      exosomes were labeled with an MRI contrast agent, gadolinium, or a near infrared 
      dye. Exosome accumulation within ectopic osteosarcoma tumor-bearing mice was 
      determined by 14.1 T MRI or bioimaging over 24-48 h after injection. In vitro 
      studies examine the accumulation and physiological effect of exosomes on human 
      and mouse osteosarcoma cell lines by MTT assay, confocal microscopy, and flow 
      cytometry. Results: Systemic HUC-MSC exosomes accumulated continuously in tumor 
      over a 24-48 h post-injection period. In contrast, synthetic lipid nanoparticles 
      accumulate in tumor only for the first 3 h post-injection. Conclusion: These 
      results suggest that HUC-MSCs exosomes accumulate within human or mouse 
      osteosarcoma cells in vitro and in vivo over a 24 to 48 h after infusion.
FAU - Abello, Javier
AU  - Abello J
AD  - Department of Anatomy and Physiology, Kansas State University, Manhattan, KS 
      66506, USA.
AD  - Department of Food, Nutrition, Dietetics and Health, Kansas State University, 
      Manhattan, KS 66506, USA.
FAU - Nguyen, Tuyen Duong Thanh
AU  - Nguyen TDT
AD  - Department of Chemistry, Kansas State University, Manhattan, KS 66506, USA.
AD  - Nanotechnology Innovation Center of Kansas State (NICKS), Kansas State 
      University, Manhattan, KS 66506, USA.
FAU - Marasini, Ramesh
AU  - Marasini R
AD  - Department of Chemistry, Kansas State University, Manhattan, KS 66506, USA.
AD  - Nanotechnology Innovation Center of Kansas State (NICKS), Kansas State 
      University, Manhattan, KS 66506, USA.
FAU - Aryal, Santosh
AU  - Aryal S
AD  - Department of Chemistry, Kansas State University, Manhattan, KS 66506, USA.
AD  - Nanotechnology Innovation Center of Kansas State (NICKS), Kansas State 
      University, Manhattan, KS 66506, USA.
FAU - Weiss, Mark Louis
AU  - Weiss ML
AD  - Department of Anatomy and Physiology, Kansas State University, Manhattan, KS 
      66506, USA.
AD  - Midwest Institute of Comparative Stem Cell Biology, Kansas State University, 
      Manhattan, KS 66506, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190412
PL  - Australia
TA  - Theranostics
JT  - Theranostics
JID - 101552395
RN  - AU0V1LM3JT (Gadolinium)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Cells, Cultured
MH  - Exosomes/chemistry/metabolism/*transplantation
MH  - Gadolinium/chemistry/*pharmacokinetics
MH  - Humans
MH  - Infrared Rays
MH  - Magnetic Resonance Imaging/methods
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Mice
MH  - Mice, Nude
MH  - Nanoparticles/chemistry/metabolism
MH  - Optical Imaging/methods
MH  - Osteosarcoma/*diagnostic imaging
MH  - Tissue Distribution
MH  - Umbilical Cord/cytology
PMC - PMC6531310
OTO - NOTNLM
OT  - Extracellular vesicles
OT  - ectopic osteosarcoma model
OT  - gadolinium nanoparticle.
OT  - non-invasive imaging
COIS- Competing Interests: The authors have declared that no competing interest exists.
EDAT- 2019/06/01 06:00
MHDA- 2020/07/01 06:00
PMCR- 2019/01/01
CRDT- 2019/06/01 06:00
PHST- 2018/09/17 00:00 [received]
PHST- 2019/02/11 00:00 [accepted]
PHST- 2019/06/01 06:00 [entrez]
PHST- 2019/06/01 06:00 [pubmed]
PHST- 2020/07/01 06:00 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - thnov09p2325 [pii]
AID - 10.7150/thno.30030 [doi]
PST - epublish
SO  - Theranostics. 2019 Apr 12;9(8):2325-2345. doi: 10.7150/thno.30030. eCollection 
      2019.