PMID- 31160696
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20200408
LR  - 20210109
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Jun 3
TI  - Amino-isocyanoacridines: Novel, Tunable Solvatochromic Fluorophores as 
      Physiological pH Probes.
PG  - 8250
LID - 10.1038/s41598-019-44760-9 [doi]
LID - 8250
AB  - Amino-isocyanoacridines (ICAAcs), as first members of their class, turned out to 
      be a novel, multifunctional acridine orange (AO) type dye family with a number of 
      additional favorable properties. They have enhanced solvatochromic emission 
      range, low quantum yields (Phi(F) = 2.9-0.4%) in water, reduced basicity 
      (pK(a) = 7.05-7.58), and their optical behavior could be fine-tuned by 
      complexation with Ag(I) ions, too. Based on both their vibronic absorption and 
      the charge transfer bands, ICAAcs can be applied as stable pH-probes with great 
      precision (2-3% error) in the physiological pH range of 6-8 using UV-vis and 
      fluorescence detection. The dyes are also able to sense pH change in different 
      microenvironments, such as the Stern layer, as it was demonstrated on sodium 
      lauryl sulfate micelles. The optical behavior of the ICAAc derivatives is 
      discussed based on high-level quantum chemical calculations. All three dyes are 
      well-applicable with conventional epifluorescence imaging. Furthermore, at the 
      blue excitation, diMICAAc is optimally suited as a whole-cell probe for both the 
      conventional microscopic and the laser-illumination studies, like flow- and 
      imaging cytometric, or confocal laser-scanning microscopic examinations.
FAU - Nagy, Miklos
AU  - Nagy M
AD  - Department of Applied Chemistry, University of Debrecen, 4010, Debrecen, Hungary.
FAU - Racz, David
AU  - Racz D
AD  - Department of Applied Chemistry, University of Debrecen, 4010, Debrecen, Hungary.
FAU - Nagy, Zsolt Laszlo
AU  - Nagy ZL
AD  - Department of Applied Chemistry, University of Debrecen, 4010, Debrecen, Hungary.
FAU - Feher, Peter Pal
AU  - Feher PP
AD  - Research Centre for Natural Sciences, Hungarian Academy of Sciences, H-1117 
      Budapest, Magyar tudosok korutja 2, Budapest, Hungary.
FAU - Kovacs, Sandor Lajos
AU  - Kovacs SL
AD  - Department of Applied Chemistry, University of Debrecen, 4010, Debrecen, Hungary.
FAU - Banko, Csaba
AU  - Banko C
AD  - University of Debrecen, Medical and Health Science Center, Faculty of Medicine, 
      Department of Biophysics and Cell Biology, 4010, Debrecen, Hungary.
FAU - Bacso, Zsolt
AU  - Bacso Z
AD  - University of Debrecen, Medical and Health Science Center, Faculty of Medicine, 
      Department of Biophysics and Cell Biology, 4010, Debrecen, Hungary.
FAU - Kiss, Alexandra
AU  - Kiss A
AD  - Department of Biotechnology and Microbiology, Faculty of Science, University of 
      Debrecen, Debrecen, 4010, Hungary.
FAU - Zsuga, Miklos
AU  - Zsuga M
AD  - Department of Applied Chemistry, University of Debrecen, 4010, Debrecen, Hungary.
FAU - Keki, Sandor
AU  - Keki S
AD  - Department of Applied Chemistry, University of Debrecen, 4010, Debrecen, Hungary. 
      keki.sandor@science.unideb.hu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190603
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
PMC - PMC6547668
COIS- The authors declare no competing interests.
EDAT- 2019/06/05 06:00
MHDA- 2019/06/05 06:01
PMCR- 2019/06/03
CRDT- 2019/06/05 06:00
PHST- 2018/12/06 00:00 [received]
PHST- 2019/05/23 00:00 [accepted]
PHST- 2019/06/05 06:00 [entrez]
PHST- 2019/06/05 06:00 [pubmed]
PHST- 2019/06/05 06:01 [medline]
PHST- 2019/06/03 00:00 [pmc-release]
AID - 10.1038/s41598-019-44760-9 [pii]
AID - 44760 [pii]
AID - 10.1038/s41598-019-44760-9 [doi]
PST - epublish
SO  - Sci Rep. 2019 Jun 3;9(1):8250. doi: 10.1038/s41598-019-44760-9.