PMID- 31169331 OWN - NLM STAT- MEDLINE DCOM- 20190813 LR - 20210109 IS - 1349-7006 (Electronic) IS - 1347-9032 (Print) IS - 1347-9032 (Linking) VI - 110 IP - 8 DP - 2019 Aug TI - Vascular endothelial growth factor (VEGF) impairs the motility and immune function of human mature dendritic cells through the VEGF receptor 2-RhoA-cofilin1 pathway. PG - 2357-2367 LID - 10.1111/cas.14091 [doi] AB - Dendritic cells (DCs) are potent and specialized antigen presenting cells, which play a crucial role in initiating and amplifying both the innate and adaptive immune responses against cancer. Tumor cells can escape from immune attack by secreting suppressive cytokines that solely or cooperatively impair the immune function of DCs. However, the underlying mechanisms are not fully defined. Vascular endothelial growth factor (VEGF) has been identified as a major cytokine in the tumor microenvironment. To elucidate the effects of VEGF on the motility and immune function of mature DCs (mDCs), the cells were treated with 50 ng/mL VEGF and investigated by proteomics and molecular biological technologies. The results showed that VEGF can impair the migration capacity and immune function of mDCs through the RhoA-cofilin1 pathway mediated by the VEGF receptor 2, suggesting impaired motility of mDCs by VEGF is one of the aspects of immune escape mechanisms of tumors. It is clinically important to understand the biological behavior of DCs and the immune escape mechanisms of tumor as well as how to improve the efficiency of antitumor therapy based on DCs. CI - (c) 2019 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. FAU - Long, Jinhua AU - Long J AD - Department of Immunology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang, China. AD - Immune Cells and Antibody Engineering Research Center of Guizhou Province, Key Laboratory of Biology and Medical Engineering, School of Biology and Engineering, Guizhou Medical University, Guiyang, China. AD - Department of Head and Neck, Affiliated Tumor Hospital, Guizhou Medical University, Guiyang, China. FAU - Hu, Zuquan AU - Hu Z AD - Department of Immunology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang, China. AD - Immune Cells and Antibody Engineering Research Center of Guizhou Province, Key Laboratory of Biology and Medical Engineering, School of Biology and Engineering, Guizhou Medical University, Guiyang, China. AD - Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang, China. FAU - Xue, Hui AU - Xue H AD - Immune Cells and Antibody Engineering Research Center of Guizhou Province, Key Laboratory of Biology and Medical Engineering, School of Biology and Engineering, Guizhou Medical University, Guiyang, China. FAU - Wang, Yun AU - Wang Y AD - Department of Immunology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang, China. AD - Immune Cells and Antibody Engineering Research Center of Guizhou Province, Key Laboratory of Biology and Medical Engineering, School of Biology and Engineering, Guizhou Medical University, Guiyang, China. FAU - Chen, Jin AU - Chen J AD - Department of Immunology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang, China. AD - Immune Cells and Antibody Engineering Research Center of Guizhou Province, Key Laboratory of Biology and Medical Engineering, School of Biology and Engineering, Guizhou Medical University, Guiyang, China. FAU - Tang, Fuzhou AU - Tang F AD - Immune Cells and Antibody Engineering Research Center of Guizhou Province, Key Laboratory of Biology and Medical Engineering, School of Biology and Engineering, Guizhou Medical University, Guiyang, China. FAU - Zhou, Jing AU - Zhou J AD - Immune Cells and Antibody Engineering Research Center of Guizhou Province, Key Laboratory of Biology and Medical Engineering, School of Biology and Engineering, Guizhou Medical University, Guiyang, China. FAU - Liu, Lina AU - Liu L AD - Immune Cells and Antibody Engineering Research Center of Guizhou Province, Key Laboratory of Biology and Medical Engineering, School of Biology and Engineering, Guizhou Medical University, Guiyang, China. FAU - Qiu, Wei AU - Qiu W AD - Immune