PMID- 31168988
OWN - NLM
STAT- MEDLINE
DCOM- 20190826
LR  - 20231113
IS  - 2618-0456 (Electronic)
IS  - 1000-1182 (Print)
IS  - 1000-1182 (Linking)
VI  - 37
IP  - 2
DP  - 2019 Apr 1
TI  - [MicroRNA-29a-3p regulates osteoblast differentiation and peri-implant 
      osseointegration in a rat model of hyperlipidemia by modulating Frizzled 4 
      expression].
PG  - 200-207
LID - 10.7518/hxkq.2019.02.013 [doi]
AB  - OBJECTIVE: This work aimed to study and identify the influence and target gene of 
      microRNA-29a-3p (miR-29a-3p) in the osteogenic differentiation of bone marrow 
      mesenchymal stem cells (BMSCs) in a high-fat environment in vitro and in vivo. 
      METHODS: 1) In vitro: BMSCs were randomly allocated into two groups and were then 
      induced to undergo osteogenic differentiation in a normal or high-fat 
      environment. Next, a miR-29a-3p mimic/inhibitor was transfected into the two 
      groups of cells. The mRNA expression levels of alkaline phosphatase (ALP), Runt 
      related gene 2 (Runx2), and miR-29a-3p and the protein expression levels of ALP 
      and Runx2 were detected before and after transfection through reverse 
      transcription-quantitative real-time polymerase chain reaction (RT-qPCR) and 
      Western blot analyses. Moreover, Frizzled (Fzd) 4 was predicted as the target 
      gene of miR-29a-3p by using an online database (Target Scan, MiRNA.org). The 
      interactive relationship between miR-29a-3p and Fzd4 was confirmed through 
      dual-luciferase assays. 2) In vivo: Rats were randomly divided into two groups 
      and fed with a standard or high-fat diet. Titanium implants were grown in rats. 
      Then, the expression levels of miR-29a-3p, ALP, and Runx2 were detected in bone 
      tissues surrounding implants. Moreover, hard tissue sections were subjected to 
      methylene blue-acid magenta staining and observed under microscopy to study bone 
      formation around implants. In addition, miR-29a-3p-overexpressing lentiviral 
      vectors were transfected into rats, and the expression levels of ALP, Runx2, and 
      miR-29a-3p in bone tissues surrounding implants were detected at 3 and 10 days 
      after transfection. RESULTS: The expression levels of ALP, Runx2, and miR-29a-3p 
      and the osteogenic differentiation of BMSCs were suppressed in high-fat groups in 
      vitro and in vivo. CONCLUSIONS: MiR-29a-3p plays a positive role in the 
      regulation of BMSCs in a high-fat environment. It can increase ALP and Runx2 
      expression levels in bone tissues surrounding implants in hyperlipidemia models. 
      This result implies that miR-29a-3p can promote implant osseointergration in a 
      rat model of hyperlipidemia.
FAU - Liu, Fei
AU  - Liu F
AD  - Dept. of Prosthodontics, School of Stomatology, Shandong University, Shandong 
      Provincial Key Laboratory of Oral Tissue Regeneration, Jinan 250012, China.
FAU - Wang, Zhi-Feng
AU  - Wang ZF
AD  - Dept. of Pediatric Dentistry, School of Stomatology, Shandong University, 
      Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan 250012, 
      China.
FAU - Liu, Fang-Fang
AU  - Liu FF
AD  - Dept. of Dental Implantology, Stomatological Hospital of Nanyang, Nanyang 473000, 
      China.
FAU - Xu, Jin-Zhao
AU  - Xu JZ
AD  - Dept. of Pediatric Dentistry, School of Stomatology, Shandong University, 
      Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan 250012, 
      China.
FAU - Liu, Qibo
AU  - Liu Q
AD  - Dept. of Prosthodontics, School of Stomatology, Shandong University, Shandong 
      Provincial Key Laboratory of Oral Tissue Regeneration, Jinan 250012, China.
FAU - Lan, Jing
AU  - Lan J
AD  - Dept. of Prosthodontics, School of Stomatology, Shandong University, Shandong 
      Provincial Key Laboratory of Oral Tissue Regeneration, Jinan 250012, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Hua Xi Kou Qiang Yi Xue Za Zhi
JT  - Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal 
      of stomatology
JID - 9422648
RN  - 0 (Dental Implants)
RN  - 0 (MicroRNAs)
MH  - Animals
MH  - *Cell Differentiation
MH  - *Dental Implants
MH  - *Hyperlipidemias
MH  - *MicroRNAs
MH  - Osseointegration
MH  - Osteoblasts
MH  - *Osteogenesis
MH  - Random Allocation
MH  - Rats
PMC - PMC7030145
OTO - NOTNLM
OT  - Frizzled 4
OT  - dual luciferase reporter gene
OT  - hyperlipidemia
OT  - microRNA-29a-3p
OT  - osteointegration
EDAT- 2019/06/07 06:00
MHDA- 2019/08/27 06:00
PMCR- 2019/04/01
CRDT- 2019/06/07 06:00
PHST- 2019/06/07 06:00 [entrez]
PHST- 2019/06/07 06:00 [pubmed]
PHST- 2019/08/27 06:00 [medline]
PHST- 2019/04/01 00:00 [pmc-release]
AID - wcjs-37-02-200 [pii]
AID - 10.7518/hxkq.2019.02.013 [doi]
PST - ppublish
SO  - Hua Xi Kou Qiang Yi Xue Za Zhi. 2019 Apr 1;37(2):200-207. doi: 
      10.7518/hxkq.2019.02.013.