PMID- 31169312
OWN - NLM
STAT- MEDLINE
DCOM- 20200612
LR  - 20200612
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 234
IP  - 12
DP  - 2019 Dec
TI  - Upregulation of microRNA-9-5p inhibits apoptosis of chondrocytes through 
      downregulating Tnc in mice with osteoarthritis following tibial plateau fracture.
PG  - 23326-23336
LID - 10.1002/jcp.28900 [doi]
AB  - Osteoarthritis (OA) is a common degenerative joint disease which is typically 
      progressed with age, affecting smaller joints of hands, lower limbs, and the 
      vertebral column. It has been reported that microRNAs could regulate the 
      biological processes of OA. Therefore, the purpose of this study was to elucidate 
      miR-9-5p's role in regulating cartilage remodeling of OA mice following tibial 
      plateau fracture (TPF) through regulation of tenascin C (Tnc). Initially, we 
      determined the expression of miR-9-5p and Tnc in mice with OA and then testified 
      their relationship. The results displayed a high expression of Tnc, but a poor 
      expression of miR-9-5p with high methylation in OA. Tnc was confirmed to be a 
      target gene of miR-9-5p. Moreover, based on gain- and loss-function experiments, 
      an increase of miR-9-5p and loss of Tnc had the potential to inhibit cell 
      apoptosis, while facilitating cell proliferation, migration, invasion, and 
      cartilage remodeling of mice with OA following TPF. This was further demonstrated 
      by a higher expression of type II collagen, lower type X collagen, and protogenin 
      expression. Subsequently, downregulation of miR-9-5p aggravated the pathological 
      changes of mice, illustrated by an increase in the Mankin score. In conclusion, 
      the present study proved that overexpression of miR-9-5p suppressed chondrocytes 
      apoptosis and promoted cartilage remodeling through downregulating of Tnc in mice 
      with OA.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Chen, Hongwei
AU  - Chen H
AD  - Department of Orthopedics, Yiwu Central Hospital, Affiliated Hospital of Wenzhou 
      Medical University, Yiwu, People's Republic of China.
FAU - Yang, Jun
AU  - Yang J
AD  - Department of Orthopedics, The Affiliated Hospital of Xinyang Vocational and 
      Technical College, Xinyang, People's Republic of China.
FAU - Tan, Zhihong
AU  - Tan Z
AUID- ORCID: 0000-0003-4311-8677
AD  - Department of Orthopaedics, Huizhou Hospital of Traditional Chinese Medicine, 
      Huizhou, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20190606
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (MIRN9 microRNA, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (Tenascin)
RN  - 0 (Tnc protein, mouse)
SB  - IM
MH  - Animals
MH  - Apoptosis/physiology
MH  - Chondrocytes/*metabolism/pathology
MH  - Down-Regulation
MH  - Female
MH  - Gene Expression Regulation/*genetics
MH  - Knee Joint/metabolism/pathology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/*metabolism
MH  - Osteoarthritis/etiology/*metabolism/pathology
MH  - Tenascin/*metabolism
MH  - Tibial Fractures/complications/metabolism/pathology
MH  - Up-Regulation
OTO - NOTNLM
OT  - Tnc
OT  - cartilage remodeling
OT  - microRNA-9-5p
OT  - osteoarthritis
OT  - tibial plateau fracture
EDAT- 2019/06/07 06:00
MHDA- 2020/06/13 06:00
CRDT- 2019/06/07 06:00
PHST- 2019/01/14 00:00 [received]
PHST- 2019/05/12 00:00 [revised]
PHST- 2019/05/14 00:00 [accepted]
PHST- 2019/06/07 06:00 [pubmed]
PHST- 2020/06/13 06:00 [medline]
PHST- 2019/06/07 06:00 [entrez]
AID - 10.1002/jcp.28900 [doi]
PST - ppublish
SO  - J Cell Physiol. 2019 Dec;234(12):23326-23336. doi: 10.1002/jcp.28900. Epub 2019 
      Jun 6.