PMID- 31151290
OWN - NLM
STAT- MEDLINE
DCOM- 20191125
LR  - 20200225
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 24
IP  - 11
DP  - 2019 May 30
TI  - Anti-Candida Biofilm Activity of Pterostilbene or Crude Extract from 
      Non-Fermented Grape Pomace Entrapped in Biopolymeric Nanoparticles.
LID - 10.3390/molecules24112070 [doi]
LID - 2070
AB  - Polymeric nanoparticle-based carriers are promising agents to deliver drugs to 
      cells. Vitis vinifera phenolic compounds are known for their antifungal activity 
      against Candida albicans. The aim of the present study was to investigate the 
      antifungal activity of pterostilbene or crude extracts from non-fermented grape 
      pomace, entrapped in poly(lactic-co-glycolic) acid nanoparticles (NPs), with 
      diameters of 50 and 150 nm, on Candida biofilm. The fluorescent probe coumarin 6 
      was used to study the uptake of poly(lactic-co-glycolic)acid (PLGA) NPs in 
      planktonic cells and biofilm. The green fluorescent signal of coumarin 6 was 
      observed in Candida biofilm after 24 and 48 hours. Both pterostilbene and crude 
      pomace extract entrapped in NPs exerted a significantly higher anti-biofilm 
      activity compared to their free forms. The entrapment efficiency of both 
      pterostilbene and crude pomace extract in PLGA NPs was ~90%. At 16 microg/mL, 
      pterostilbene loaded in PLGA NPs reduced biofilm formation of 63% and reduced 
      mature biofilm of 50%. Moreover, at 50 microg/mL, the pomace extract loaded in NPs 
      reduced mature biofilm of 37%. These results strongly suggest that PLGA NPs are 
      promising nanodevices for the delivery of antifungal drugs as the crude grape 
      pomace extract, a by-product of white wine making.
FAU - Simonetti, Giovanna
AU  - Simonetti G
AD  - Department of Public Health and Infectious Diseases "Sapienza" University of 
      Rome, P. le Aldo Moro 5, 00185 Rome, Italy. giovanna.simonetti@uniroma1.it.
FAU - Palocci, Cleofe
AU  - Palocci C
AD  - Department of Chemistry "Sapienza" University of Rome, 00185 Rome, Italy. 
      cleofe.palocci@uniroma1.it.
FAU - Valletta, Alessio
AU  - Valletta A
AD  - Department of Environmental Biology "Sapienza" University of Rome, 00185 Rome, 
      Italy. alessio.valletta@uniroma1.it.
FAU - Kolesova, Olga
AU  - Kolesova O
AD  - Department of Public Health and Infectious Diseases "Sapienza" University of 
      Rome, P. le Aldo Moro 5, 00185 Rome, Italy. olga.kolesova@uniroma1.it.
FAU - Chronopoulou, Laura
AU  - Chronopoulou L
AD  - Department of Chemistry "Sapienza" University of Rome, 00185 Rome, Italy. 
      laura.chronopoulou@uniroma1.it.
FAU - Donati, Livia
AU  - Donati L
AD  - Department of Environmental Biology "Sapienza" University of Rome, 00185 Rome, 
      Italy. donatilivia@gmail.com.
FAU - Di Nitto, Antonio
AU  - Di Nitto A
AD  - Department of Chemistry "Sapienza" University of Rome, 00185 Rome, Italy. 
      antonio.dinitto@uniroma1.it.
FAU - Brasili, Elisa
AU  - Brasili E
AD  - Department of Environmental Biology "Sapienza" University of Rome, 00185 Rome, 
      Italy. elisa.brasili@uniroma1.it.
FAU - Tomai, Pierpaolo
AU  - Tomai P
AD  - Department of Chemistry "Sapienza" University of Rome, 00185 Rome, Italy. 
      pierpaolo.tomai@uniroma1.it.
FAU - Gentili, Alessandra
AU  - Gentili A
AD  - Department of Chemistry "Sapienza" University of Rome, 00185 Rome, Italy. 
      alessandra.gentili@uniroma1.it.
FAU - Pasqua, Gabriella
AU  - Pasqua G
AD  - Department of Environmental Biology "Sapienza" University of Rome, 00185 Rome, 
      Italy. gabriella.pasqua@uniroma1.it.
LA  - eng
GR  - C26A14RP98/University Sapienza of Rome/
PT  - Journal Article
DEP - 20190530
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Antifungal Agents)
RN  - 0 (Biopolymers)
RN  - 0 (Plant Extracts)
RN  - 0 (Stilbenes)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26R60S6A5I (pterostilbene)
SB  - IM
MH  - Antifungal Agents/chemistry/*pharmacology
MH  - Biofilms/*drug effects
MH  - Biopolymers/chemistry
MH  - Candida/*drug effects
MH  - Chemical Phenomena
MH  - *Nanoparticles/chemistry
MH  - Plant Extracts/chemistry/*pharmacology
MH  - Polylactic Acid-Polyglycolic Acid Copolymer/chemistry
MH  - Stilbenes/chemistry/*pharmacology
MH  - Vitis/chemistry
PMC - PMC6600237
OTO - NOTNLM
OT  - Candida albicans biofilm
OT  - Vitis vinifera
OT  - antifungal activity
OT  - biopolymeric nanoparticles
OT  - crude grape pomace extract
OT  - pterostilbene
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could lead to a potential conflict of 
      interest.
EDAT- 2019/06/04 06:00
MHDA- 2019/11/26 06:00
PMCR- 2019/05/30
CRDT- 2019/06/02 06:00
PHST- 2019/04/17 00:00 [received]
PHST- 2019/05/19 00:00 [revised]
PHST- 2019/05/28 00:00 [accepted]
PHST- 2019/06/02 06:00 [entrez]
PHST- 2019/06/04 06:00 [pubmed]
PHST- 2019/11/26 06:00 [medline]
PHST- 2019/05/30 00:00 [pmc-release]
AID - molecules24112070 [pii]
AID - molecules-24-02070 [pii]
AID - 10.3390/molecules24112070 [doi]
PST - epublish
SO  - Molecules. 2019 May 30;24(11):2070. doi: 10.3390/molecules24112070.