Cells and Antibody Engineering Research Center of Guizhou Province, Key Laboratory of Biology and Medical Engineering, School of Biology and Engineering, Guizhou Medical University, Guiyang, China. FAU - Zhang, Shichao AU - Zhang S AUID- ORCID: 0000-0002-8760-3482 AD - Immune Cells and Antibody Engineering Research Center of Guizhou Province, Key Laboratory of Biology and Medical Engineering, School of Biology and Engineering, Guizhou Medical University, Guiyang, China. FAU - Ouyang, Yan AU - Ouyang Y AD - Immune Cells and Antibody Engineering Research Center of Guizhou Province, Key Laboratory of Biology and Medical Engineering, School of Biology and Engineering, Guizhou Medical University, Guiyang, China. FAU - Ye, Yuannong AU - Ye Y AD - Immune Cells and Antibody Engineering Research Center of Guizhou Province, Key Laboratory of Biology and Medical Engineering, School of Biology and Engineering, Guizhou Medical University, Guiyang, China. FAU - Xu, Guoqiang AU - Xu G AD - Department of Immunology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang, China. FAU - Li, Long AU - Li L AD - Department of Nephrology, The Third Affiliated Hospital of Guizhou Medical University, Duyun, China. FAU - Zeng, Zhu AU - Zeng Z AUID- ORCID: 0000-0003-1989-5142 AD - Department of Immunology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang, China. AD - Immune Cells and Antibody Engineering Research Center of Guizhou Province, Key Laboratory of Biology and Medical Engineering, School of Biology and Engineering, Guizhou Medical University, Guiyang, China. AD - Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang, China. LA - eng GR - 31771014/National Natural Science Foundation of China/ GR - 31660258/National Natural Science Foundation of China/ GR - 31860262/National Natural Science Foundation of China/ GR - 11762006/National Natural Science Foundation of China/ GR - 2015M582747XB/China Postdoctoral Science Foundation/ GR - 210196/Key Project of the Ministry of Education of China/ GR - 2018-1412/Science and Technology Foundation of Guizhou Province/ GR - 2019-1266/Science and Technology Foundation of Guizhou Province/ GR - 2019-2787/Science and Technology Foundation of Guizhou Province/ GR - 2016-5676/Science and Technology Foundation of Guizhou Province/ GR - 2017-5718/Science and Technology Foundation of Guizhou Province/ GR - 2015-4021/Science and Technology Innovative Talent Team of Guizhou Province/ GR - 2015-04/2011 Collaborative Innovation Program of Guizhou Province/ GR - KY-2016-031/Cell and Gene Engineering Innovative Research Groups of Guizhou Province/ PT - Journal Article DEP - 20190628 PL - England TA - Cancer Sci JT - Cancer science JID - 101168776 RN - 0 (Actin Depolymerizing Factors) RN - 0 (Cytokines) RN - 0 (VEGFA protein, human) RN - 0 (Vascular Endothelial Growth Factor A) RN - 124671-05-2 (RHOA protein, human) RN - EC 2.7.10.1 (KDR protein, human) RN - EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-2) RN - EC 3.6.5.2 (rhoA GTP-Binding Protein) SB - IM MH - Actin Depolymerizing Factors/immunology/*metabolism MH - Cell Movement/*immunology MH - Cells, Cultured MH - Cytokines/immunology MH - Dendritic Cells/immunology/*metabolism MH - Human Umbilical Vein Endothelial Cells MH - Humans MH - Signal Transduction/*immunology MH - Vascular Endothelial Growth Factor A/*immunology MH - Vascular Endothelial Growth Factor Receptor-2/immunology/*metabolism MH - rhoA GTP-Binding Protein/immunology/*metabolism PMC - PMC6676124 OTO - NOTNLM OT - immune function OT - mature dendritic cell OT - motility OT - signaling pathway OT - vascular endothelial growth factor EDAT- 2019/06/07 06:00 MHDA- 2019/08/14 06:00 PMCR- 2019/08/01 CRDT- 2019/06/07 06:00 PHST- 2019/02/25 00:00 [received] PHST- 2019/06/02 00:00 [revised] PHST- 2019/06/04 00:00 [accepted] PHST- 2019/06/07 06:00 [pubmed] PHST- 2019/08/14 06:00 [medline] PHST- 2019/06/07 06:00 [entrez] PHST- 2019/08/01 00:00 [pmc-release] AID - CAS14091 [pii] AID - 10.1111/cas.14091 [doi] PST - ppublish SO - Cancer Sci. 2019 Aug;110(8):2357-2367. doi: 10.1111/cas.14091. Epub 2019 Jun 28.