PMID- 31155175
OWN - NLM
STAT- MEDLINE
DCOM- 20190715
LR  - 20190715
IS  - 1873-2623 (Electronic)
IS  - 0041-1345 (Linking)
VI  - 51
IP  - 5
DP  - 2019 Jun
TI  - Detection of Intragraft Anti-Blood Group A and B Antibodies Following Renal 
      Transplantation.
PG  - 1371-1377
LID - S0041-1345(18)31440-4 [pii]
LID - 10.1016/j.transproceed.2019.01.128 [doi]
AB  - BACKGROUND: Graft immunocomplex capture fluorescence analysis is an attractive 
      method to detect intragraft donor-specific anti-HLA antibodies. In 
      ABO-incompatible transplantation, anti-A and B antibodies are also considered to 
      be important donor specific antibodies (ABO-DSA). Therefore, it is useful to 
      monitor intragraft ABO-DSAs to assess antibody-mediated rejection. METHODS: To 
      capture A and B antigens, anti-Band III, von Willebrand factor (VW), and 
      plasmalemma vesicle-associated protein (PLVAP) beads were produced. The allograft 
      specimen was homogenized in a lysis buffer. Subsequently, A and B antigens were 
      captured by anti-Band III, VW, or PLVAP beads. The immune complexes were then 
      detected by phycoerythrin-conjugated anti-human IgG antibodies and analyzed using 
      a Luminex system. RESULTS: Although Band III and VW beads yielded false positives 
      and false negatives, PLVAP beads captured A and B antigens with high sensitivity 
      (91.7%) and specificity (100%) when an index > 1.5 was considered positive. The 
      proximity in A and B antigens and PLVAP expression was confirmed using 
      immunohistochemical evaluation. Furthermore, sodium dodecyl sulfate 
      polyacrylamide gel electrophoresis supported that PLVAP is an A and B antigen 
      carrier protein. CASE REPORT: Biopsies were conducted following an 
      ABO-incompatible renal transplant (type A to O) and evaluated for ABO-DSA. Graft 
      immunocomplex capture fluorescence analysis was demonstrated as follows: 3.19 (1 
      h, serum creatinine [s-Cr] 3.95 mg/dL, titer IgG 1:512, glomerulitis [g] 0, 
      peritubular capillaritis [ptc] 0, complement 4d [C4d] 1); 1.8 (4 d, s-Cr 2.29 
      mg/dL, titer 1:256, g 0, ptc 0, C4d 3); 1.2 (22 d, s-Cr 1.58 mg/dL, titer 1:128, 
      g 0, ptc 2, C4d 3). This result indicated that the remnant ABO-DSA were adsorbed 
      and subsequently removed from the allograft successfully. CONCLUSIONS: This novel 
      application could be used to detect intragraft ABO-DSAs, which could lead to a 
      correct diagnosis and shed light on the ABO-DSA kinetics following 
      ABO-incompatible transplantation.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Nakamura, Tsukasa
AU  - Nakamura T
AD  - Department of Organ Transplantation and General Surgery, Kyoto Prefectural 
      University of Medicine, Kajii-cho 465, Kamigyo-ku, Kyoto-prefecture, Japan. 
      Electronic address: tsukasa@koto.kpu-m.ac.jp.
FAU - Shirouzu, Takayuki
AU  - Shirouzu T
AD  - Wakunaga Pharmaceutical Co, Ltd, Molecular Diagnostics Division, Osaka, Japan.
FAU - Kawai, Shintaro
AU  - Kawai S
AD  - Wakunaga Pharmaceutical Co, Ltd, Molecular Diagnostics Division, Osaka, Japan.
FAU - Imanishi, Yui
AU  - Imanishi Y
AD  - Department of Blood Transfusion and Cell Therapy, Kyoto Prefectural University of 
      Medicine, Kyoto, Japan.
FAU - Matsuyama, Takehisa
AU  - Matsuyama T
AD  - Department of Organ Transplantation and General Surgery, Kyoto Prefectural 
      University of Medicine, Kajii-cho 465, Kamigyo-ku, Kyoto-prefecture, Japan.
FAU - Harada, Shumpei
AU  - Harada S
AD  - Department of Organ Transplantation and General Surgery, Kyoto Prefectural 
      University of Medicine, Kajii-cho 465, Kamigyo-ku, Kyoto-prefecture, Japan.
FAU - Nobori, Shuji
AU  - Nobori S
AD  - Department of Organ Transplantation and General Surgery, Kyoto Prefectural 
      University of Medicine, Kajii-cho 465, Kamigyo-ku, Kyoto-prefecture, Japan.
FAU - Yoshimura, Norio
AU  - Yoshimura N
AD  - Department of Organ Transplantation and General Surgery, Kyoto Prefectural 
      University of Medicine, Kajii-cho 465, Kamigyo-ku, Kyoto-prefecture, Japan.
FAU - Ushigome, Hidetaka
AU  - Ushigome H
AD  - Department of Organ Transplantation and General Surgery, Kyoto Prefectural 
      University of Medicine, Kajii-cho 465, Kamigyo-ku, Kyoto-prefecture, Japan.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
RN  - 0 (Blood Group Antigens)
RN  - 0 (HLA Antigens)
RN  - 0 (Isoantibodies)
SB  - IM
MH  - Adult
MH  - Biopsy
MH  - Blood Group Antigens/*analysis
MH  - Female
MH  - Fluorescent Antibody Technique/*methods
MH  - Graft Rejection/*immunology
MH  - HLA Antigens/immunology
MH  - Humans
MH  - Isoantibodies/*analysis
MH  - *Kidney Transplantation
MH  - Male
MH  - Tissue Donors
MH  - Transplantation, Homologous
EDAT- 2019/06/04 06:00
MHDA- 2019/07/16 06:00
CRDT- 2019/06/04 06:00
PHST- 2018/11/08 00:00 [received]
PHST- 2019/01/04 00:00 [accepted]
PHST- 2019/06/04 06:00 [entrez]
PHST- 2019/06/04 06:00 [pubmed]
PHST- 2019/07/16 06:00 [medline]
AID - S0041-1345(18)31440-4 [pii]
AID - 10.1016/j.transproceed.2019.01.128 [doi]
PST - ppublish
SO  - Transplant Proc. 2019 Jun;51(5):1371-1377. doi: 
      10.1016/j.transproceed.2019.01.128.