PMID- 31161612
OWN - NLM
STAT- MEDLINE
DCOM- 20200720
LR  - 20200720
IS  - 1469-445X (Electronic)
IS  - 0958-0670 (Linking)
VI  - 104
IP  - 9
DP  - 2019 Sep
TI  - Selective denervation of the aortic and carotid baroreceptors in rats.
PG  - 1335-1342
LID - 10.1113/EP087764 [doi]
AB  - NEW FINDINGS: What is the central question of this study? The traditional 
      surgical approach for sino-aortic denervation in rats leads to simultaneous 
      carotid baroreceptor and chemoreceptor deactivation, which does not permit their 
      individual study in different situations. What is the main finding and its 
      importance? We have described a new surgical approach capable of selective 
      denervation of the arterial (aortic and carotid) baroreceptors, keeping the 
      carotid bodies (chemoreceptors) intact. It is understood that this technique 
      might be a useful tool for investigating the relative role of the baro- and 
      chemoreceptors in several physiological and pathophysiological conditions. 
      ABSTRACT: Studies have demonstrated that the traditional surgical approach for 
      sino-aortic denervation in rats leads to simultaneous carotid baroreceptor and 
      chemoreceptor deactivation. The present study reports a new surgical approach to 
      denervate the aortic and the carotid baroreceptors selectively, keeping the 
      carotid bodies (peripheral chemoreceptors) intact. Wistar rats were subjected to 
      specific aortic and carotid baroreceptor denervation (BAROS-X) or sham surgery 
      (SHAM). Baroreflex activation was achieved by i.v. administration of 
      phenylephrine, whereas peripheral chemoreflex activation was produced by i.v. 
      administration of potassium cyanide. The SHAM and BAROS-X rats displayed 
      significant hypertensive responses to phenylephrine administration. However, the 
      reflex bradycardia following the hypertensive response caused by phenylephrine 
      was remarkable in SHAM, but not significant in the BAROS-X animals, confirming 
      the efficacy of the surgical procedure to abolish the baroreflex. In addition, 
      the baroreflex activation elicited by phenylephrine increased carotid sinus nerve 
      activity only in SHAM, but not in the BAROS-X animals, providing support to the 
      notion that the baroreceptor afferents were absent. Instead, the classical 
      peripheral chemoreflex hypertensive and bradycardic responses to potassium 
      cyanide were similar in both groups, suggesting that the carotid body 
      chemoreceptors were preserved after BAROS-X. In summary, we describe a new 
      surgical approach in which only the baroreceptors are eliminated, while the 
      carotid chemoreceptors are preserved. Therefore, it is understood that this 
      procedure is potentially a useful tool for examining the relative roles of the 
      arterial baroreceptors versus the chemoreceptors in several pathophysiological 
      conditions, for instance, arterial hypertension and heart failure.
CI  - (c) 2019 The Authors. Experimental Physiology (c) 2019 The Physiological Society.
FAU - Castania, Jaci A
AU  - Castania JA
AD  - Department of Physiology, Ribeirao Preto Medical School, University of Sao Paulo, 
      Ribeirao Preto, Sao Paulo, Brazil.
FAU - Katayama, Pedro L
AU  - Katayama PL
AUID- ORCID: 0000-0001-5641-7305
AD  - Department of Physiology, Ribeirao Preto Medical School, University of Sao Paulo, 
      Ribeirao Preto, Sao Paulo, Brazil.
FAU - Brognara, Fernanda
AU  - Brognara F
AUID- ORCID: 0000-0002-5042-1319
AD  - Department of Physiology, Ribeirao Preto Medical School, University of Sao Paulo, 
      Ribeirao Preto, Sao Paulo, Brazil.
FAU - Moraes, Davi J A
AU  - Moraes DJA
AD  - Department of Physiology, Ribeirao Preto Medical School, University of Sao Paulo, 
      Ribeirao Preto, Sao Paulo, Brazil.
FAU - Sabino, Joao Paulo J
AU  - Sabino JPJ
AD  - Department of Biophysics and Physiology, Federal University of Piaui, Teresina, 
      Piaui, Brazil.
FAU - Salgado, Helio C
AU  - Salgado HC
AD  - Department of Physiology, Ribeirao Preto Medical School, University of Sao Paulo, 
      Ribeirao Preto, Sao Paulo, Brazil.
LA  - eng
GR  - #2013/20549-7/Sao Paulo Research Foundation/International
GR  - #2013/10484-5/Sao Paulo Research Foundation/International
GR  - #2015/18511-7/Sao Paulo Research Foundation/International
GR  - #2017/05163-6/Sao Paulo Research Foundation/International
GR  - #475715/2012-8/National Council for Scientific and Technological 
      Development/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190707
PL  - England
TA  - Exp Physiol
JT  - Experimental physiology
JID - 9002940
RN  - 1WS297W6MV (Phenylephrine)
SB  - IM
MH  - Animals
MH  - Aorta/drug effects/physiology/*surgery
MH  - Arteries/drug effects/*surgery
MH  - Baroreflex/drug effects/physiology
MH  - Blood Pressure/drug effects/physiology
MH  - Carotid Body/drug effects/physiology/*surgery
MH  - Chemoreceptor Cells/drug effects/physiology
MH  - Denervation/methods
MH  - Heart Rate/drug effects/physiology
MH  - Hypertension/physiopathology
MH  - Male
MH  - Phenylephrine/pharmacology
MH  - Pressoreceptors/drug effects/physiology
MH  - Rats
MH  - Rats, Wistar
OTO - NOTNLM
OT  - autonomic nervous system
OT  - baroreflex
OT  - haemodynamics
OT  - phenylephrine
EDAT- 2019/06/05 06:00
MHDA- 2020/07/21 06:00
CRDT- 2019/06/05 06:00
PHST- 2019/03/29 00:00 [received]
PHST- 2019/06/03 00:00 [accepted]
PHST- 2019/06/05 06:00 [pubmed]
PHST- 2020/07/21 06:00 [medline]
PHST- 2019/06/05 06:00 [entrez]
AID - 10.1113/EP087764 [doi]
PST - ppublish
SO  - Exp Physiol. 2019 Sep;104(9):1335-1342. doi: 10.1113/EP087764. Epub 2019 Jul 7.