PMID- 31168681 OWN - NLM STAT- MEDLINE DCOM- 20200518 LR - 20200824 IS - 1869-1889 (Electronic) IS - 1674-7305 (Linking) VI - 62 IP - 8 DP - 2019 Aug TI - Regulation of mitochondrial NAD pool via NAD(+) transporter 2 is essential for matrix NADH homeostasis and ROS production in Arabidopsis. PG - 991-1002 LID - 10.1007/s11427-019-9563-y [doi] AB - Reactive oxygen species (ROS) play a crucial role in numerous biological processes in plants, including development, responses to environmental stimuli, and programmed cell death (PCD). Deficiency in MOSAIC DEATH 1 (MOD1), a plastid-localized enoyl-ACP reductase essential for de novo fatty acid biosynthesis in Arabidopsis thaliana, leads to the increased malate export from chloroplasts to mitochondria, and the subsequent accumulation of mitochondria-generated ROS and PCD. In this study, we report the identification and characterization of a mod1 suppressor, som592. SOM592 encodes mitochondrion-localized NAD+ transporter 2 (NDT2). We show that the mitochondrial NAD pool is elevated in the mod1 mutant. The som592 mutation fully suppressed mitochondrial NADH hyper-accumulation, ROS production, and PCD in the mod1 mutant, indicating a causal relationship between mitochondrial NAD accumulation and ROS/PCD phenotypes. We also show that in wild-type plants, the mitochondrial NAD(+) uptake is involved in the regulation of ROS production in response to continuous photoperiod. Elevation of the alternative respiration pathway can suppress ROS accumulation and PCD in mod1, but leads to growth restriction. These findings uncover a regulatory mechanism for mitochondrial ROS production via NADH homeostasis in Arabidopsis thaliana that is likely important for growth regulation in response to altered photoperiod. FAU - Luo, Lilan AU - Luo L AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China. FAU - He, Yajun AU - He Y AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China. AD - University of Chinese Academy of Sciences, Beijing, 100049, China. FAU - Zhao, Yannan AU - Zhao Y AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China. FAU - Xu, Qian AU - Xu Q AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China. AD - University of Chinese Academy of Sciences, Beijing, 100049, China. FAU - Wu, Jian AU - Wu J AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China. FAU - Ma, Haiyan AU - Ma H AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China. FAU - Guo, Hongyan AU - Guo H AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China. FAU - Bai, Lin AU - Bai L AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China. AD - University of Chinese Academy of Sciences, Beijing, 100049, China. FAU - Zuo, Jianru AU - Zuo J AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China. AD - University of Chinese Academy of Sciences, Beijing, 100049, China. FAU - Zhou, Jian-Min AU - Zhou JM AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China. AD - University of Chinese Academy of Sciences, Beijing, 100049, China. FAU - Yu, Hong AU - Yu H AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China. hyu@genetics.ac.cn. FAU - Li, Jiayang AU - Li J AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China. jyli@genetics.ac.cn. AD - University of Chinese Academy of Sciences, Beijing, 100049, China. jyli@genetics.ac.cn. LA - eng PT - Journal Article DEP - 20190604 PL - China TA - Sci China Life Sci JT - Science China. Life sciences JID - 101529880 RN - 0 (Arabidopsis Proteins) RN - 0 (At1g25380 protein, Arabidopsis) RN - 0 (Malates) RN - 0 (Mitochondrial Proteins) RN - 0 (Nucleotide Transport Proteins) RN - 0 (Reactive Oxygen Species) RN - 0U46U6E8UK (NAD) RN - 817L1N4CKP (malic acid) RN - EC 2.3.1.85 (Fatty Acid Synthases) SB - IM MH - Arabidopsis/*chemistry MH - Arabidopsis Proteins/*metabolism MH - Biological Transport MH - Chloroplasts/metabolism MH - Cloning, Molecular MH - Fatty Acid Synthases/metabolism MH - Gene Expression Regulation, Plant MH - Homeostasis MH - Malates/metabolism MH - Mitochondria/*metabolism MH - Mitochondrial Proteins/*metabolism MH - Mutation MH - NAD/*metabolism MH - Nucleotide Transport Proteins/*metabolism MH - Reactive Oxygen Species/*metabolism OTO - NOTNLM OT - NAD+ transporter 2 OT - mitochondrial NAD pool OT - mtROS EDAT- 2019/06/07 06:00 MHDA- 2020/05/19 06:00 CRDT- 2019/06/07 06:00 PHST- 2019/04/05 00:00 [received] PHST- 2019/05/10 00:00 [accepted] PHST- 2019/06/07 06:00 [pubmed] PHST- 2020/05/19 06:00 [medline] PHST- 2019/06/07 06:00 [entrez] AID - 10.1007/s11427-019-9563-y [pii] AID - 10.1007/s11427-019-9563-y [doi] PST - ppublish SO - Sci China Life Sci. 2019 Aug;62(8):991-1002. doi: 10.1007/s11427-019-9563-y. Epub 2019 Jun 4.