PMID- 31160748
OWN - NLM
STAT- MEDLINE
DCOM- 20201016
LR  - 20210109
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Jun 3
TI  - Genetic variability of porcine pegivirus in pigs from Europe and China and 
      insights into tissue tropism.
PG  - 8174
LID - 10.1038/s41598-019-44642-0 [doi]
LID - 8174
AB  - Pegiviruses belong to the family Flaviviridae and have been found in humans and 
      other mammalian species. To date eleven different pegivirus species (Pegivirus 
      A-K) have been described. However, little is known about the tissue tropism and 
      replication of pegiviruses. In 2016, a so far unknown porcine pegivirus (PPgV, 
      Pegivirus K) was described and persistent infection in the host, similar to human 
      pegivirus, was reported. In this study, qRT-PCR, phylogenetic analyses and 
      fluorescence in situ hybridization (FISH) were implemented to detect and quantify 
      PPgV genome content in serum samples from domestic pigs from Europe and Asia, in 
      tissue and peripheral blood mononuclear cell (PBMC) samples and wild boar serum 
      samples from Germany. PPgV was detectable in 2.7% of investigated domestic pigs 
      from Europe and China (viral genome load 2.4 x 10(2) to 2.0 x 10(6) PPgV 
      copies/ml), while all wild boar samples were tested negative. Phylogenetic 
      analyses revealed pairwise nucleotide identities >90% among PPgVs. Finally, PPgV 
      was detected in liver, thymus and PBMCs by qRT-PCR and FISH, suggesting liver- 
      and lymphotropism. Taken together, this study provides first insights into the 
      tissue tropism of PPgV and shows its distribution and genetic variability in 
      Europe and China.
FAU - Kennedy, Johanna
AU  - Kennedy J
AD  - Institute of Virology, University of Veterinary Medicine Hannover, 30559, 
      Hannover, Germany.
AD  - German Center for Infection Research (DZIF), Partner Site Hannover-Braunschweig, 
      30559, Hannover, Germany.
FAU - Pfankuche, Vanessa Maria
AU  - Pfankuche VM
AD  - Department of Pathology, University of Veterinary Medicine Hannover, 30559, 
      Hannover, Germany.
FAU - Hoeltig, Doris
AU  - Hoeltig D
AD  - Clinic for Swine, Small Ruminants, Forensic Medicine and Ambulatory Services, 
      University of Veterinary Medicine Hannover, 30173, Hannover, Germany.
FAU - Postel, Alexander
AU  - Postel A
AUID- ORCID: 0000-0002-1187-1069
AD  - Institute of Virology, University of Veterinary Medicine Hannover, 30559, 
      Hannover, Germany.
FAU - Keuling, Oliver
AU  - Keuling O
AUID- ORCID: 0000-0002-9052-6860
AD  - Institute for Terrestrial and Aquatic Wildlife Research, University of Veterinary 
      Medicine Hannover, 30173, Hannover, Germany.
FAU - Ciurkiewicz, Malgorzata
AU  - Ciurkiewicz M
AUID- ORCID: 0000-0001-6277-7366
AD  - Department of Pathology, University of Veterinary Medicine Hannover, 30559, 
      Hannover, Germany.
FAU - Baumgartner, Wolfgang
AU  - Baumgartner W
AD  - Department of Pathology, University of Veterinary Medicine Hannover, 30559, 
      Hannover, Germany.
FAU - Becher, Paul
AU  - Becher P
AUID- ORCID: 0000-0001-7857-1354
AD  - Institute of Virology, University of Veterinary Medicine Hannover, 30559, 
      Hannover, Germany. paul.becher@tiho-hannover.de.
AD  - German Center for Infection Research (DZIF), Partner Site Hannover-Braunschweig, 
      30559, Hannover, Germany. paul.becher@tiho-hannover.de.
FAU - Baechlein, Christine
AU  - Baechlein C
AD  - Institute of Virology, University of Veterinary Medicine Hannover, 30559, 
      Hannover, Germany. christine.baechlein@tiho-hannover.de.
AD  - German Center for Infection Research (DZIF), Partner Site Hannover-Braunschweig, 
      30559, Hannover, Germany. christine.baechlein@tiho-hannover.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190603
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Asia
MH  - China
MH  - Europe
MH  - Flaviviridae/*genetics/pathogenicity
MH  - Flaviviridae Infections/*genetics/virology
MH  - Genome, Viral/genetics
MH  - Germany
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukocytes, Mononuclear/metabolism/virology
MH  - Phylogeny
MH  - RNA, Viral/genetics
MH  - Sus scrofa/*genetics/virology
MH  - Swine/genetics/virology
MH  - Tropism/*genetics
PMC - PMC6547670
COIS- The authors declare no competing interests.
EDAT- 2019/06/05 06:00
MHDA- 2020/10/21 06:00
PMCR- 2019/06/03
CRDT- 2019/06/05 06:00
PHST- 2018/11/29 00:00 [received]
PHST- 2019/05/21 00:00 [accepted]
PHST- 2019/06/05 06:00 [entrez]
PHST- 2019/06/05 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
PHST- 2019/06/03 00:00 [pmc-release]
AID - 10.1038/s41598-019-44642-0 [pii]
AID - 44642 [pii]
AID - 10.1038/s41598-019-44642-0 [doi]
PST - epublish
SO  - Sci Rep. 2019 Jun 3;9(1):8174. doi: 10.1038/s41598-019-44642-0.