PMID- 31152512
OWN - NLM
STAT- MEDLINE
DCOM- 20190731
LR  - 20190731
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Linking)
VI  - 37
IP  - 6
DP  - 2019 Jun 8
TI  - [Simultaneous determination of nine artificial sweeteners in food by solid-phase 
      extraction coupled with high performance liquid chromatography-tandem mass 
      spectrometry].
PG  - 619-625
LID - 10.3724/SP.J.1123.2019.01012 [doi]
AB  - A simple and rapid method based on solid-phase extraction and high performance 
      liquid chromatography-tandem mass spectrometry for the simultaneous determination 
      of nine artificial sweeteners (acesulfame-K, saccharin sodium, cyclamate, 
      sucralose, aspartame, alitame, neotame, dulcin, neohesperidin dihydrochalcone) in 
      various foods was developed. The sweeteners in food samples were extracted with 
      triethylamine buffer solution (pH 4.5) and cleaned using an SPE column equipped 
      with hydrophilic and lipophilic packing material. The analytes were separated on 
      a Phenomenex Knietex((R)) F5 column (100 mmx2.1 mm, 2.6 mum) using 0.1% (v/v) formic 
      acid-5 mmol/L ammonium formate/methanol as a mobile phase for gradient elution, 
      and then determined by tandem mass spectrometry in positive and negative ESI 
      modes under multiple reaction monitoring (MRM). The internal standard method was 
      used to further suppress the matrix effect. The method proved to be very 
      effective in the removal of matrix interferences. Calibration curves were linear 
      within a studied range of concentrations (r(2)>0.999) for the nine artificial 
      sweeteners. The limits of detection (LODs) and limits of quantification (LOQs) 
      were within 2-30 mug/kg and 6-100 mug/kg, respectively. The recoveries for the nine 
      investigated sweeteners were within 86.3%-106.3% at three spiked levels, with 
      relative standard deviations (RSDs) between 1.2% and 5.9%. The developed method 
      is rapid, efficient, accurate, and reliable; it can also be applied for the rapid 
      determination of other artificial sweeteners in a complex food matrix.
FAU - Tang, Jiwang
AU  - Tang J
AD  - Hunan Testing Institute of Product and Commodity Supervison, Changsha 410007, 
      China.
AD  - The chemical and chemical engineering academy of central south university, 
      Changsha 410083, China.
FAU - Yuan, Liejiang
AU  - Yuan L
AD  - Hunan Testing Institute of Product and Commodity Supervison, Changsha 410007, 
      China.
FAU - Xiao, Yong
AU  - Xiao Y
AD  - Hunan Testing Institute of Product and Commodity Supervison, Changsha 410007, 
      China.
FAU - Wang, Xiu
AU  - Wang X
AD  - Hunan Testing Institute of Product and Commodity Supervison, Changsha 410007, 
      China.
FAU - Wang, Shuxia
AU  - Wang S
AD  - Hunan Testing Institute of Product and Commodity Supervison, Changsha 410007, 
      China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Non-Nutritive Sweeteners)
RN  - 0 (Sweetening Agents)
MH  - Chromatography, High Pressure Liquid
MH  - Non-Nutritive Sweeteners/*analysis
MH  - Solid Phase Extraction
MH  - Sweetening Agents/*analysis
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - artificial sweetener
OT  - food
OT  - high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)
OT  - solid-phaseextraction (SPE)
EDAT- 2019/06/04 06:00
MHDA- 2019/08/01 06:00
CRDT- 2019/06/02 06:00
PHST- 2019/06/02 06:00 [entrez]
PHST- 2019/06/04 06:00 [pubmed]
PHST- 2019/08/01 06:00 [medline]
AID - 10.3724/SP.J.1123.2019.01012 [doi]
PST - ppublish
SO  - Se Pu. 2019 Jun 8;37(6):619-625. doi: 10.3724/SP.J.1123.2019.01012.