PMID- 3116372
OWN - NLM
STAT- MEDLINE
DCOM- 19871104
LR  - 20190820
IS  - 0385-5600 (Print)
IS  - 0385-5600 (Linking)
VI  - 31
IP  - 5
DP  - 1987
TI  - Human macrophage-activating factors for cytotoxicity. II. Synergism of two 
      factors produced by a human T-cell hybridoma that induce the cytotoxicity of 
      human monocytes.
PG  - 481-90
AB  - Two different factors (MAF-C I and MAF-C II) were obtained by anion exchange 
      chromatography of the culture supernatant of a human T-cell hybridoma, H3-E9-6, 
      which produces macrophage-activating factors for cytotoxicity (MAF-C). These 2 
      factors induced the cytotoxicity of monocytes synergistically as a priming signal 
      (MAF-C I) and a triggering signal (MAF-C II), respectively. On gel filtration on 
      a column of Superose 12, MAF-C II was eluted mainly at the void volume, whereas 
      MAF-C I was eluted in the fractions corresponding to approximate molecular 
      weights of 30-300 K. On the other hand, gel filtration in the presence of sodium 
      deoxycholate revealed that MAF-C II has an approximate molecular weight of 
      40,000, but MAF-C I was unstable under these conditions. When the activity for 
      mouse macrophages (MAF-Cm activity) was tested, the MAF-C II fraction showed high 
      MAF-Cm activity in the presence of murine recombinant interferon gamma 
      (rIFN-gamma), but the MAF-C I fraction did not show MAF-Cm activity even in the 
      presence of lipopolysaccharide (LPS). These results suggest that MAF-C I (priming 
      lymphokine) has species specificity but MAF-C II (triggering lymphokine) does 
      not.
FAU - Higuchi, M
AU  - Higuchi M
AD  - Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical 
      Sciences, University of Tokyo.
FAU - Sugimoto, M
AU  - Sugimoto M
FAU - Kobayashi, Y
AU  - Kobayashi Y
FAU - Osawa, T
AU  - Osawa T
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Microbiol Immunol
JT  - Microbiology and immunology
JID - 7703966
RN  - 0 (Lymphokines)
RN  - 0 (Macrophage-Activating Factors)
RN  - 0 (Recombinant Proteins)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Chromatography, Gel
MH  - Chromatography, Ion Exchange
MH  - *Cytotoxicity, Immunologic
MH  - Drug Synergism
MH  - Humans
MH  - Hybridomas
MH  - Interferon-gamma/pharmacology
MH  - Lymphokines/analysis/*pharmacology
MH  - *Macrophage Activation
MH  - Macrophage-Activating Factors
MH  - Macrophages/*immunology
MH  - Monocytes/*immunology
MH  - Recombinant Proteins/pharmacology
MH  - Species Specificity
MH  - Tumor Cells, Cultured/immunology
EDAT- 1987/01/01 00:00
MHDA- 1987/01/01 00:01
CRDT- 1987/01/01 00:00
PHST- 1987/01/01 00:00 [pubmed]
PHST- 1987/01/01 00:01 [medline]
PHST- 1987/01/01 00:00 [entrez]
AID - 10.1111/j.1348-0421.1987.tb03110.x [doi]
PST - ppublish
SO  - Microbiol Immunol. 1987;31(5):481-90. doi: 10.1111/j.1348-0421.1987.tb03110.x.