PMID- 3116371
OWN - NLM
STAT- MEDLINE
DCOM- 19871104
LR  - 20190820
IS  - 0385-5600 (Print)
IS  - 0385-5600 (Linking)
VI  - 31
IP  - 5
DP  - 1987
TI  - Human macrophage-activating factors for cytotoxicity. I. Establishment of a human 
      T-cell hybridoma that produces macrophage-activating factors for cytotoxicity.
PG  - 469-79
AB  - Human T cell hybridoma, H3-E9-6, that produces macrophage activating factors for 
      cytotoxicity (MAF-C) was prepared by somatic fusion of 
      phytohemagglutinin-activated peripheral blood lymphocytes with 
      emetine/actinomycin D-treated cloned human acute lymphocytic leukemia cells (CEM 
      11). The activities of the following were assayed: (1) macrophage-activating 
      factor for cytotoxicity of monocytes (MAF-C 1 day), (2) macrophage-activating 
      factor for cytotoxicity of monocyte-derived macrophages (MAF-C 6 day), (3) 
      macrophage-activating factor for cytotoxicity of murine macrophages (MAF-Cm), (4) 
      macrophage-activating factor for glucose consumption (MAF-G), (5) 
      macrophage-activating factor for O2- formation (MAF-O). The culture supernatant 
      of H3-E9-6 showed MAF-C 1 day-MAF-C 6 day, MAF-Cm, and MAF-G activities. The 
      MAF-Cm activity was considerably enhanced by the addition of murine recombinant 
      interferon gamma (rIFN-gamma). The MAF-C 1 day activity in the H3-E9-6 sup was 
      not decreased by heat treatment (56 C, 30 min), by pH 2 treatment or by the 
      addition of monoclonal anti-human IFN-gamma antibody or polymyxin B. These data 
      suggest that MAF-C in H3-E9-6 sup is distinct from human IFN-gamma or 
      lipopolysaccharide (LPS).
FAU - Higuchi, M
AU  - Higuchi M
AD  - Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical 
      Sciences, University of Tokyo.
FAU - Sugimoto, M
AU  - Sugimoto M
FAU - Kobayashi, Y
AU  - Kobayashi Y
FAU - Osawa, T
AU  - Osawa T
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Microbiol Immunol
JT  - Microbiology and immunology
JID - 7703966
RN  - 0 (Lymphokines)
RN  - 0 (Macrophage-Activating Factors)
RN  - 0 (Recombinant Proteins)
RN  - 82115-62-6 (Interferon-gamma)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - *Cytotoxicity, Immunologic
MH  - Glucose/metabolism
MH  - HeLa Cells
MH  - Hot Temperature
MH  - Humans
MH  - Hybridomas
MH  - Hydrogen-Ion Concentration
MH  - Interferon-gamma/pharmacology
MH  - Lymphokines/*biosynthesis/pharmacology
MH  - *Macrophage Activation
MH  - Macrophage-Activating Factors
MH  - Macrophages/*immunology
MH  - Recombinant Proteins/pharmacology
MH  - Tumor Cells, Cultured/immunology
EDAT- 1987/01/01 00:00
MHDA- 1987/01/01 00:01
CRDT- 1987/01/01 00:00
PHST- 1987/01/01 00:00 [pubmed]
PHST- 1987/01/01 00:01 [medline]
PHST- 1987/01/01 00:00 [entrez]
AID - 10.1111/j.1348-0421.1987.tb03109.x [doi]
PST - ppublish
SO  - Microbiol Immunol. 1987;31(5):469-79. doi: 10.1111/j.1348-0421.1987.tb03109.x.