PMID- 31167690
OWN - NLM
STAT- MEDLINE
DCOM- 20190905
LR  - 20190906
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 35
IP  - 4
DP  - 2019 Apr
TI  - [High glucose induces transdifferentiation of HK-2 human renal tubular epithelial 
      cells by activating reactive oxygen species-mediated NF-kappa B signaling 
      pathway].
PG  - 313-319
AB  - Objective To explore the mechanism of transdifferentiation of human renal tubular 
      epithelial cells induced by high glucose based on reactive oxygen species 
      (ROS)/NF-kappaB signaling pathway. Methods HK-2 normal human proximal tubular 
      epithelial cells were randomly divided into blank control group, osmotic pressure 
      control group, high glucose group and pyrrolidine dithiocarbamate (PDTC) group. 
      Cell morphology was observed by phase contrast microscope. Cell viability was 
      measured by MTT assay. Flow cytometry was used to detect intracellular ROS 
      content. Malondialdehyde (MDA) content and superoxide dismutase activity were 
      tested by ELISA. Western blot analysis was used to examine the protein levels of 
      NF-kappaBp65, phosphorylated IkappaBalpha (p-IkappaBalpha) and IKKalpha, monocyte chemoattractant 
      protein-1 (MCP-1) and intercellular adhesion molecule-1 (ICAM-1). The expressions 
      of NF-kappaBp65, epithelial cadherin (E-cadherin) and alpha smooth muscle actin 
      (alpha-SMA) were assessed by immunocytochemistry. Results In high glucose group, the 
      cells became spindle-shaped or irregular, with radial edges, enlarged 
      intercellular space, decreased refractive index and irregular arrangement. At the 
      same time, the cell activity decreased with the prolongation of treatment time, 
      and the cell activity in PDTC group was higher than that in high glucose group. 
      Compared with the blank control group, the content of ROS and MDA increased and 
      the activity of SOD decreased in the high glucose group, while the content of ROS 
      and MDA decreased and the activity of SOD increased in the PDTC group compared 
      with the high glucose group. Compared with the blank group, the protein levels of 
      NF-kappaBp65, p-IkappaBalpha, IKKalpha, MCP-1 and ICAM-1 increased in the high glucose group, 
      while the protein levels above decreased in the PDTC group compared with the high 
      glucose group. Compared with the blank group, the proportion of positive cells of 
      NF-kappaBp65 and alpha-SMA increased and the proportion of positive cells of E-cadherin 
      decreased in the high glucose group. Compared with the high glucose group, the 
      proportion of positive cells of NF-kappaBp65 and alpha-SMA decreased and the proportion 
      of positive cells of E-cadherin increased in the PDTC group. Conclusion High 
      glucose can induce epithelial-mesenchymal transition in HK-2 cells. Activation of 
      NF-kappaB signaling pathway mediated by ROS participates in the above process, which 
      can be blocked by PDTC.
FAU - Zhang, Lei
AU  - Zhang L
AD  - School of Graduate, Anhui University of Chinese Medicine, Hefei 230038, China.
FAU - Jin, Hua
AU  - Jin H
AD  - Department of Nephrology, First Affiliated Hospital of Anhui University of 
      Chinese Medicine, Hefei 230031, China.
FAU - Wang, Yiping
AU  - Wang Y
AD  - Department of Nephrology, First Affiliated Hospital of Anhui University of 
      Chinese Medicine, Hefei 230031, China. *Corresponding author, E-mail: 
      wypwyp54@aliyun.com.
FAU - Wang, Dong
AU  - Wang D
AD  - Department of Nephrology, First Affiliated Hospital of Anhui University of 
      Chinese Medicine, Hefei 230031, China.
FAU - Li, Zhuoya
AU  - Li Z
AD  - Department of Nephrology, Fanchang County Hospital of Traditional Chinese 
      Medicine, Wuhu 241200, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Culture Media)
RN  - 0 (NF-kappa B)
RN  - 0 (Pyrrolidines)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Thiocarbamates)
RN  - 25769-03-3 (pyrrolidine dithiocarbamic acid)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Cell Line
MH  - *Cell Transdifferentiation
MH  - Culture Media
MH  - Epithelial Cells/*cytology
MH  - Glucose
MH  - Humans
MH  - Kidney Tubules/cytology
MH  - NF-kappa B/*metabolism
MH  - Pyrrolidines
MH  - Reactive Oxygen Species/*metabolism
MH  - *Signal Transduction
MH  - Thiocarbamates
EDAT- 2019/06/07 06:00
MHDA- 2019/09/07 06:00
CRDT- 2019/06/07 06:00
PHST- 2019/06/07 06:00 [entrez]
PHST- 2019/06/07 06:00 [pubmed]
PHST- 2019/09/07 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2019 Apr;35(4):313-319.