PMID- 31149811
OWN - NLM
STAT- MEDLINE
DCOM- 20200925
LR  - 20200925
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 91
IP  - 14
DP  - 2019 Jul 16
TI  - Investigating the Potential of Ion Mobility-Mass Spectrometry for Microalgae 
      Biomass Characterization.
PG  - 9266-9276
LID - 10.1021/acs.analchem.9b02172 [doi]
AB  - Algae biomass is formed by an extremely complex set of metabolites, and its 
      molecular characterization has been very challenging. We report the 
      characterization of microalgae extracts via traveling wave ion mobility-mass 
      spectrometry (TWIM-MS) by two different analysis strategies. First, the extracts 
      were analyzed by direct infusion electrospray ionization (ESI) with no previous 
      chromatographic separation (DI-ESI-TWIM-MS). Second, the samples were screened 
      for metabolites and lipids using an untargeted high-throughput method that 
      employs ultrahigh-performance liquid chromatography (UHPLC) using 
      data-independent analysis (DIA) - MS(E) (UHPLC-HDMS(E)). Sixteen different 
      microalgae biomasses were evaluated by both strategies. DI-ESI-TWIM-MS was able, 
      via distinct drift times, to set apart different classes of metabolites, with the 
      differences in the profiles of each microalga readily evident. With the 
      UHPLC-HDMS(E) approach, 1251 different compounds were putatively annotated across 
      16 samples with 210 classified as lipids. From the normalized abundance for each 
      annotated compound category, a detailed profiling in terms of metabolites, 
      lipids, and lipid classes of each sample was performed. The reported workflow 
      represents a powerful tool to determine the most suitable biotechnological 
      applications for a given alga type and may allow for real-time monitoring of the 
      algae composition distribution as a function of growth conditions, feedstocks, 
      and the like. The determination of collision cross section results in improved 
      confidence in the identification of triacylglycerols in samples, highly 
      applicable to biofuels production. The two analysis strategies explored in this 
      work offer powerful tools for the biomass industry by aiding in the 
      identification of ideal strains and culture conditions for a specific 
      application, saving analysis time and facilitating identification of a large 
      number of constituents at once.
FAU - Fasciotti, Maira
AU  - Fasciotti M
AUID- ORCID: 0000-0002-6358-6067
AD  - National Institute of Metrology, Quality and Technology (INMETRO), Division of 
      Chemical and Thermal Metrology , Laboratory of Organic Analysis , 25250-020 , 
      Duque de Caxias , Rio de Janeiro , Brazil.
AD  - ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry , University of 
      Campinas - UNICAMP , 13083-970 Campinas , Sao Paulo , Brazil.
FAU - Souza, Gustavo H M F
AU  - Souza GHMF
AD  - MS Applications and Development Laboratory , Waters Corporation , 06455-000 
      Barueri , Sao Paulo , Brazil.
FAU - Astarita, Giuseppe
AU  - Astarita G
AD  - Department of Biochemistry and Molecular and Cellular Biology , Georgetown 
      University , Washington DC 20007 , United States.
FAU - Costa, Ingrid C R
AU  - Costa ICR
AD  - National Institute of Metrology, Quality and Technology (INMETRO), Division of 
      Chemical and Thermal Metrology , Laboratory of Organic Analysis , 25250-020 , 
      Duque de Caxias , Rio de Janeiro , Brazil.
FAU - Monteiro, Thays V C
AU  - Monteiro TVC
AD  - National Institute of Metrology, Quality and Technology (INMETRO), Division of 
      Chemical and Thermal Metrology , Laboratory of Organic Analysis , 25250-020 , 
      Duque de Caxias , Rio de Janeiro , Brazil.
FAU - Teixeira, Claudia M L L
AU  - Teixeira CMLL
AD  - Microalgal Biotechnology Laboratory , National Institute of Technology (INT) , 
      20081-312 Rio de Janeiro , Rio de Janeiro , Brazil.
FAU - Eberlin, Marcos N
AU  - Eberlin MN
AD  - Mackenzie Presbyterian University , School of Engineering , 01302-907 Sao Paulo , 
      Sao Paulo , Brazil.
FAU - Sarpal, Amarijt S
AU  - Sarpal AS
AD  - National Institute of Metrology, Quality and Technology (INMETRO), Division of 
      Chemical and Thermal Metrology , Laboratory of Organic Analysis , 25250-020 , 
      Duque de Caxias , Rio de Janeiro , Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190624
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Lipids)
SB  - IM
MH  - *Biomass
MH  - Chromatography, High Pressure Liquid
MH  - Ion Mobility Spectrometry/*methods
MH  - Lipidomics/methods
MH  - Lipids/*analysis
MH  - *Metabolome
MH  - Metabolomics/methods
MH  - Microalgae/*chemistry
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
EDAT- 2019/06/01 06:00
MHDA- 2020/09/26 06:00
CRDT- 2019/06/01 06:00
PHST- 2019/06/01 06:00 [pubmed]
PHST- 2020/09/26 06:00 [medline]
PHST- 2019/06/01 06:00 [entrez]
AID - 10.1021/acs.analchem.9b02172 [doi]
PST - ppublish
SO  - Anal Chem. 2019 Jul 16;91(14):9266-9276. doi: 10.1021/acs.analchem.9b02172. Epub 
      2019 Jun 24.