PMID- 31168933
OWN - NLM
STAT- MEDLINE
DCOM- 20200622
LR  - 20210109
IS  - 2045-8827 (Electronic)
IS  - 2045-8827 (Linking)
VI  - 8
IP  - 10
DP  - 2019 Oct
TI  - Diversity, enumeration, and isolation of Arcobacter spp. in the giant abalone, 
      Haliotis gigantea.
PG  - e890
LID - 10.1002/mbo3.890 [doi]
LID - e890
AB  - Arcobacter have been frequently detected in and isolated from bivalves, but there 
      is very little information on the genus Arcobacter in the abalone, an important 
      fishery resource. This study aimed to investigate the genetic diversity and 
      abundance of bacteria from the genus Arcobacter in the Japanese giant abalone, 
      Haliotis gigantea, using molecular methods such as Arcobacter-specific clone 
      libraries and fluorescence in situ hybridization (FISH). Furthermore, we 
      attempted to isolate the Arcobacter species detected. Twelve genotypes of clones 
      were obtained from Arcobacter-specific clone libraries. These sequences are not 
      classified with any other known Arcobacter species including pathogenic 
      Arcobacter spp., A. butzleri, A. skirrowii, and A. cryaerophilus, commonly 
      isolated or detected from bivalves. From the FISH analysis, we observed that 
      ARC94F-positive cells, presumed to be Arcobacter, accounted for 6.96 +/- 0.72% of 
      all EUB338-positive cells. In the culture method, three genotypes of Arcobacter 
      were isolated from abalones. One genotype had a similarity of 99.2%-100.0% to the 
      16S rRNA gene of Arcobacter marinus, while the others showed only 93.3%-94.3% 
      similarity to other Arcobacter species. These data indicate that abalones carry 
      Arcobacter as a common bacterial genus which includes uncultured species.
CI  - (c) 2019 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
FAU - Mizutani, Yukino
AU  - Mizutani Y
AD  - Graduate School of Bioresources, Laboratory of Marine Microbiology, Mie 
      University, Tsu, Japan.
FAU - Iehata, Shunpei
AU  - Iehata S
AD  - School of Fisheries and Aquaculture Science, Universiti Malaysia Terengganu, 
      Kuala Terengganu, Terengganu, Malaysia.
FAU - Mori, Tetsushi
AU  - Mori T
AD  - Department of Biotechnology and Life Science, Tokyo University of Agriculture and 
      Technology, Koganei, Japan.
FAU - Oh, Ryota
AU  - Oh R
AD  - Graduate School of Bioresources, Laboratory of Marine Microbiology, Mie 
      University, Tsu, Japan.
FAU - Fukuzaki, Satoshi
AU  - Fukuzaki S
AD  - Graduate School of Bioresources, Laboratory of Marine Microbiology, Mie 
      University, Tsu, Japan.
FAU - Tanaka, Reiji
AU  - Tanaka R
AUID- ORCID: 0000-0002-7459-0635
AD  - Graduate School of Bioresources, Laboratory of Marine Microbiology, Mie 
      University, Tsu, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190605
PL  - England
TA  - Microbiologyopen
JT  - MicrobiologyOpen
JID - 101588314
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Arcobacter/*classification/genetics/*isolation & purification
MH  - *Biodiversity
MH  - Cluster Analysis
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Gastropoda/*microbiology
MH  - Genotype
MH  - In Situ Hybridization, Fluorescence
MH  - Metagenomics
MH  - *Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
PMC - PMC6813453
OTO - NOTNLM
OT  - Arcobacter
OT  - abalone
OT  - clone libraries
OT  - cultivation
OT  - fluorescent in situ hybridization
COIS- All authors declare no conflict of interest.
EDAT- 2019/06/07 06:00
MHDA- 2020/06/23 06:00
PMCR- 2019/06/05
CRDT- 2019/06/07 06:00
PHST- 2019/02/14 00:00 [received]
PHST- 2019/05/16 00:00 [revised]
PHST- 2019/05/20 00:00 [accepted]
PHST- 2019/06/07 06:00 [pubmed]
PHST- 2020/06/23 06:00 [medline]
PHST- 2019/06/07 06:00 [entrez]
PHST- 2019/06/05 00:00 [pmc-release]
AID - MBO3890 [pii]
AID - 10.1002/mbo3.890 [doi]
PST - ppublish
SO  - Microbiologyopen. 2019 Oct;8(10):e890. doi: 10.1002/mbo3.890. Epub 2019 Jun 5.