PMID- 31160568
OWN - NLM
STAT- MEDLINE
DCOM- 20190624
LR  - 20210109
IS  - 2041-1723 (Electronic)
IS  - 2041-1723 (Linking)
VI  - 10
IP  - 1
DP  - 2019 Jun 3
TI  - A comprehensive single cell transcriptional landscape of human hematopoietic 
      progenitors.
PG  - 2395
LID - 10.1038/s41467-019-10291-0 [doi]
LID - 2395
AB  - Hematopoietic Stem/Progenitor cells (HSPCs) are endowed with the role of 
      maintaining a diverse pool of blood cells throughout the human life. Despite 
      recent efforts, the nature of the early cell fate decisions remains contentious. 
      Using single-cell RNA-Seq, we show that existing approaches to stratify bone 
      marrow CD34+ cells reveal a hierarchically-structured transcriptional landscape 
      of hematopoietic differentiation. Still, this landscape misses important early 
      fate decisions. We here provide a broader transcriptional profiling of bone 
      marrow lineage negative hematopoietic progenitors that recovers a key missing 
      branchpoint into basophils and expands our understanding of the underlying 
      structure of early adult human haematopoiesis. We also show that this map has 
      strong similarities in topology and gene expression to that found in mouse. 
      Finally, we identify the sialomucin CD164, as a reliable marker for the earliest 
      branches of HSPCs specification and we showed how its use can foster the design 
      of alternative transplantation cell products.
FAU - Pellin, Danilo
AU  - Pellin D
AD  - Gene Therapy Program, Dana-Farber/Boston Children's Cancer and Blood Disorders 
      Center, Harvard Medical School, Boston, MA, 02115, USA.
FAU - Loperfido, Mariana
AU  - Loperfido M
AUID- ORCID: 0000-0001-9365-5388
AD  - Gene Therapy Program, Dana-Farber/Boston Children's Cancer and Blood Disorders 
      Center, Harvard Medical School, Boston, MA, 02115, USA.
FAU - Baricordi, Cristina
AU  - Baricordi C
AD  - Gene Therapy Program, Dana-Farber/Boston Children's Cancer and Blood Disorders 
      Center, Harvard Medical School, Boston, MA, 02115, USA.
FAU - Wolock, Samuel L
AU  - Wolock SL
AUID- ORCID: 0000-0002-2120-4788
AD  - Department of Systems Biology, Harvard Medical School, Boston, MA, 02115, USA.
FAU - Montepeloso, Annita
AU  - Montepeloso A
AD  - Gene Therapy Program, Dana-Farber/Boston Children's Cancer and Blood Disorders 
      Center, Harvard Medical School, Boston, MA, 02115, USA.
FAU - Weinberg, Olga K
AU  - Weinberg OK
AD  - Department of Pathology, Boston Children's Hospital and Harvard Medical School, 
      Boston, MA, 02115, USA.
FAU - Biffi, Alessandra
AU  - Biffi A
AD  - Gene Therapy Program, Dana-Farber/Boston Children's Cancer and Blood Disorders 
      Center, Harvard Medical School, Boston, MA, 02115, USA.
FAU - Klein, Allon M
AU  - Klein AM
AUID- ORCID: 0000-0001-8913-7879
AD  - Department of Systems Biology, Harvard Medical School, Boston, MA, 02115, USA. 
      Allon_Klein@hms.harvard.edu.
FAU - Biasco, Luca
AU  - Biasco L
AD  - Gene Therapy Program, Dana-Farber/Boston Children's Cancer and Blood Disorders 
      Center, Harvard Medical School, Boston, MA, 02115, USA. l.biasco@ucl.ac.uk.
AD  - University College of London (UCL), Great Ormond Street Institute of Child Health 
      Faculty of Population Health Sciences, London, WC1N 1EH, UK. l.biasco@ucl.ac.uk.
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20190603
PL  - England
TA  - Nat Commun
JT  - Nature communications
JID - 101528555
RN  - 0 (Antigens, CD34)
RN  - 0 (CD164 protein, human)
RN  - 0 (Endolyn)
SB  - IM
MH  - Animals
MH  - Antigens, CD34/metabolism
MH  - Bone Marrow Cells
MH  - Cell Lineage
MH  - Endolyn/metabolism
MH  - Gene Expression Profiling
MH  - Hematopoiesis/*genetics
MH  - Hematopoietic Stem Cells/*metabolism
MH  - Humans
MH  - Mice
MH  - Sequence Analysis, RNA
MH  - Single-Cell Analysis
PMC - PMC6546699
COIS- The authors declare no competing interests.
EDAT- 2019/06/05 06:00
MHDA- 2019/06/25 06:00
PMCR- 2019/06/03
CRDT- 2019/06/05 06:00
PHST- 2019/01/11 00:00 [received]
PHST- 2019/05/03 00:00 [accepted]
PHST- 2019/06/05 06:00 [entrez]
PHST- 2019/06/05 06:00 [pubmed]
PHST- 2019/06/25 06:00 [medline]
PHST- 2019/06/03 00:00 [pmc-release]
AID - 10.1038/s41467-019-10291-0 [pii]
AID - 10291 [pii]
AID - 10.1038/s41467-019-10291-0 [doi]
PST - epublish
SO  - Nat Commun. 2019 Jun 3;10(1):2395. doi: 10.1038/s41467-019-10291-0.